Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,159,550 T→G 15.6% T668P (ACC→CCC)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,5500TG15.6% 200.9 / 4.0 97T668P (ACC→CCC) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (43/34);  new base G (13/7);  total (56/41)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCAC  >  NC_000913/1159315‑1159778
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    
tGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAAAAGGTTATTGACGTTCCCCGGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCCGCGC‑GTGGCTACCTTGCCCGGCGGGGAAGGGGcc                                                                                                                                                                                                                                   >  2:775659/1‑239 (MQ=255)
tGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATAGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTACCGAGTAGTTTTTCGCCCCCTGGAAGCGGGTGAAGAGATCCACCAACGC‑GAACCTACCTTGCCCGGGGCGGAAGGGGcc                                                                                                                                                                                                                                   >  2:282631/1‑239 (MQ=255)
   ggAGGCTAAGGCCGCCAGATGGTTTCCTTTTTTACAGGTTTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGGGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATa                                                                                                                                                                                                                                <  2:889419/239‑1 (MQ=255)
       aCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTTGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGCGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGc                                                                                                                                                                                                                            >  1:836121/1‑239 (MQ=255)
           aggcaggcAGTTGGCTGTCTTTTTTCAAGGTGTTTTTCAGAATTGATGCGGGCCTGTAATGCTGAAATTACGGGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCCCCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCa                                                                                                                                                                                                                        <  2:8935/239‑1 (MQ=255)
           aGGCAGCCAGGTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATCCGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCa                                                                                                                                                                                                                        <  1:323392/239‑1 (MQ=255)
           aGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCa                                                                                                                                                                                                                        <  1:678647/239‑1 (MQ=255)
              cAGGCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACg                                                                                                                                                                                                                      <  1:421635/238‑1 (MQ=255)
                 ccAGCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATAGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGGAATCCACCAGCGC‑GTAGCTACCTTCCCCGGCGCGGAAGGGGCAATAAGGCGCCAgggagg                                                                                                                                                                                                                  >  2:672106/1‑236 (MQ=255)
                 ccAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGTCCATACGGCGTCACGgtgc                                                                                                                                                                                                                  >  1:1052111/1‑238 (MQ=255)
                 ccAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGGGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCCCGGCGCGGAAGGGGCCAAACGGCGCCACggggg                                                                                                                                                                                                                  >  2:779451/1‑236 (MQ=255)
                     aTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTCGACGATTGAAC‑CTTACACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGTCGTTCCCCTGCAACGAGAAGTTTTTCGCCACCTGGTCGCGGGTGAACAGTACCACCA‑CGCGGTAGCTACCTTTCTCGGTGCGGAACGGGCCAACCGGCGCCCCGGTTCCGGt                                                                                                                                                                                                              >  2:113065/1‑239 (MQ=255)
                           gCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGGGATCCACCAGCGC‑GTAGCTACCTTGCCCGGCCCGGAAGGTTCCAACAGGCGTCACCGAGTGGGtcaaca                                                                                                                                                                                                         >  2:169856/1‑233 (MQ=255)
                                       gtggtTTTTCAGAATTGATGCGTGCCGGTAATGCTGAAATTACGCGGTGGGCCGGAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTGGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCCCCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCa                                                                                                                                                                                            <  2:362013/236‑1 (MQ=255)
                                          gttttttgtaaTGGATACTGGTCGGTAATGGTGAAATTCCGGGGTGGTCCGGAGACGAT‑GAACTCTGCGCGGTGGGTATCGTAGGTTTTGTCAAAGAGGTTAGTGACGTGCCCCGGAAACGAGAAGTTTTTCGGCACGTGGTAGGGGGTGAAGAGACCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCTTCACGGTGTCGGTATACCCGCGATTTTGCCAGtt                                                                                                                                                                                         <  2:697038/230‑1 (MQ=255)
                                           ttATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTATCGGGTGACGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCGTCACGGTGTCGGTTTACCCCCGATTTTGCCAGTTa                                                                                                                                                                                        >  2:944737/1‑239 (MQ=255)
                                           ttATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACgg                                                                                                                                                                                                                     >  1:221154/1‑210 (MQ=255)
                                           ttATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACgg                                                                                                                                                                                                                     <  2:221154/210‑1 (MQ=255)
