Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F17 I0 R1 115 121.7 2441348 96.4% 2353459 231.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,782,918 T→C 34.8% D248G (GAT→GGT)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9180TC34.8% 55.5 / 7.8 56D248G (GAT→GGT) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base T (16/15);  new base C (4/12);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCA  >  NC_000913/3782688‑3783152
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
aTGCTGCCTCGCGGGTGTTTTTCCCGCATTATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCCCCTGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACAAATCTTCTCCTGCGCGTTTTGTCCACCCATGCCCTGACCG‑AGCATCATGCCAAACCGGCGG‑TTCCGCG‑CC‑TGGTTG‑TCGGTACAGGTAACCCCCAGATCGCCAAGc                                                                                                                                                                                                                                      <  2:975316/239‑1 (MQ=255)
 tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGGTG‑TCGGGACAGGGAAACACCAGATAGcccaac                                                                                                                                                                                                                                      >  1:883398/1‑234 (MQ=255)
      ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACCGG‑CC‑CCGTTT‑TCGGTGAAGGCAAACACCAGAACACCAAGACCCGc                                                                                                                                                                                                                                 >  2:327306/1‑238 (MQ=255)
       cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑GC‑TGGTTG‑TCCGCAAAGGGAAGCACCAAAACGCCGAGCCCCGcc                                                                                                                                                                                                                                >  1:471190/1‑238 (MQ=255)
       cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTAAGCG‑GC‑TGGTTG‑TCGGTACAGGGAAGCACCAAATCCCCAAGCcccccc                                                                                                                                                                                                                                >  1:688456/1‑238 (MQ=255)
                gTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTTCCGATAAGCACCAAACCGCCCAGCCCCGCCC‑TGCCCCTac                                                                                                                                                                                                                     >  1:608963/1‑238 (MQ=255)
                          cAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑CGGTTG‑TCGGGGGAGGCAAGCACCACACCGCGCAGCCCCGccccacccctcaaagtgggcag                                                                                                                                                                                                            >  2:831241/1‑219 (MQ=255)
                                  taCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTTCGCG‑AC‑TGGTTT‑TCGGTACACGGAAACACCAGAACGCCAAACCCCCCCC‑AGCCCAAAAAGGGGGcggaggcgtga                                                                                                                                                                                                    >  1:609440/1‑228 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAACCGGCGG‑TTTCGCC‑CCACGGTTG‑T‑GGCGCCGGAGATCACCACATCGCCAATGCCCGCCC‑TGCCCCTAAACGTGCCAGGGGCGGCcccccg                                                                                                                                                                                               >  2:921255/1‑236 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAAGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTT‑TCGGGACCGGGAAGCACCAAATCGCCAAACCCCGCCC‑TGCCCATAAAGGGGGGAGGGTCGGGAcccc                                                                                                                                                                                                >  1:11189/1‑237 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGGTTTACG‑G‑AC‑TGGTTG‑TCGGCACAGGTAAGCACCAGACCACCCAGCCCCCCCA‑GCCCCCTAAATGGGGCAGCGTGGGCAacccc                                                                                                                                                                                               >  2:598322/1‑236 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTTACCG‑GC‑CTGTTG‑TCCGTAAAGGGAAACACCAAAACCCCAAACCCCCCCACCGCCCTAAAAGGGGGCGGGGCGGGCACCCa                                                                                                                                                                                                >  2:1092939/1‑238 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑GC‑TGGTTG‑TCGGTACAGGTAAACACCAAATCGCCAAGCCCCCCCC‑TGCCCCTAAAAGGGGCAGGGGCGGCCCCCAgc                                                                                                                                                                                              >  1:68709/1‑239 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGGACAGGTAAGCACCAAATCGCCAAGCCCCCCCA‑CGCCCATAAAGGGGGGACGGGCGGGACCCAgc                                                                                                                                                                                              >  1:189451/1‑239 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGGTG‑TCGGGGCAGGGAAACAACAAATCCCCAAACCCCGCCA‑TGCCCCTAAAAGTGGCAGGGGCGCGAcccca                                                                                                                                                                                               >  1:967795/1‑236 (MQ=255)
                                        aTAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGGACAGGTAAGCACCACAACGCCAAACCCCGCCA‑TGCCCCTAAAAGGGGCAGGGGCGGCACCCacc                                                                                                                                                                                              >  1:405357/1‑236 (MQ=255)
                                         tAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTTCGCC‑CC‑TGGTTG‑TTGGGACAGGTAAGCACCAAATCGCCAAGCCCCGCCC‑TCCCCTCAAAGGCGGCACGGTCGGCACCCAccg                                                                                                                                                                                             >  2:63457/1‑238 (MQ=255)
                                         tAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGCGCAGGGAAACACCAGATCGCCAAGACCCGCCA‑TGCCCATAAAGGTGGCATGGTTGGATCCCacct                                                                                                                                                                                             >  1:320022/1‑235 (MQ=255)
