Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,949,064 T→C 14.8% E302G (GAG→GGG)  yifB ← magnesium chelatase family protein and putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,949,0640TC14.8% 333.9 / 12.1 145E302G (GAG→GGG) yifBmagnesium chelatase family protein and putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (69/51);  new base C (4/19);  total (73/72)
Fisher's exact test for biased strand distribution p-value = 4.78e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

TCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAATACGGCTGGCGAGC  >  NC_000913/3948830‑3949294
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          
tCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCtcatc                                                                                                                                                                                                                                        >  1:703200/1‑239 (MQ=255)
         tCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCCAATTCAGTAAGCTCATCAAGaaaaa                                                                                                                                                                                                                                >  1:642666/1‑238 (MQ=255)
         tCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑GTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCACGAAAAAg                                                                                                                                                                                                                               >  1:613233/1‑239 (MQ=255)
           gagaAAGGGCCCCGGGGGCCGGTTGAGATAACGTAATGTCTGTTCTGGTGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCa                                                                                                                                                                                                                             <  2:619997/239‑1 (MQ=255)
              aaggggCCCGGGGGGGCGGTTGAGATAACGTTATGTCTGTTCGGGGGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACg                                                                                                                                                                                                                           <  2:920510/235‑1 (MQ=255)
                  ggCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCGGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGGGTACGCCGTTCAAATTCCGGTAGCTCATCAAGAAAAAGCACGCCGtt                                                                                                                                                                                                                      >  1:754989/1‑239 (MQ=255)
                   gCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTa                                                                                                                                                                                                                     >  1:393866/1‑239 (MQ=255)
                    ccccGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCCCGC‑AAGGCATCCAGGGTACGCCGTCTCACTTCAGGAGGTCCACCAAGAAAAAGACCGCCGTTAt                                                                                                                                                                                                                    >  2:655081/1‑239 (MQ=255)
                            gCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGc                                                                                                                                                                                                            >  1:1230833/1‑239 (MQ=255)
                                       tAACGTAATGTCTGGTCTGGCGTTCAGGGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGGTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CAcc                                                                                                                                                                                                <  1:1017358/239‑1 (MQ=255)
                                         aCGTAATGTCTGTTCTGGCGTTCAGCGGTTATGGTTTCCCTGATAAGGTCCGGTAGGGCTGGAATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑TGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCtcatc                                                                                                                                                                                                                                        <  1:355241/198‑1 (MQ=255)
                                               tGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCGTCCAGTGTACGCCGTTCCAATTCAGGTAGCTCATCAAGAAAAAGAACGCCGTAATGCGCCACCGAAATTA‑CACCGGGCACTg                                                                                                                                                                                        >  2:1063131/1‑239 (MQ=255)
                                               tGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTg                                                                                                                                                                                        >  1:1174168/1‑239 (MQ=255)
                                               tGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTg                                                                                                                                                                                        >  1:850985/1‑239 (MQ=255)
                                               tGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTAATCAAGAAAAAGCCCGCCGTTATGCGCCGGGGAAATTT‑CACCGGGCCCTg                                                                                                                                                                                        >  1:804309/1‑239 (MQ=255)
                                               tGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGACCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCCCATCACGAAAAACAACGCCGTTATGCCCCAGCGAAATTT‑CACCGGGCCCTg                                                                                                                                                                                        >  1:616244/1‑239 (MQ=255)
                                               gacaggTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAAGGGCCGGTAGGGCTGGGATTCCTCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGccc                                                                                                                                                                                          <  2:1154765/232‑1 (MQ=255)