                                           ttATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGGGTCGGTATACACGCGATTTTGCCAGTTa                                                                                                                                                                                        >  1:520131/1‑239 (MQ=255)
                                                   aaTTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGCCGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTCGCGGGTGAAAAGATCCCCAAGCGC‑GTAGCTCCCTTGCTCGGCCCGGAAGGGGCCATACGCCGCACCGGGGTCGGTATACAACGGACTTTGCCAGTTAAaaacaca                                                                                                                                                                                >  2:311215/1‑238 (MQ=255)
                                                   aaTTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCAACGTTGGTATAGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAAcaccac                                                                                                                                                                                 >  1:440776/1‑238 (MQ=255)
                                                        aTACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGGTTTTGCCAGTTACCACCAcccccg                                                                                                                                                                            >  1:945573/1‑238 (MQ=255)
                                                        aTACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCAccgccg                                                                                                                                                                            >  1:955478/1‑238 (MQ=255)
                                                        aTACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCGTCACGGGGTCGGGATACACGCGATTTTGCCAGTTAACACCAcccccg                                                                                                                                                                            >  1:1025558/1‑238 (MQ=255)
                                                        aTACGTGCCGGTAATGCTGAAATTACGCGGGGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCGTCACGGGTTCGGTATAAACGCGATTTGGCCAGTTAACACCAccgcca                                                                                                                                                                            >  1:1003799/1‑237 (MQ=255)
                                                             tGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGACGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGGGCGGAAGGGTCCTAACGGCGTCCCGTGGTCGGTGTACCCTCGATTTTGCCAGTTTAcaccaacccccccccgc                                                                                                                                                                      >  2:739956/1‑227 (MQ=255)
                                                             tGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTAGAACAGGTTATTGACGTTCCCCTGTAAGGAGAAGTTTTTCGTCACCTGGTAGCGGGAGAAGAGAGCCACCAGCGG‑GAAGGTACCTTGTTCGGCGCGGAAGGTGCCATCAGGAGTCACGGGGCCGGAGTACACGCGATTTTGCCAGGTaaagacaaccccgacgggg                                                                                                                                                                      >  2:572205/1‑222 (MQ=255)
                                                                        cTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGgtgt                                                                                                                                                                                                                  >  1:400152/1‑184 (MQ=255)
                                                                        cTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGgtgt                                                                                                                                                                                                                  <  2:400152/184‑1 (MQ=255)
                                                                          gAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGGTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGTCGCGGAAGGTGCCATACGGCGCCACGGGGTCGGTATAACGGCGATTTTGCCACTTAAACCAACCGCCGCCCGTCCACCCTGGCAAGa                                                                                                                                                         >  2:722648/1‑239 (MQ=255)
                                                                           aaaTTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGCCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGAc                                                                                                                                                        >  1:82946/1‑239 (MQ=255)
                                                                              ttACGCGGTGTGGCGTAGACGATAGACC‑CTTCCCCGTTGGTATCGTAGGTTTTGTCGAACAGGTTATGGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAgg                                                                                                                                                     <  2:1025558/239‑1 (MQ=255)
                                                                                tggcgGTGGGCCGTAAGCGATAGAAC‑CTTCCTCGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAgg                                                                                                                                                     <  1:906263/235‑1 (MQ=255)
                                                                                        tgCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCCTGACAGGCAACCGATa                                                                                                                                            >  1:622691/1‑238 (MQ=255)
                                                                                        tgCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACAACCGCCGACCGTCAACCCTGGCATGCCAGGCAACCGATa                                                                                                                                            >  1:579784/1‑238 (MQ=255)
                                                                                              tatgtgttgcaC‑CTTGCGCGTTGGTTTCGTTGGTTTTGTCGAACTGGTTTTTGACGTTCCCCGTGAACGGAAAGTTTGTCGTCCCGTGGTAGGGGGGTAAGAAATCCACCCGCGC‑GTAGCTACCTTGCGCGGGGCGGAAGGTGCCATCCGGCGTCACGGTGTCGGTTTACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTg                                                                                                                                     <  2:476254/229‑1 (MQ=255)
                                                                                              tagctgttacaC‑CTTCCCCTTTGGTTTGGTGGGTTTTTGCCAACAGGTTTGTGACTTTCCCCTGTAACGGGAAGTTTTTCGTCGCCTGGTTGCGGGTGAAGAGATCCCCCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCCCGGTGTCGGTATCCACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTg                                                                                                                                     <  2:436714/229‑1 (MQ=255)
                                                                                              aggcgtatgAAC‑CTTCCACGTTTGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTg                                                                                                                                     <  1:775659/231‑1 (MQ=255)
