Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F90 I4 R1 13 51.2 939505 89.5% 840856 286.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,442,896 A→T F401Y (TTC→TAC)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,442,8960AT97.6% 136.5 / NA 43F401Y (TTC→TAC) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (20/21);  minor base G (0/1);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTG  >  NC_000913/3442599‑3443187
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   
aGAAAAATATGCGAAAAAATCCACCTTGGCGCTGTTTATTCTTCGGCTCGGAGATGCAAATCACACGGATGACACCATTACGCTTAAAGATTTTTCAGCTACTGCATACTTTCTTGACGGAAACACGAACGTTCACTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCATTTCACTTTCGGCTTCTTCAATGCAGACGCTTACTAACTGGACATCATCAGAGTTTGCACTTGATCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGt                                                                                                                                                                                                                                                                                                  <  1:368215/301‑1 (MQ=255)
 aaaaaaCTTGCGAAAAAACCGGCCCTGGCGCTTTTTATTCTTCGGTCTGGACCTGCAAATCCCACGGATGACCCCATCACGCTTAACTATTGTGAAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGCCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCgtagta                                                                                                                                                                                                                                                                                                 <  1:358189/300‑1 (MQ=255)
      acaggtggaaaaaTCAGGCTTGG‑TCTTTGTCTGCTTCGTGTCGGCACTGCAAAACTAACAGATGAAACCATCAAGCTGAACGATGTTGCAGTTACAGCATAATTTCTTTACGGAAGCACGACCTTTCATTTTTAATCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACgg                                                                                                                                                                                                                                                                                           <  1:158176/293‑1 (MQ=255)
         tGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGTCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTCCATTGCATACTCATACTGACTGGACATCATTAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACTACCACAAAGATAAGCCGGAAGGCCCCACCCAAGAAAAACGGt                                                                                                                                                                                                                                                                                          >  1:247520/1‑300 (MQ=255)
                                      tGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGTCATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGCCAACAACGATAAGCAGTGAGGTCCAACCGTAGTAGAACTGTACTTTCATTGCATCACGCATGAACTCCggg                                                                                                                                                                                                                                                            >  2:75048/1‑301 (MQ=255)
                                           cggtccggcACTGCAACTCACACGGATGACACCATAACGCTTGACGATTTTGCAGGTACGGCAGAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCACCGTAACTGCTCGGGCGACCAATTATCGGCCGTAGCCTTTCTGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATTAGAGTTTGTACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAg                                                                                                                                                                                                                                                       <  1:38491/296‑1 (MQ=255)
                                            ggctcggcACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCACACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAgg                                                                                                                                                                                                                                                      >  2:43260/1‑301 (MQ=255)
                                                       acAACTCACACGGATGACACCATCACGCGTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAATCACGAACTCTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGt                                                                                                                                                                                                                                           <  2:147464/300‑1 (MQ=255)
                                                        cAAATCACACGGATGACACCATCACGCTTAACCATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTa                                                                                                                                                                                                                                          <  1:43248/301‑1 (MQ=255)
                                                          aaTCCCACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAATTCCGGGATCAGGCAGATAAAGGTAat                                                                                                                                                                                                                                        <  2:311891/301‑1 (MQ=255)
                                                                        cacCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCCCGCATGAACCCCGGGATCAGGCAGATAAAGGTAATAAACAGCGCacca                                                                                                                                                                                                                           >  1:104367/1‑300 (MQ=255)
                                                                          ccATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGAGCaccaac                                                                                                                                                                                                                         >  2:135550/1‑300 (MQ=255)
                                                                          ccATCACCATTAATGAGTTTCCGTTTACTGCCTTATTTCTTGACGTAAGCACGAACTGACATTTTTCCCCTCCGTTAATTCTCGCGCGACAAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTTTTCAATGCAGACTCATACGGACTGGACAACATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCaccaacc                                                                                                                                                                                                                        <  1:99437/301‑1 (MQ=255)
                                                                                cGCTTAACGATTTTGCAGTTACGGCATAATTTCTTTACGGAAGCACGAACTTTCATTTTTACTTTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTc                                                                                                                                                                                                                  <  2:281614/301‑1 (MQ=255)
                                                                                cGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAAc                                                                                                                                                                                                                                                                  >  2:76395/1‑253 (MQ=255)
                                                                                  cTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTCAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGAAAAAGGTAATATACAGCGCACCAACCAGGGTc                                                                                                                                                                                                                  >  1:3178/1‑299 (MQ=255)
                                                                                       cGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCggg                                                                                                                                                                                                           <  2:3178/301‑1 (MQ=255)
                                                                                                                       gcaacacttaCTTTCATTTTTACTCGCCGTGACTTCTCGGTCGACCACATATACGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGTCATCCTCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGc                                                                                                                                                                           <  1:75025/292‑1 (MQ=255)
                                                                                                                                     cATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGACAGGCGGGTCATTACTTTATCGATATACTTCCCCGTTTGCCTCCCCGGACCaa                                                                                                                                                              >  1:445887/1‑300 (MQ=255)
                                                                                                                                      aTTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAg                                                                                                                                                                                                                      >  2:413629/1‑243 (MQ=255)
