Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F20 I40 R1	12	45.4	1473824	96.3%	1419292	148.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,029,079	C→T	A188T (GCT→ACT)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,029,079	0	C	T	95.8%	126.7 / NA	48	A188T (GCT→ACT)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (17/29); minor base G (2/0); total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTT  >  CP009273/3028934‑3029222

gggTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTa                                                                                                                                              >  1:85150/1‑149 (MQ=255)

gggTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTATACGCAGCCCGCCGTAAGTGTa                                                                                                                                              >  1:84968/1‑149 (MQ=255)

  gTTGATTTCGATATCAATATCATCATCAACTGCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGCCAAACGGGCTTTTACGCACCAGCCGGTGAACGCCGGTTTCGTTACGCTGACAGCCCTAAGGGTact                                                                                                                                            >  2:642423/1‑147 (MQ=255)

       tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCg                                                                                                                                       <  1:267395/149‑1 (MQ=255)

       tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGGGTAATCGCCg                                                                                                                                       >  1:304867/1‑149 (MQ=255)

               tcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCCCCGGCGAtttt                                                                                                                               >  1:686556/1‑149 (MQ=255)

                caatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGACGACGACCGCCGGGGTCAAACGGGCTTTTACGCACCAGCCGGGGAACGCCGGTTGCTGTACGCAGCCGGCCGTAAGTGTAATCTCCGgagagtgtg                                                                                                                              >  1:317564/1‑144 (MQ=255)

                  atatcaTCATCAACTTCCGGATAAACAACCGCGGAGCTGAACGACGTGGGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGAt                                                                                                                            <  1:11103/149‑1 (MQ=255)

                          atccACTTCCGGATAAACAAACGGGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCGGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACgg                                                                                                                    <  2:273794/149‑1 (MQ=255)

                                ttCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGCTTTTGATCGTCACGGCTTTaa                                                                                                              >  1:273650/1‑149 (MQ=255)

                                ttCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTaa                                                                                                              >  1:273739/1‑149 (MQ=255)

                                     gATAAACAAACGCGGAGCTGAACGACGTGTGGTGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATAccc                                                                                                         <  1:501359/149‑1 (MQ=255)

                                             agcgcgGAGCGGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCTGGACGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTc                                                                                                 <  1:506958/147‑1 (MQ=255)

                                             aacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTc                                                                                                 <  1:608084/149‑1 (MQ=255)

                                                    cgCTTAACGACGTGTGGCGCCGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGCTGAACGCCAGTTTCTGTCCGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTcc                                                                                          <  2:302416/148‑1 (MQ=255)

                                                         aaCGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGActc                                                                                     >  1:125016/1‑149 (MQ=255)

                                                                 gtgGCGACGACCGCCGGAGTCAAACGGGCTTTTCTGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCgatga                                                                             <  2:249477/149‑1 (MQ=255)

                                                                 gcggCGACGACCGCCGGCGTCAAACGGGCTTTTACTCCCCCGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCgatga                                                                             <  2:588157/147‑1 (MQ=255)

                                                                     cgacgaCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGAAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCCCCTTCCGACTCTGCGATGATTTc                                                                         >  1:105872/1‑149 (MQ=255)

                                                                      ggcgcgggccgcggTCAAACGGGGTGTTACGCACCAGGCGGTGAACGCCGGTTGCGGGCCGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTTATCGTCACGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                        <  2:135976/136‑1 (MQ=255)

                                                                         tacCGCCGGAGTCAACCGGGATTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGAAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATGCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtt                                                                     <  1:465757/148‑1 (MQ=255)

                                                                          acCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCGGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTGCATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGttt                                                                    <  2:567728/149‑1 (MQ=255)

                                                                           ccgccgGGGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCGGGTTTCTGTACGCAGCCAGCCGTAAGTGTAAGCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                   <  2:23959/149‑1 (MQ=255)

                                                                           ccgccgGAGTCAAACGGGCTTTTACGCACCAGGCGGGGAACGCTGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                   <  2:723657/149‑1 (MQ=255)

                                                                           ccgccgGAGTCAAACCGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                   <  1:56804/149‑1 (MQ=255)

                                                                                ggAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGACGTAAGTGGAATCGCCGGAGATTTTGATAGTCCCGGATTTCGAAACCGCCACTGCACCTTCCGACTCTTCGGTGATTTCAGTTTTGAAAc                                                              >  2:166951/1‑149 (MQ=255)

                                                                                   gTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAc                                                           <  1:486877/149‑1 (MQ=255)

                                                                                    ccAAACGGGCTTTTACGCACCAGGCGGTGACCGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGACCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcg                                                          <  2:108518/148‑1 (MQ=255)

                                                                                      aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCCGTTTTGGAACCAcgcg                                                        >  2:444217/1‑149 (MQ=255)

                                                                                                  aCGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgc                                            <  1:138505/149‑1 (MQ=255)

                                                                                                   cgtaCCGGGCGGTGAACGCCGGTTCCTGTACTCTGCAAGCCGTAAGTGGAATCGCCGGAGATTTTGATCTTCATGAATTCAATACCCGCCACTTCACATTTCGACTCTTCGATGATTTCGGTTTTGAAACCACGCGATTCTGCCCAgcg                                           <  2:85150/146‑1 (MQ=255)

                                                                                                   cGCACCAGGCGTGGAATGCCGGTTTCTGTACGCAGCCAGCCTGAACTGTAATCGCGGGAGATTTTGTTCGTCCCTGTTTTAATACCCTCCACTTCGCCGTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcg                                           <  2:84968/149‑1 (MQ=255)

                                                                                                   cGCACCACGCGGTGACAGCCGGCTTCTGTACGCCACCAGCCGTAAGTGTAATATCCGGAGATTTTAATCGTCACGGATTTAGTAGCCGCCACTTCCCCTCCCGAGTCATCGCTGATTTCAGTTTTGAAACCACCCGATTCTGCCCAgcg                                           >  2:283252/1‑149 (MQ=255)

                                                                                                        cAGGCGTCGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCCCCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCTCAGCGCAGat                                      <  2:637659/149‑1 (MQ=255)

                                                                                                          ggCGGTGAACGCCGGTTCCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatac                                    <  1:459715/149‑1 (MQ=255)

                                                                                                          ggCGGGGAACGCCCGTTTCTGTACGAAGCCAGCCGTACGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGTCTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatac                                    <  1:405782/149‑1 (MQ=255)

                                                                                                             ggTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata                                 <  1:667919/149‑1 (MQ=255)

                                                                                                                    gCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                          >  1:72398/1‑149 (MQ=255)

                                                                                                                    gCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATAAATACGCTCaa                          >  2:400538/1‑149 (MQ=255)

                                                                                                                    gCCGGTTTCGGTACGCTCCCAGCCGGAAGTGTAATCGCCGGTGATTTTGATCGTCACGGATGTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                          <  1:4251/149‑1 (MQ=255)

                                                                                                                          ttCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGc                    <  1:289078/149‑1 (MQ=255)

                                                                                                                            cTGTCCGCACCCAGCCGTAAGTGCAATCGCCGGAGATTTTGACCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTc                  <  2:731263/149‑1 (MQ=255)

                                                                                                                              gTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATCTAATACCCGCCACTTCACCTTCCCACTCTTCGATGATTTCAGTTTTGATACCACGCGATTCTGCCCAGCGCATATACATAAGCTCAAACATGCTccc                >  2:286859/1‑147 (MQ=255)

                                                                                                                                 cGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTGAAAGCAGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCa             >  1:721414/1‑149 (MQ=255)

                                                                                                                                      ccagccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCt        >  1:159142/1‑149 (MQ=255)

                                                                                                                                        agccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCtgt      >  2:99621/1‑149 (MQ=255)

                                                                                                                                           cGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCt   <  2:149210/149‑1 (MQ=255)

                                                                                                                                            gTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCtt  <  1:67277/149‑1 (MQ=255)

GGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTT  >  CP009273/3028934‑3029222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A