                                                                                               aaCGTTAGAAC‑CTTCCACTTTGGTTTCTTAGGTTTTGGCGACCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCCCCTGGTAGCGGGTGAAGAGACCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATCCGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTg                                                                                                                                     <  1:1020381/237‑1 (MQ=255)
                                                                                                aCGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGGGaa                                                                                                                                   >  1:436714/1‑239 (MQ=255)
                                                                                                aCGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGCCGTCACGGGGTCGGTATACACGCGATTTTGCCAGTTCACACCACCGCCGACCGTCCACTCTGGCCGGACAGGCAAACGATAGCCGGCGaa                                                                                                                                   >  2:31054/1‑239 (MQ=255)
                                                                                                  gATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGCTACCTGGGGaa                                                                                                                                   >  1:819881/1‑237 (MQ=255)
                                                                                                    tAGAAC‑CTTCCACGTTTGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACAt                                                                                                                                <  1:1065531/238‑1 (MQ=255)
                                                                                                    tAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGCCCGTCAACTCTGGCATGACAGGCAACCGATAGCTGTGGAACAtt                                                                                                                               >  1:938883/1‑239 (MQ=255)
                                                                                                         cctgtCCAAGTTGGTTTCGTAGGTTTGCGCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTGCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGAGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTaa                                                                                                                           <  1:1021317/235‑1 (MQ=255)
                                                                                                            ttccggtttGTTATCGTGGGTTTTGTGGAACATGGTTTTGACGTGGCCCTTTAACGAGAAGTGTTTCGCTCCGTGGTAGCGGGTGAAGAGAGCCACCAGCGG‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCg                                                                                                                        <  2:29752/232‑1 (MQ=255)
                                                                                                             tCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTAACGGGGTCGGTATACACGCGATTTTGCCAGTTAACACAACCGCCGACCGTCAACTCTGGCCTTACAGGCAACCGCTAGCTGGGGAACATTTTAAACgt                                                                                                                       >  1:442223/1‑239 (MQ=255)
                                                                                                             tCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGACGCGGAAGGTGCCATCCGGCGTCCCGGGGTCGGTATACACGCGCTTTTCCCAGTTAACACCACCGCCGACCGGCAACCCTGGCATGAAAGGCAACCGAAACCTGGTGAACATTTTAACCgt                                                                                                                       >  2:259023/1‑239 (MQ=255)
                                                                                                               cACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTTTACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCCAGAACGGCAACCGCTAGCCGGGGAACATTTTAACCctgg                                                                                                                     >  2:966843/1‑239 (MQ=255)
                                                                                                                              ggTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGCCAACCCTGGCATGACAGGCAACCGCAACCTGGTGAACATTTTAACCGGGGGGCGTGGCAGATAAg                                                                                                      >  2:638977/1‑239 (MQ=255)
                                                                                                                                ttttGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGGGCGTGGCCGATTAGGa                                                                                                    >  1:612432/1‑239 (MQ=255)
                                                                                                                                ttttGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCCTCAACTCTGGCATGACAGGCAACCGATAACTGGGGAACATTTTAAcctggggacggggggaaatagga                                                                                                    >  2:1036160/1‑218 (MQ=255)
                                                                                                                                 tttGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAAAACCACCGCCGCCCGTCAACTCTGGCATGAAAGGCAACCGATAGCTGGGGAAAATTTTAACCGTGGGGGGGGGAAGATTAGGAt                                                                                                   >  2:403539/1‑239 (MQ=255)
                                                                                                                                     tCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAAc                                                                                               >  1:819282/1‑239 (MQ=255)
                                                                                                                                               tttggACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCt                                                                                     <  1:403539/235‑1 (MQ=255)
                                                                                                                                                   gACGTTACCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGCTTTTGCACGTTACAACCACCGCCGCCCGTCCACTCTGGCAGGAACTGCAACCGATAGCCTGTGAAAATTTTAACCGTGGTGCGGGCCAGATGCGGGTTAACGGCGTTTCAttttt                                                                                 >  2:573835/1‑236 (MQ=255)
                                                                                                                                                       ttCCCCTTTAACGGGAATTTTTTGGTAACCTGGTTGGGGGTGAAGGACTCCGCCGCGGC‑GTAGCTACCTTGCGCGGCGCGGAAGGGTGCATACGGGGTCACGGTGTCGGTAAACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGAGAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATc                                                                             <  2:442223/239‑1 (MQ=255)
