breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 433,649 T→G 29.9% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
RA 1,196,305 C→T 100% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,478,306 A→T 22.9% L27F (TTA→TTT ynbB → putative CDP‑diglyceride synthase
RA 1,865,874 T→C 10.4% E200G (GAG→GGG)  mipA ← scaffolding protein for murein synthesizing machinery
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 3,206,436 G→A 29.3% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,481 G→A 31.5% K7K (AAG→AAA ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,482 C→A 21.4% Q8K (CAA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,760,383 A→C 29.8% V289G (GTG→GGG)  selA ← selenocysteine synthase
RA 3,782,916 G→A 22.8% L249L (CTG→TTG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,862,672 G→A 43.7% pseudogene (931/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
MC JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,267 T→C 14.7% intergenic (+474/+170) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,568,566 T→A 17.2% intergenic (‑46/‑433) mdtM ← / → yjiP multidrug efflux system protein/pseudogene, transposase_31 family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1209603 1209618 16 39 [0] [0] 47 stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* * ÷ NC_000913 3423880–3424230 3424417–3424236 7–538 9 [8] [7] 9 rrlD 23S ribosomal RNA of rrnD operon
* * ÷ NC_000913 3769633 3769903 271 9 [8] [8] 9 [yibV]–yibV [yibV], yibV
* * ÷ NC_000913 4233768 4235397 1630 11 [7] [8] 9 pgi glucosephosphate isomerase
* * ÷ NC_000913 4476841 4477094 254 9 [8] [8] 10 yjgL SopA‑central‑domain‑like hexapeptide repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)54 (1.110) 43/438 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 458787 =8 (0.160)44 (0.920) 33/432 NT 84.8% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 608011 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? NC_000913 = 4587998 (0.160)36 (0.750) 29/430 NT 82.1% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 = 609347 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? NC_000913 458873 =10 (0.210)11 (0.230) 10/438 NT 52.4% intergenic (+173/‑15) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 = 575785 NA (NA)intergenic (‑32/+1) insH1/nmpC IS5 transposase and trans‑activator/DLP12 prophage, truncated outer membrane porin (pseudogene),IS, phage, Tn, Phage or Prophage Related, outer membrane porin protein, locus of qsr prophage
* ? NC_000913 = 45887712 (0.250)12 (0.250) 9/436 NT 50.1% intergenic (+177/‑11) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 574592 = NA (NA)intergenic (‑6/+145) nmpC/insH1 DLP12 prophage, truncated outer membrane porin (pseudogene),IS, phage, Tn, Phage or Prophage Related, outer membrane porin protein, locus of qsr prophage/IS5 transposase and trans‑activator
* ? NC_000913 1207790 =8 (0.160)40 (0.890) 32/406 NT 91.5% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 0 (0.000)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 12078058 (0.160)33 (0.730) 29/406 NT 89.9% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 0 (0.000)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =1 (0.020)65 (1.340) 51/438 NT 98.5% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)19 (0.390) 16/438 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)40 (0.820) 34/438 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2288917 =NA (NA)8 (0.160) 8/438 NT 23.5% intergenic (‑5/+146) yejO/insH1 pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system/IS5 transposase and trans‑activator
?NC_000913 = 3836841 26 (0.540)intergenic (+286/‑111) yicT/setC pseudogene/putative arabinose efflux transporter
* ? NC_000913 = 303240054 (1.110)3 (0.060) 3/424 NT 5.5% coding (1036/1449 nt) uacT uric acid permease
?NC_000913 = 3032403 50 (1.060)coding (1039/1449 nt) uacT uric acid permease
* ? NC_000913 = 3653229NA (NA)22 (0.450) 20/438 NT 56.4% intergenic (‑32/‑1) insH1/yhiS IS5 transposase and trans‑activator/pseudogene
?NC_000913 3836838 = 17 (0.350)intergenic (+283/‑114) yicT/setC pseudogene/putative arabinose efflux transporter