                                                gTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCCCCAGCGCAATTT‑Ccccgggcccggg                                                                                                                                                                                       >  1:291937/1‑239 (MQ=255)
                                                gTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGAACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCCCAATTT‑CACCGGGCCCCgg                                                                                                                                                                                       >  2:1311769/1‑239 (MQ=255)
                                                    gTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCACCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑AACCCGGCCCCGGaaat                                                                                                                                                                                   >  2:237130/1‑237 (MQ=255)
                                                     ttCTGGCGTGCAGCGGTTATGGTTTCCCTGATAGTGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGCTCTGCCC‑GGATTCAACCGGCTCTCGC‑AAGGCATCCGGTGTACGCCGTTCAAATTCAGACAGCTCACCACAAAAAACACCGCCTGTATGGCCCAGCGAAATTT‑AACCGGGCCGCGGaaaac                                                                                                                                                                                  >  2:376738/1‑236 (MQ=255)
                                                     ttCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAAtt                                                                                                                                                                                   >  1:528467/1‑238 (MQ=255)
                                                          gCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTTCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑CAGGCATCCAGTGTACGCCGTTCAAATTCAGGCAGCCTATCAAGAAAAAGACCCCCGGTCAGGCCCAGCGCACTTT‑CACCGGCCCCCCGAAttaggcc                                                                                                                                                                              >  2:1041316/1‑233 (MQ=255)
                                                          gCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGcc                                                                                                                                                                              >  1:297719/1‑238 (MQ=255)
                                                           gtggcgggggTTTTGTTTTCCTTGATAATGTCCGGTTGGGCTGGGATTCGTCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCa                                                                                                                                                                             <  2:431327/230‑1 (MQ=255)
                                                           ggttttgtataagaagccgcccTGTTAGTGGCCGGTAGGGCTGGGATTCATCGCCCAAACAAGGTGGAAGCGGGGTGGATAGG‑TTATTTTTG‑CTCGGGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCa                                                                                                                                                                             <  2:1370868/219‑1 (MQ=255)
                                                             tGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGAAGCTCATCAAGAAAAAGAACGCCGTTATTCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGGGCCAcc                                                                                                                                                                           >  1:915658/1‑238 (MQ=255)
                                                               cAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGGGcctgtc                                                                                                                                                                          >  1:1154765/1‑233 (MQ=255)
                                                                    gTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCCTCCAGTGTCCGCCGTTCAAATTACGGGAGCTCATCAAGACAACGCACGCGGTTATGCGCCAGGGAAATTT‑CCCCGGGCCCGGGGATGGCGCCCCCGCCCAccc                                                                                                                                                                   >  2:818361/1‑238 (MQ=255)
                                                                    gTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCCGTAGGTTCATCAAAAACAAGAACCCCGTTAGCGGCCACCCAAATTT‑CCCCGGGCCCTGGACATCCGCCCCCCCCCAcc                                                                                                                                                                    >  2:903711/1‑238 (MQ=255)
                                                                     ttATGGTTTCCCTGGTAATGGCCGGTAGGGCTGGGATTCATCCCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTGTG‑CCCGTGTGCGTT‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCa                                                                                                                                                                   <  2:297719/238‑1 (MQ=255)
                                                                           tttCCCTGATAATGTCCGGTGGGTTTGGTATTCACCCGCGAAAAAAGGTGGAAACGGGCTGGATAGG‑TTATTGTTG‑CCCGTGTGCGGG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGGGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGt                                                                                                                                                            <  2:278921/239‑1 (MQ=255)
                                                                             caggggtgtaaaaagaacaGGTCTGGGATTCACCGCCGCAGCAAGCGGGAAACGGGTTGGATTGG‑TTATTTTTG‑CTCGTGTGCTTG‑AAAGATGGACCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTa                                                                                                                                                          <  2:1197924/221‑1 (MQ=255)
                                                                              cccTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCCCCGTTa                                                                                                                                                          >  1:1068095/1‑238 (MQ=255)