                                                                                                                                                       ttCCCCTGTACCGGAAGGTTTTTCGTCCCTTGGGGGGGGGTGAAGAGAGCCCCCAGCGC‑GTAGCTCCCTTGCTCGGCGCGGAAGGTGCCATCCGGCGCCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATc                                                                             <  2:628720/239‑1 (MQ=255)
                                                                                                                                                       ttCCCCTGTAACGTGAAGTTTTTCGTCCCCTGGGTGGGGGTGAAGAGTTCCCCCAGCGC‑GTAGCTACCTTGCCCGGCGCGGAAGGTGCCATGCGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATc                                                                             <  2:1052111/239‑1 (MQ=255)
                                                                                                                                                       ttCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGAAAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGGGCGTGGCAGATTAGGGTTAACGGCGTTTCCTTGGTTAt                                                                              >  2:589822/1‑238 (MQ=255)
                                                                                                                                                       ttCCCCTGAAAGGGGAATTTTTTCCTCCCTTGGTAGGGGGGGAAGAGATCCACCAGCGC‑GTAGCTACCCTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATc                                                                             <  2:622691/239‑1 (MQ=255)
                                                                                                                                                        tCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATc                                                                             <  1:638977/238‑1 (MQ=255)
                                                                                                                                                         ccccTTTAACGAGAATTTGTTCGTCACCTGGTAGCGGGTGAAGAGATCCCCCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCct                                                                           <  1:909835/239‑1 (MQ=255)
                                                                                                                                                            cttggaCGAAAATTTTTGCGGCCCCTGGGAGGGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACGCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTg                                                                        <  2:819282/234‑1 (MQ=255)
                                                                                                                                                               tAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCaa                                                                     >  1:192979/1‑239 (MQ=255)
                                                                                                                                                               tAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCaa                                                                     >  1:257283/1‑239 (MQ=255)
                                                                                                                                                                  cGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAatat                                                                  >  1:810336/1‑239 (MQ=255)
                                                                                                                                                                    agaAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCAGGACAGGCAACCGATAGCTGGTGAACCTTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGAAATAAAg                                                                >  2:751090/1‑239 (MQ=255)
                                                                                                                                                                      aaGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAAc                                                                                               <  1:162026/206‑1 (MQ=255)
                                                                                                                                                                      aaGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAAc                                                                                               >  2:162026/1‑206 (MQ=255)
                                                                                                                                                                           tttCGGCCCCTGGGGGCGGGTGAAGGCACCCCCAGGCGC‑GTAGCTACCTTCCCGGGGGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGGGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTg                                                         <  2:152696/239‑1 (MQ=255)
                                                                                                                                                                                     tggtggCGGGTAAAGAGTCCCCCCAGGGC‑GTAGCTCCCTTGCTCGGCGCGGAAGGTGCCATACGCCGTCACGGTGTCGGTATACCCGCGATTTTGCCAGTTAACCCCCCCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGt                                               <  2:836121/239‑1 (MQ=255)
                                                                                                                                                                                       gtgtggtgggaaggaaTCCACAGGCGC‑GTAGCTACCTTGCTCGGCGGGGGAGGGGACATACGGAGTCCCGGGTTAGGTATACGCGCGATTTTGACAGTTAACACCACCGCCGACGGTCAACTCTGGCATGACAGGCAACCGATAGCGGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCa                                             <  2:651890/224‑1 (MQ=255)
                                                                                                                                                                                        tAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAAc                                                                                                                                                                   >  2:43302/1‑120 (MQ=255)
                                                                                                                                                                                        tAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAAc                                                                                                                                                                   <  1:43302/120‑1 (MQ=255)
                                                                                                                                                                                             ggTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGTTGCGTGGCAGATTAGGATTACCGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGtacgcggcc                                       >  2:838851/1‑231 (MQ=255)
                                                                                                                                                                                              gTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGCTAGCTGGCGACCATTTTAACCGTGGTGCGTGGCAGATTCGGATTAACGGCGTTTCCTCCGTTTTCCTCTGCACAAAACCGGGTTGGGCCAAAGGCAAGCGGCCa                                      >  2:492321/1‑239 (MQ=255)
                                                                                                                                                                                                gAAGAGCTCCCCCAGCGC‑GTAGCTACCTTGCTCGGCGGGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAg                                     <  1:589822/238‑1 (MQ=255)