                                                                                                                                                tcCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATCAAGGTAATATACAGCGCACCAACCAGGGTAAGGCGGGTCATTACTTTATCGATCTACTTCGCCGTTTGCTCTCCCGGACTACTTCCTGGTACa                                                                                                                                                   >  2:261210/1‑300 (MQ=255)
                                                                                                                                                             gggCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACtt                                                                                                                                                                                     >  1:302360/1‑253 (MQ=255)
                                                                                                                                                             gggCGACCAAGTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACtt                                                                                                                                                                                     <  2:302447/253‑1 (MQ=255)
                                                                                                                                                                            ggCCCTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATctgc                                                                                                                      <  2:104393/301‑1 (MQ=255)
                                                                                                                                                                              ccGTGGCCTTCTCGGTTCTCCTTCTTCAATGCAGACTCATACTTCCTGGACACATTCAGAGTTTGCACTTGAGCCATAAAATCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATctgctg                                                                                                                    <  1:135517/301‑1 (MQ=255)
                                                                                                                                                                                  aGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTc                                                                                                                >  2:134371/1‑301 (MQ=255)
                                                                                                                                                                                                     cttcAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACAAGGGTCAGGCGGGTCATTACTTGATCGATATACTTCGCCGTTTGCTCTCCCGGCCGAACTCCTGGTACACATGCACCGGCACTCTTCTGGTTACCTGCTTTGTCACTCgggagacaagacaac                                                                                             >  1:260395/1‑289 (MQ=255)
                                                                                                                                                                                                         agttcagacgtgtactcatacaaactcataaGAGTAATCACTTGAGCTAAAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTAACTGCTGTTTCACGCGGGTTGAAAACCAACGCCg                                                                                         <  2:132402/276‑1 (MQ=255)
                                                                                                                                                                                                                     aactgactgAACAACATCAGAGTTTGCACTGGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGTGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAAc                                                                             <  1:90317/300‑1 (MQ=255)
                                                                                                                                                                                                                      cctgaagggACATAATCAGAGTTTGGACGTCAGCCATAAAGTCCATAAGCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAAc                                                                             <  1:278572/293‑1 (MQ=255)
                                                                                                                                                                                                                             ggACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATTTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAGGAAACagaagaa                                                                      >  2:467219/1‑300 (MQ=255)
                                                                                                                                                                                                                                   catcaGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGTTTTACTTCGCCGTTTGCTCTCCCTGCCTAATTCCTGTTAAACAGGCACCGGACTTCTTTAGGTTATCTGCTGTTTCACGCGCGTTGATATCTAACGctgcgaccggactagcggcgttttggga                                                                >  1:132369/1‑273 (MQ=255)
                                                                                                                                                                                                                                               cACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAgcgc                                                                                                                                                                                                                               >  2:71251/1‑129 (MQ=255)
                                                                                                                                                                                                                                               cACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAgcgc                                                                                                                                                                                                                               <  1:71230/129‑1 (MQ=255)
                                                                                                                                                                                                                                                       ccACAAAGCACATAAGCACGACAACAACGATAAGCAGTGAGGGCCCACCGTAGTAGAACGGTACTATCATTGCATCACTCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                           <  2:62193/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                ccATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTACGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAGTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACAAACGCCGCGTAGAGGAACCAGAAGAGGATGATTGAAAACACATAGAAGAACACACAAATCgg                                  >  2:330607/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                  aTAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCCCGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGAAGACGCATAAAGTAAAACATAAACGGGtt                                >  2:212207/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                   acaacACGAACACAACGATAAGCACGGATGTCCCAACGGAGTATAACGGTACGTTCATTGCATCACGCATGAACTCCGGGATCAGTCAGATAAAGGTAAGAGACAGGGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTg                               <  2:362063/297‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGAAGACGCAAAGAGTAACAAAAAAAGCGGTTGCCAAGGCTGCAAATAc               >  1:327416/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                   caTAAGCAGTGCGGTCCCACCGTAGGAGAACGGTACTTTCATTGCATCACGCATGAACTCCAGGATCCGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATAc               <  1:134338/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      aaGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTTCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGc            <  2:446007/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                gTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGActtctt                                                                                                                                  <  2:102885/173‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                gTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGActtctt                                                                                                                                  >  1:102859/1‑173 (MQ=255)
                                                                                                                                                                                                                                                                                                gTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGCACAAATGCACCGGACTCCTTCAGGTTATCTGCTGTTTCCCGCGTGTTGAAAACCAACGCCGTGCAGACGAAACAGACGAAGATGATTGAAGACGCAGAGAGTAACACACAAACCGGTTGCCAAGGCTGGAAATACAGCGAAAttggtt  >  1:385417/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   
AGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTG  >  NC_000913/3442599‑3443187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A