                                                       tATGGGCATTTCAGCGCCGAAGCGTTGCGCCATTCCTCGGACTCCTTTCGTTTTGCGGTACCCTCCCACCCCCTGACCAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCTACATGCCAAACCGGCGG‑TTACGCG‑CC‑TGTTTT‑TCGGTACAGGTAGGCCCCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGAcgc                                                                                                                                                                              <  2:467317/238‑1 (MQ=255)
                                                                ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TTGGGACAGGTAAGCACCAAAACGCCAAGCCCCCCCC‑TGCCCCTAAAGGTGGCAGGGTCGGCACCCAACGCCGCA‑CCaaacagcgaccattca                                                                                                                                                                     >  1:109317/1‑224 (MQ=255)
                                                                ttCAACGCCGAAGCGATGCGCCAGTTCGAGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TCTCGGC‑AC‑GGGTTT‑TCTCTGCAGGGAAGCACCAAACCACCACGACCCGCCA‑TCCCCCCAGAGCGGGCAGGGGCCGCCACCAGCCGCCCC‑CCCAGAcgccgcacatca                                                                                                                                                                     >  2:555810/1‑229 (MQ=255)
                                                                 tCACCGCCGAGGCGATCCGCCAGTGCGCGGCCTTCTTCGTTATTGCGGTGGCCTTCCACCACCTGACCATTCTGCTCCGGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGGG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTcagc                                                                                                                                                                   <  2:703578/239‑1 (MQ=255)
                                                                 tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTcagc                                                                                                                                                                   <  1:1093905/239‑1 (MQ=255)
                                                                                             ggccttcttTCGTATTGCGGTAGCCTCCCACCACCTGACCAATCTCCTCCTGCGCGCTTTGTACACCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTGCGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTAcg                                                                                                                                        <  1:508833/235‑1 (MQ=255)
                                                                                                    tttCGTATTGCGGTAGCCTCCCCCCCCCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCg                                                                                                                                <  1:941838/239‑1 (MQ=255)
                                                                                                        gTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCAGGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc                                                                                                                            <  1:63457/239‑1 (MQ=255)
                                                                                                         tATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCGCAC‑TGG‑GG‑TCCGTCAAAGGAAGCACCAAAACGCCAAGCCCCGCCC‑TGCCCCTCAAGGGGGGAGGGGCGGGACCAAACGCCCCACCCAAGAC‑CGACAATTCAGACAGCCCCCAGGGGAGCAGCCCCGCACACCCCTTCGCCCCa                                                                                                                           >  2:1072251/1‑239 (MQ=255)
                                                                                                                 tctccTTCCCCCCCCTGACCAACCTTCCCCGGCGCGCTGTGTACATCCAGGCCCTGACCG‑GGCACAACCCCAAACCGGGGG‑TTCCGCG‑TC‑TGGTTG‑TGGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                    <  2:405357/235‑1 (MQ=255)
                                                                                                                          ccccccggACCAATCTTCTCCTGCGCGCGTTGTACACCCAGGCCCGGCCCG‑ACCATCAGGCCAAAACGGCGG‑TTCCGCG‑CG‑TGTTTG‑TCGGTACAGGTAAGCCCCAGCTCCCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                          <  2:1100751/232‑1 (MQ=255)
                                                                                                                          ccccccTGCCCAACCTCCCCCGGGGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAACCGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                          <  1:968163/235‑1 (MQ=255)
                                                                                                                          ccccccTGACAATGCTCCTCCGGCCCGCTTTGACCCTCCATCCCCGGACCA‑GCCATCAGGCCAAACCGGGGG‑TTCCGCG‑CC‑GGGTTG‑TGGGTACAGGTAAGCGCCAGACCCCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                          <  2:109317/235‑1 (MQ=255)
                                                                                                                                      aTCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCAAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCAcc                                                                                              <  1:231348/239‑1 (MQ=255)
                                                                                                                                                 cgcgcTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTATGCG‑GC‑CGGTTG‑TCGGTTCAGGGAAGCACCAGAAAGGCCAGCCCCGCCC‑TGCCCATAAAAGGGGGAGGGGCGGCACCCAGCGCCGCC‑CCCAAAAGCGCCAATTAAGGCAGCCCCCGGGTGAGAAGCGCCCGACGCGCACTCCGAACCAAACAGAACCCGTCTGAGATacccccccccagagcaacca                                                                                   >  2:71097/1‑223 (MQ=255)
                                                                                                                                                 cgcgcTTGGTACATCCAGGCCCTGACCG‑AGCATCAGGCCAAACCGGCGG‑TTACGCG‑AC‑GGGTTC‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATa                                                                                    <  1:527888/238‑1 (MQ=255)
                                                                                                                                                             aTCCCTGCCCGTACCG‑AGCCTTAGGCCAACACGCGGG‑TGAGGGG‑AC‑‑GGTTGCTCGTTACAGGTACGCACCGGATCCCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                       <  2:751799/239‑1 (MQ=255)
                                                                                                                                                             aTCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                       <  1:342028/239‑1 (MQ=255)
                                                                                                                                                             aTCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑CCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                       <  1:1092939/239‑1 (MQ=255)
                                                                                                                                                                ccagccccagcgcgcgagtcatGCAAAAACGGCGC‑TCCCGCG‑AT‑GGGTGG‑ACGTAACAGGTACGCACCACCCCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCCCCAATGGCAATAACGTTTTTCGCcgcg                                                                     <  2:549394/221‑1 (MQ=255)
