Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R1
|
19 |
52.5 |
1051376 |
98.5% |
1035605 |
229.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,478,306 |
A→T |
22.9% |
L27F (TTA→TTT) |
ynbB → |
putative CDP‑diglyceride synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,478,306 | 0 | A | T | 22.9%
| 69.0
/ 2.2
| 32 | L27F (TTA→TTT) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base A (2/22); new base T (5/2); total (8/24) |
| Fisher's exact test for biased strand distribution p-value = 2.27e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 |
GCGTTAATATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGC > NC_000913/1478074‑1478538
|
gcgTTAATATTTGGTCTGTGTGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGga‑g < 2:32390/239‑1 (MQ=255)
gcgTTAATATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGga‑g < 2:377389/239‑1 (MQ=255)
ttAATATTTGGTCTGTGGGGTCTGTCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGAATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATGTAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGACACGCTGATTAACCGTTTTCTGCTATGGCGGATGTCGgg‑gaga > 1:332419/1‑239 (MQ=255)
tatTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCGCAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAg < 1:276173/239‑1 (MQ=255)
tGGTCTGTGGGGTCTGTCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGACGGTTTCCGGA‑GAGGAAAggggg > 2:512077/1‑239 (MQ=255)
tGGGCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAggggg < 2:241863/239‑1 (MQ=255)
gTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACa < 1:93943/238‑1 (MQ=255)
gTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACa < 2:280407/238‑1 (MQ=255)
cTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTa < 1:9972/239‑1 (MQ=255)
tATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGG‑GAGAAAAGGGGGGGAGGTTTTCTTTTCGT‑TTTGTACCTGGTGGGGGAACGT‑CATTTTGttttttctggttg > 1:289155/1‑231 (MQ=255)
ttCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACg < 1:253976/239‑1 (MQ=255)
cAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGAGGGGAAAAGGGGGGGGGG‑TTAATTTCCGAATTGTTACTTGG‑GGGGGGTCGTCCT‑TTTGttttttattgtggttttatggtcaccag > 2:284846/1‑216 (MQ=255)
cTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGA‑GGGGAAAAGGGGTGAGG‑GTACATTACGTATTTCTACCTGGTGGGGGATCGT‑CC‑TTTTTTTTTTTATTGGGATTTTTGGTCCACGCTTGGtgacg > 2:20444/1‑239 (MQ=255)
tctcTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacgtt < 2:434278/239‑1 (MQ=255)
ctctGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAggggg < 1:343048/154‑1 (MQ=255)
ttAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcg < 2:292712/238‑1 (MQ=255)
tCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcg < 1:30440/228‑1 (MQ=255)
ttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTGTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATGCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa < 2:56452/239‑1 (MQ=255)
tcttATGAGCGCTGAAATATAATGCTGAACAAATCTCTGGCAACTCGTTTCGTGTTGTTAAGTTGAGCAACGCGGATAAACCGTTTTCTTCTATGGCGTTTTCCGGA‑GAGAAATGGGGTTGAGG‑TTACATTACGTTTTCGTACCTGGTGGCGCATCGT‑CA‑TTTGTTTTGCAATGGTGATTTCAGGTCCACGCTGGAAGACTTTGACGTTTTTTGCGCTGATAAGTTTTCTGTCATTGaa < 1:270722/239‑1 (MQ=255)
gcTGAAATATAATGCTGGAAAAATCGCTGGCAACTCGTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt < 1:222355/239‑1 (MQ=255)
tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt < 1:380250/232‑1 (MQ=255)
ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACGGA‑GAGGAAAGGGGGGGAGG‑GTTCATTACGTATTTGTACCTGGGGGGGGATCGT‑CC‑TTTGTTTTTTTATTGGGATTTTAGGTGCACGCTGGGTGACGGTGGCGGTTTTTTTGCTGTTAAGTTTTTTTGCATTGTAAGAAAATTGTAAGCTTTTATCTGTaa > 1:292521/1‑238 (MQ=255)
gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GGGAAAAGGGGGGGAGG‑GTTCATTACGTATTCGTATCCGGGGGGGGATCGT‑CC‑TTTTTTTTTTCATTGGGAGTTCCTGTCCCCGCTGGGTGCCGTGTTCGTTTTTTTTGCTGCTAAGTTTTTTTGCATTGGCAGAAAATTTTTCGCTtttaatctttct > 2:380250/1‑231 (MQ=255)
aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg < 1:59143/238‑1 (MQ=255)
aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg < 2:339430/238‑1 (MQ=255)
aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg < 1:20444/238‑1 (MQ=255)
ttttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTatat < 1:94101/239‑1 (MQ=255)
gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTGCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTa < 1:387866/239‑1 (MQ=255)
tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTAtt < 2:249963/239‑1 (MQ=255)
cAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGC‑GAGAAAAAGGGGGGGGG‑GTATCTTTCGTATTTGTCCCTTGTGGGGCATCGT‑CA‑TTTTTTTTTTTATGGTGGTTTCAGTGCCTCGCCGGGTGACGGTTGTGTTTTTTGTGCTGATCAGTTTTTTGGCATTTAAAGAATATTTTTAGCTTTTCTCTGTTCATTTTTTGCGTTGGGTTTTTTTTGGTCTTCCTCTTtattat > 2:251364/1‑239 (MQ=255)
gATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTa < 2:289155/239‑1 (MQ=255)
cGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGc < 2:292521/238‑1 (MQ=255)
|
GCGTTAATATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGC > NC_000913/1478074‑1478538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