                                                                                                                                                                                                       tCCCCCAGCGC‑GGAGCTACCTTGCGCGGCGCGGAAGGTGCCATCCGGCGTCCCGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATc                                                                             <  2:971391/191‑1 (MQ=255)
                                                                                                                                                                                                        ccaccaGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGCGACCATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTGTCGCCAAATGTCAGCGGCCAGTTGTCggaa                            >  2:628211/1‑237 (MQ=255)
                                                                                                                                                                                                        ccaccaGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGGGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGCCCGTCAACTCTGGCATGACAGGCAACCGATACCCGGTGACCTTTTTAACCTTGGTGCGTGGCAGGTTCGGATTAACGGCGTTTTCCTCTTTATCCTCTGCAATATTCCGCGTGGCGCCAAGTGTACTCTGCCTGttccggcaa                            >  2:108515/1‑232 (MQ=255)
                                                                                                                                                                                                          ccccaggctgttgcctccTGGCCCGGCGGGGAGGGGTCCATCCCGAGTCAGGGGTTCGGTTTACAGGCGATTTTGCCAGTTAACGCCCCCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTaa                           <  2:440776/223‑1 (MQ=255)
                                                                                                                                                                                                              gcgc‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTgc                       >  1:111127/1‑238 (MQ=255)
                                                                                                                                                                                                               cgc‑gTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCg                                                                                                                                               >  1:38546/1‑117 (MQ=255)
                                                                                                                                                                                                                gc‑gTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCct                                                                           <  2:29598/184‑1 (MQ=255)
                                                                                                                                                                                                                gc‑gTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCct                                                                           >  1:29598/1‑184 (MQ=255)
                                                                                                                                                                                                                 c‑gTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAata                                                                   >  1:971391/1‑191 (MQ=255)
                                                                                                                                                                                                                     aacttccTTGCCCGGCGCGGAAGGTGCCATACGGCGTCACGGGTTCGGTATACACGCGATTTTGCCAGTTACCCCCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCGGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTg                <  1:672106/234‑1 (MQ=255)
                                                                                                                                                                                                                     aGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTAAGCTGCCAGTTGTCGGTAATTGTGCCGTTg                >  1:767165/1‑239 (MQ=255)
                                                                                                                                                                                                                     aGCTACCTTGCACGGCGCGGAAGGTGCCAGAGGGCGTAACGGTGTCGGTATACACGCGATTTTGCCAGTAAACCCCCACGGCGACCGTCAACTCTGGCATGGCAGGCAACCGATAGCTGGTTAACATTGTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTCCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGtt                 <  1:282631/238‑1 (MQ=255)
                                                                                                                                                                                                                               cTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAATTGTCAGCTGCCAGTTGTCGGTAATTTCGCCGTTGGGTCCAAAt       >  1:549468/1‑238 (MQ=255)
                                                                                                                                                                                                                                ccgggcgggAAGGTGCCATGCGGCCGCCCGGGGCCGGATTCCACGCTATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTc     <  2:192979/232‑1 (MQ=255)
                                                                                                                                                                                                                                  ggggcgGAAGGGGCCATACGGCCTCACGGTGTCGGTATAAGCGGGATTTTGCCAGTTAACCCCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCa   <  1:311215/236‑1 (MQ=255)
                                                                                                                                                                                                                                  ggCGGGGAAGGTGCCATACGGGGTCACGGGGTCGGTATCCACGCGATTTTGCCAGTTAACCCCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCa   <  2:819881/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ggCGCGGAAGGGGCCATACGGCGTCACGGTGTCGGTATCCCCGCGATTTTGCCAGTTAACCCCCCCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCa   <  2:157009/239‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcgGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTACCGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCGGCCATTTGTCGGGAATTGCCCCTTTGAGTTCAAATTCCAc  >  2:292502/1‑238 (MQ=255)
                                                                                                                                                                                                                                    cgcgGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCAc  >  1:157009/1‑238 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    
TGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC‑CTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGC‑GTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGCAACCGATAGCTGGTGAACATTTTAACCGTGGTGCGTGGCAGATTAGGATTAACGGCGTTTCCTTCGTTATCCTCTGCAATATAGCGCGTTGCGCCAAATGTCAGCTGCCAGTTGTCGGTAATTGCGCCGTTGAGTTCAAATTCCAC  >  NC_000913/1159315‑1159778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A