                                                                               ccTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGCAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCCCGCCGTTATGCTCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCCCCGCCTCCCATCGCAGTTaa                                                                                                                                                         >  1:57478/1‑238 (MQ=255)
                                                                               ccTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCCCCCCCAACCaccccgtttaac                                                                                                                                                        >  2:334943/1‑228 (MQ=255)
                                                                                cTGATAATGTCGGGTAGGGCTGGTATTCATCGCCGCAACAAGCTGGAAACGGGTTGGATAGT‑TTATTTTGT‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACg                                                                                                                                                       <  1:237103/239‑1 (MQ=255)
                                                                                cTGATAATGTCCGGTAGGGCTGGGATTCAGCCTCGCAACAAGCTGGAACCGGGCTGGATAGT‑TTATTTTTG‑CTCGTGTTCGTT‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTTCGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACg                                                                                                                                                       <  1:818361/239‑1 (MQ=255)
                                                                                cTGATAATGTCCGGGAGGGCTGGGATTCACCGCCGCAACAAGGTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGGGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACg                                                                                                                                                       <  2:1093334/239‑1 (MQ=255)
                                                                                cTGAAAATGGCCGGTAGGGTTGGGATTCCTCGCCGCAACAAGCTGGAAGCGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGGGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACg                                                                                                                                                       <  1:581899/239‑1 (MQ=255)
                                                                                   aTAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCtca                                                                                                                                                                                                                                          >  2:60212/1‑154 (MQ=255)
                                                                                   aTAATGGCCGGTAGGGCTGGGATTCATGGCCCCAATAAGCTGGAAACGGGCTGGATAGG‑TTATTTCTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTAAAATTCAGGTAGCTCATCAAGAAAAAGCa                                                                                                                                                                                                                             <  2:472038/167‑1 (MQ=255)
                                                                                     aatggtcGGTGGGGTTGGGGTTCAGCCCCGCAACAAGCTGGAAACGGGTTGGATAGG‑TTATTTTTG‑CTCTGGTGCGTG‑AAAGGGGGTTCTGGCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACTCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCa                                                                                                                                                  <  2:1068095/233‑1 (MQ=255)
                                                                                     aaTGGCCGGTAGGGCTGGGATTCATCGCCGCAAAAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCa                                                                                                                                                  <  1:1092832/239‑1 (MQ=255)
                                                                                         tCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCCAGAAAAAGCACGCCGTTATGCGCCACGGAAATTT‑CACCGGGCCCTGGAATAGCGCCCCCCCCCACCACCCCAGTAAACAAGACACtgg                                                                                                                                              >  2:581899/1‑238 (MQ=255)
                                                                                         tCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCa                                                                                                                                                                   >  1:1370868/1‑218 (MQ=255)
                                                                                         tCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAAAGAGGcccgtt                                                                                                                                              >  2:209756/1‑234 (MQ=255)
                                                                                           cGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTCCCATCGCAGTTAACGATGCACTG‑gga                                                                                                                                           >  1:551527/1‑239 (MQ=255)
                                                                                                     gggATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAg                                                                                                                                 <  1:237130/238‑1 (MQ=255)
                                                                                                            aTCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CAcc                                                                                                                                                                                                <  1:130500/170‑1 (MQ=255)
                                                                                                            aTCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACt                                                                                                                                                >  1:1197924/1‑218 (MQ=255)
