breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 299,113 IS5 (–) +4 bp coding (412‑415/615 nt) yagU → DUF1440 family inner membrane protein
RA 753,013 G→T E551* (GAA→TAA)  sdhA → succinate dehydrogenase, flavoprotein subunit
RA 1,737,874 G→A W54* (TGG→TAG)  mdtK → multidrug efflux system transporter
RA 2,718,678 C→G G143A (GGC→GCC)  kgtP ← alpha‑ketoglutarate transporter
RA 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,861,917 G→A Q66* (CAG→TAG)  nlpD ← activator of AmiC murein hydrolase activity, lipoprotein
RA 2,870,339 G→A G134S (GGC→AGC)  iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 3,019,631 G→T M169I (ATG→ATT guaD → guanine deaminase
RA 3,029,079 C→T A188T (GCT→ACT)  prfB ← peptide chain release factor RF‑2
RA 3,960,789 C→G T338R (ACA→AGA)  rho → transcription termination factor
RA 4,049,411 G→T Q97K (CAG→AAG)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,390,205 G→T G34V (GGG→GTG)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 345714 346928 1215 18 [16] [16] 17 prpC–[prpD] prpC, [prpD]
* * ÷ CP009273 748619 749962 1344 17 [15] [16] 17 gltA gltA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 287112NA (NA)46 (1.020) 34/296 0.4 100% noncoding (1/768 nt) IS1 repeat region
?CP009273 = 2269346 0 (0.000)coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
* ? CP009273 360104 =NA (NA)62 (1.380) 48/296 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 104600148 (1.070)29 (0.640) 23/296 1.2 53.2% intergenic (+15/+418) insB1/cspH IS1 transposase B/stress protein, member of the CspA‑family
?CP009273 2269338 = 3 (0.070)coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
* ? CP009273 1203246 =15 (0.330)36 (0.900) 28/264 0.5 69.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 18 (0.450)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326114 (0.310)34 (0.850) 30/264 0.4 69.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 18 (0.450)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1391495NA (NA)35 (0.780) 29/296 0.7 100% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 0 (0.000)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =0 (0.000)52 (1.160) 33/294 0.4 100% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region