                                                                                                                                                                          ccG‑AGCATCATGCCAAAGCGGCGG‑TTACGCG‑CC‑TGTTTG‑CCGGTACAGGTAAGCCCCAGACCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg                                                          <  1:495532/239‑1 (MQ=255)
                                                                                                                                                                             ggccatcagGCCAAACCGGCGG‑TTCCGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGACCCCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa                                                        <  1:595791/236‑1 (MQ=255)
                                                                                                                                                                                cgtaagcccAAACCGGCGT‑TTACGCG‑AC‑GGTTTG‑CCGGTACGGGAAAGCCCAAGACCGCCAAGCACCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa                                                        <  1:416768/229‑1 (MQ=255)
                                                                                                                                                                                     tGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCCCCAGAGCGCCAGGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATg                                                <  1:921255/239‑1 (MQ=255)
                                                                                                                                                                                              ggcgg‑TTCCGCG‑AC‑GGGTTG‑GCGGTGCAGGTAAGCCCCAGACCGACAGGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa                                       <  1:606922/239‑1 (MQ=255)
                                                                                                                                                                                                        gcg‑GC‑TGGTTG‑TCGTGACAGGTAAGCCCCAGATCGCCAACCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTaa                              <  1:598322/239‑1 (MQ=255)
                                                                                                                                                                                                                    tgccGGTGCAGGAAAGCACCAGACCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaa                      <  1:81510/235‑1 (MQ=255)
                                                                                                                                                                                                                               gcaaGCCCCAGACCCCCAACCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGACCCCAGCGCCGCA‑CCAAGCCGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAGCGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg            <  2:11189/235‑1 (MQ=255)
                                                                                                                                                                                                                                tAAGCCCCAGATCCCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg            <  1:1072251/236‑1 (MQ=255)
                                                                                                                                                                                                                                  aGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGt        >  1:517045/1‑238 (MQ=255)
                                                                                                                                                                                                                                    cacaAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                    <  1:1171738/128‑1 (MQ=255)
                                                                                                                                                                                                                                    cACCAGCTCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCATCGCCGTACGCGCATTCGCACCAAACCCGCTACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCCCGCCACTGAAATCCGGCTTGCTGTAAACCCGGAGACTTTTGCCGCAGTgca     >  2:388047/1‑239 (MQ=255)
                                                                                                                                                                                                                                    cACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                    >  2:1171738/1‑128 (MQ=255)
                                                                                                                                                                                                                                    cACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGCGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCCGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACCTCCCCGCACCAATGGCAATAACGTTATTCACCGCCCCGCCATCCTGCACGCCACTGAAATCCGGATTGCTGTAACCTCGGAAACTTTTGCCGCAGTgc      >  2:975882/1‑238 (MQ=255)
                                                                                                                                                                                                                                      ccAGACCGCCACGCCGCGCCA‑TGGCCCGAAAGGAGCCAGAGTCGGGTCTCAGCGCCGCA‑CCAAGACGCGCCATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAATCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCGGCCAAGCTGGACGCCGATGAAATCCGGATTGCTGTAAACGCGGAATCTTTTGCCGGAGTgcagc   <  2:250488/239‑1 (MQ=255)
                                                                                                                                                                                                                                         gATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcagca  >  1:226212/1‑237 (MQ=255)
                                                                                                                                                                                                                                         gATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGCACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGCTTGCTGTAACCGCGGACACTTTTGCCGCAGTgcggca  >  2:151646/1‑237 (MQ=255)
                                                                                                                                                                                                                                         gATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGACACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcagca  >  2:257281/1‑237 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
ATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCG‑AGCATCATGCCAAAACGGCGG‑TTACGCG‑AC‑TGGTTG‑TCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCA‑TGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCA‑CCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCA  >  NC_000913/3782688‑3783152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A