                                                                                                             tccgcgcaatcaggtggtacggggTTGGTTAGT‑TTTTTTTTG‑GCCGTGTGCGTA‑GAAGGTGTATGCGCCG‑GGATGCAATCGGGCCGCGC‑AAGGCCTCCAGGGTACTCCTTCAAAATGTAGGTGGCCCACCAAGAAAAATCACGCCTTTATGCGCCAGCGAAATTT‑CACCGGGCCTTGGAATTGCGCCACCGACTACCAGCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACggg                                                                                                                        <  2:990463/218‑1 (MQ=255)
                                                                                                                 cgcAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAACTTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGCATTGGCCCACCCCCTACCATGCCAGTTAACGATGCACTG‑GGATAGGGGGGAG‑GGAACgggtctg                                                                                                                    >  2:1162828/1‑235 (MQ=255)
                                                                                                                 cgcAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGACTTGCGCCACCGCCTACCATCCCCATTAAAGAGGCCCTG‑TGTT‑GGGGTGAGGGGACCggcgcc                                                                                                                     >  2:957849/1‑234 (MQ=255)
                                                                                                                 cgcAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGTT‑GAggctgcctcttataacca                                                                                                                     >  1:1153888/1‑222 (MQ=255)
                                                                                                                         cgtGGAAACGGGCGGGATAGT‑TTATTTTTG‑CCCGTTGGGGTG‑AAAGAGGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCAt                                                                                                            <  1:31346/237‑1 (MQ=255)
                                                                                                                          gtgaatacggtttggttgtg‑TTTTTTTTG‑TTTGTGGGCGGA‑AAAGGTGGATCTGCCC‑GTCTTCAACTGGCTTTCGA‑AAGGCACCCGGTGTACCCCGTTCAAATTCAGGTAGCTACTCAAGAAAACGCCCGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCGGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCAt                                                                                                            <  2:1230833/219‑1 (MQ=255)
                                                                                                                          cggggaagggggTGGATAGG‑TTTTTTTTG‑GCTGGGTGGGTGAAAAGTGGGACTTGCCC‑GGTTTCAATCGGCTCTCAC‑AAGGAATCCAGTGTACGCGGTTCAAATACAGGTAGCCCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCAt                                                                                                            <  2:915658/231‑1 (MQ=255)
                                                                                                                           tGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGA‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCAt                                                                                                            <  1:1295190/237‑1 (MQ=255)
                                                                                                                           gggAAACGGG‑TGGGTGGGTTTTTTTTTG‑TTCGTGGGGGGA‑AAAGGTGGACCGGCCC‑GGATCCACTCGGCCCTCGC‑AAGCAACCCAGTGTACCCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCAt                                                                                                            <  2:850985/236‑1 (MQ=255)
                                                                                                                                  gggCTGGATGTG‑TTTTTTTTG‑CGCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGTTTCAATCGGCTCGCGA‑AAGGCATCAAGGTTACGCCGTTCAAATCCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGt                                                                                                   <  2:393866/239‑1 (MQ=255)
                                                                                                                                   ggCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGaa                                                                                                                                                                                     >  1:164113/1‑158 (MQ=255)
                                                                                                                                     ttGGATAGG‑TTATTTTTG‑CTCGTTTGGTTG‑AAAGATGGATCTGCCC‑GGATTAAATCGGCTCTCGC‑AAGGCTTCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTAc                                                                                                 <  1:1305576/237‑1 (MQ=255)
                                                                                                                                               ttttttttGCCTCGTGTGCGTA‑AAATATGGACCTGCCC‑GGATTCATGCGGCTCTCGA‑AAGGCATCCAGGTTACGCCGTTCAAATTCAGGTAGCCCAGCAAGAAAAAGCCCGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATGGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGc                                                                                        <  2:528467/236‑1 (MQ=255)
                                                                                                                                                 ttttttttgccggtggggggaaaaGATGGGTTGGCCG‑GGTTTCAATCGGCTCTCGA‑AAGGAATCCGGTGTCCGGCGTTCAAATTCAGGGAGCCCATCAAAAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCCCCGCCTACCATCGTAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCa                                                                                       <  2:551527/218‑1 (MQ=255)
                                                                                                                                                     ttG‑CTCGGTTGCGTG‑AAAGATGGATCGGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTAcc                                                                                 <  1:957849/239‑1 (MQ=255)
                                                                                                                                                      tG‑CTCGGTTGCGTT‑AAATAGGGATTCGCCC‑GGATTCAAGCGGCTCTCGA‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCa                                                                                <  1:1232563/239‑1 (MQ=255)
                                                                                                                                                         cTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CCCCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCCCTG‑TGAT‑GAGGTGAGCCGAAGGGGCGCTGCCGCCCTTGTTTTTGTCTTGATTCAGCATTTACCAg                                                                               >  2:504347/1‑238 (MQ=255)
                                                                                                                                                         cTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GGGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGTTTCAGCATTTACCAg                                                                               >  2:1022648/1‑238 (MQ=255)
                                                                                                                                                          tctttggCGTG‑AAAGA‑GGCTTTGCCCGGGTTTAAACCGGTCCTCGC‑AAGGCATCAAGGTTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CCCCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGa                                                                              <  2:1032916/232‑1 (MQ=255)
                                                                                                                                                             tgtgCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAAAGGGCGCTGCCGCCATTGTTTTTGTACTGATTCCGAATTTACCCGACTTa                                                                          >  2:82469/1‑239 (MQ=255)
                                                                                                                                                               ggCGTG‑AAAGATGGACTGGCCG‑GGATTCAATCGGCCCTCGA‑AAGGCATCAAGTGTACGCGGTTCAAATCCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTaa                                                                         <  1:420453/237‑1 (MQ=255)
                                                                                                                                                                     taaaGGTGGATGCGCCA‑GGTTTAACGCGTCTCCCGA‑AAGGCACCCAGGTTACGCCTTTCAAATCCGGGTACCCAAACAAAAAAAAGCACGCCGTTAGGCGCCAGCGAAATTT‑CCCCGGGCCCTGGAATTGCGCCACCGCCTCCCAGCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCg                                                                    <  2:458738/237‑1 (MQ=255)
                                                                                                                                                                             gATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTAcc                                                                                 <  1:126685/217‑1 (MQ=255)
                                                                                                                                                                             gATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGACTTTAcc                                                                                 >  2:126685/1‑217 (MQ=255)
                                                                                                                                                                                cggCCG‑GGATTCAATCGGCTCCCGA‑AAGGCACCCAGTTGACGCCGTTCAAATTCAGGTAGCCCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTcc                                                         <  1:209756/236‑1 (MQ=255)
                                                                                                                                                                                 tccgc‑GGTTTCAATCGCCTCTCGA‑AAGGCATCCAGTGGACGCCGTTCAAATTCAGTTAGCTCATCAAGAAAAAGCACGCCGTTAGGCGCCAGCAAAATTT‑CACCGGGCCCTGGAATGGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAg                                                       <  2:613233/235‑1 (MQ=255)
                                                                                                                                                                                   ctcccgagttaaTCGGGTCTCGCGAAGGAATCAGGTTGACGCCGTTAAAATTCAGGTACCCCAGCAAGAAAAAGCACGCCGTTATGCGCCATCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAg                                                       <  2:514514/229‑1 (MQ=255)
                                                                                                                                                                                                       cGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCCGAACTCCCCAATCCCCC‑TTCATTGCTAAAAtt                                 >  2:1146639/1‑238 (MQ=255)
                                                                                                                                                                                                              gCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCCTTGTTTTTGTACTGATTCAGCATTTACCAGCCTTAATATCGCCACACTCTCCAGAGCCAC‑TTCATTGCTTAAATCAGGCaa                           >  2:954722/1‑239 (MQ=255)
                                                                                                                                                                                                               cttCCAGGGTCCGCCGTGCAAATTCAGGTAGCCCATCAAGAAAAAGCCCGCCTTTATGCCCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCaa                           <  1:655081/236‑1 (MQ=255)
                                                                                                                                                                                                                 tccgggttagccctgtaaaaTTCAGGTAGCATACTAAGGAAAAGCACGCCTTTTTGCGCCAG‑GAAATTTCCACGGGGCCTTGGATTTGCACCACCGCCTACCATCGCAGTTAACGATGC‑CTGTTGAT‑GAGGCGAGGTGAATGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAg                        <  2:1056099/222‑1 (MQ=255)
                                                                                                                                                                                                                 tCCAGTTGACGCCGTTAAAATTCAGGTGGCTCATCAAGAAAAAGCCGGCCGTTATGCGCCACCGAAATTT‑CCCCGGGCCCTGGAATTGCCCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACa                         <  1:851614/238‑1 (MQ=255)
                                                                                                                                                                                                                 tCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACg                                                                                                                                                       <  2:1197220/115‑1 (MQ=255)
                                                                                                                                                                                                                 tCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACg                                                                                                                                                       >  1:1197220/1‑115 (MQ=255)
                                                                                                                                                                                                                 tCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGCT‑GAGGTTAGCGGAACGGGCGCTGCCGCCATTGTTTTTTTTCTGGTTCGCCATTTACCGGACTTAATATCGCACCACTTTCCAGTGACCC‑TTCATTGCTAAAAACTAGCacaag                        >  2:617179/1‑235 (MQ=255)
                                                                                                                                                                                                                 tCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCAACCGCCTACCATCGCAGTTAACGATGCCCTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGCCTTAATATAGCAGCACTCTCCATTGCCTC‑TTCATTGCTTAAATTTGGCcaca                         >  1:1016668/1‑238 (MQ=255)
                                                                                                                                                                                                                 cccAGTTTCCCCCTTTAAAATTCAGGTAGCCAAAAAAAAAAAAGCGCGCCTTTATGCGCCAGGGAATTTT‑CACCGGGCCCTGGAATTGCGCCCCCGCCTCCCATCGCAGTTAACGATGCACTG‑TTAT‑GAGGTGAGCGGAACGGGGGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAg                        <  2:703200/238‑1 (MQ=255)
                                                                                                                                                                                                                  ccAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCCGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCACCAg                        >  1:1364089/1‑238 (MQ=255)
                                                                                                                                                                                                                       ttagcccGTACAAATTCAGGTAGCCCACCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGcc                     <  1:1061518/231‑1 (MQ=255)
                                                                                                                                                                                                                       gtACGCCGTTCAAATTCAGGTAGCCCATGAAGAAAAGCGACCCCGTTATGGGCCAGGGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGcc                     <  1:449385/236‑1 (MQ=255)
                                                                                                                                                                                                                        tACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATGGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCgcagca                                                               >  2:338270/1‑194 (MQ=255)
                                                                                                                                                                                                                           gCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAAt               >  1:969705/1‑238 (MQ=255)
                                                                                                                                                                                                                                tCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAATACg            >  1:331404/1‑236 (MQ=255)
                                                                                                                                                                                                                                tCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGCACTGCTTCAGCATTTAGCAGACTTAATATTGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAAGCCATTAAAAcagat         >  2:49407/1‑235 (MQ=255)
                                                                                                                                                                                                                                tCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGAACTGATTCAGAATTTACCAGACTTACAATCGCAGAACTCTCCAGTGCCTC‑TTCATTACTTAAATCTGGAAAAAGGCAATTAAAACGGcg         >  2:944222/1‑238 (MQ=255)
                                                                                                                                                                                                                                tCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACAATGCACTG‑TGAT‑GCGGGGCGCGCAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGCAGTAATATCGAAGCACTCTCCA‑TGCCCCATACCTTGCTAAAATTTGGAAACAGGACCTTAAAACgccg         >  2:42222/1‑236 (MQ=255)
                                                                                                                                                                                                                                       cAGGTAGCTCAACAAAAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAATACGGCTGGCGAGc  <  1:942383/239‑1 (MQ=255)
                                                                                                                                                                                                                                       cAGGTACCTCATCAAGAAAAAGCCCGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAATACGGCTGGCGAGc  <  1:1162828/239‑1 (MQ=255)
                                                                                                                                                                                                                                       cAGGTACCTCACCAAGAAAAAGCCCGGCTTTCTCCGCCAGGGAAATTG‑CACCGGGCTCTGGAATTGAGCCCCCGCGCACCATCGCAGTTACCGTGGCATTG‑TGAT‑GAGGTGGGCGGAACGGGCAGTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAATACGGCTGGCGAGc  <  2:1013050/239‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          
TCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGG‑TTATTTTTG‑CTCGTGTGCGTG‑AAAGATGGATCTGCCC‑GGATTCAATCGGCTCTCGC‑AAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTT‑CACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTTAACGATGCACTG‑TGAT‑GAGGTGAGCGGAACGGGCGCTGCCGCCATTGTTTTTGTACTGATTCAGCATTTACCAGACTTAATATCGCAGCACTCTCCAGTGCCTC‑TTCATTGCTTAAATCTGGCAACAGGCCATTAATACGGCTGGCGAGC  >  NC_000913/3948830‑3949294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A