Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F184 I0 R1 46 69.8 1527574 94.8% 1448140 140.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 874,718 A→C 100% K541N (AAA→AAC)  clfA → fibrinogen‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730874,7180AC100.0% 242.5 / NA 77K541N (AAA→AAC) clfAfibrinogen‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (55/22);  total (55/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTA  >  CP000730/874582‑874851
                                                                                                                                        |                                                                                                                                      
aTCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCa                                                                                                                                     <  2:556177/140‑1 (MQ=255)
  ccGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTACCGATAACCAAgt                                                                                                                                   >  2:281307/1‑140 (MQ=255)
     aaTAGCAAATGTGATTTAGCTTTACGTTCAACTATATATGGGTATAGCCCAAAAACAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAgttgt                                                                                                                                <  1:358157/140‑1 (MQ=255)
        aGCAAAGGTGATTTCGCTTTACGTTCAACTTTATATGGGTCTCACTCGTATATAATTTGGCGCTATATGTCATGGGACAACGAAGTAGCATGTAATCACGGATCAGGTTCTGGTGACGGTATCGATCACCCAgttgttgc                                                                                                                             >  1:3260/1‑138 (MQ=255)
        aGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGTTAACCCAGTTGTTcc                                                                                                                             >  2:27441/1‑140 (MQ=255)
        aGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATGACCCAGTTGTTcc                                                                                                                             >  2:27326/1‑140 (MQ=255)
        aGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTcc                                                                                                                             >  2:643064/1‑140 (MQ=255)
         gCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCt                                                                                                                            >  2:152569/1‑140 (MQ=255)
           aaaGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGa                                                                                                                          >  1:631907/1‑140 (MQ=255)
            aaGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCAAAAACCAAGTTGTTCCTaaa                                                                                                                         >  1:319519/1‑140 (MQ=255)
            aaGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGATGACGGTATCGATAACCCAGTTGTTCCTGaa                                                                                                                         >  1:225036/1‑140 (MQ=255)
                        cTTTACGTTCAACTTTATATGGGTATAACTCGTATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAg                                                                                                             <  2:424233/140‑1 (MQ=255)
                        cTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAg                                                                                                             <  2:586920/140‑1 (MQ=255)
                          ttACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGc‑c                                                                                                          >  2:354967/1‑140 (MQ=255)
                                tCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGa                                                                                                    >  2:108915/1‑140 (MQ=255)
                                 cagcTTTAAATTGGTATAATGCGATTATTATTTGACGCTCTATGTCAGGGGTCAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTGGTTCCTGACAAACCTGATGAGC‑CTGGTGaa                                                                                                   >  2:256764/4‑140 (MQ=255)
                                 cAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGaa                                                                                                   >  1:272626/1‑140 (MQ=255)
                                 cAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGaa                                                                                                   >  2:700301/1‑140 (MQ=255)
                                  aaCTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTGTCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGaaa                                                                                                  >  2:226490/1‑140 (MQ=255)
                                      ttaagtGGCTATAACCCGAATATAAGTTGGCGCGCTATGGCCTGGGGCAACGAAGTAGCATTAAATAACGGATCAGGTACTCCTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGa                                                                                              <  2:199710/135‑1 (MQ=255)
                                      ttATATGGGTATAACTGGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGa                                                                                              <  2:233741/140‑1 (MQ=255)
                                       tataTGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGaa                                                                                             >  2:472561/1‑140 (MQ=255)
                                        atatGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATGGAAc                                                                                            >  1:198979/1‑140 (MQ=255)
                                        atatGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGAATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAAc                                                                                            >  1:636603/1‑140 (MQ=255)
                                        atatGGGTATAACTCGAATATAATTTGGCGCTCTATGTAATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGCTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAAc                                                                                            >  1:422849/1‑140 (MQ=255)
                                        atatGGGTATAACTCGAATATAATTTGGCGCTCTATGTAAAAGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAAc                                                                                            >  1:333951/1‑140 (MQ=255)
                                         tatGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAAcc                                                                                           <  1:500434/140‑1 (MQ=255)
                                          atGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAAcaa                                                                                          >  1:272113/1‑138 (MQ=255)
                                                 taACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCag                                                                                   >  2:412946/1‑140 (MQ=255)
                                                   aCTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCagag                                                                                 >  2:313425/1‑140 (MQ=255)
                                                   aCTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCagag                                                                                 >  1:718696/1‑140 (MQ=255)
                                                     tCGAATATAATTTGGCGCTCTATGTCATTAGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGa                                                                               <  1:746059/140‑1 (MQ=255)
                                                     tCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGa                                                                               >  1:573816/1‑140 (MQ=255)
                                                     tCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTAGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACTAATTCCAGGGGa                                                                               >  1:68981/1‑140 (MQ=255)
                                                     tCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGAACAGGTTCTGGTGACGGTATCGATAACCCAGTTGGTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCCGAGGa                                                                               >  2:663693/1‑140 (MQ=255)
                                                     tCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGAAGCATTTAATAACGGATCAGGTTCTGGTGACGGAATCGATAACCCAGTTGTTACTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATAGCAGAGGa                                                                               >  2:380332/1‑140 (MQ=255)
                                                     tCGAATATAATTTGGCGCTCTATGCCATCCAGCGACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGATC‑CTGGTGAAATTGAACCAATTCCAGAGGa                                                                               >  1:367207/1‑140 (MQ=255)
                                                      cGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAACTTGAACCAATTCCAGAggct                                                                              >  2:118265/1‑138 (MQ=255)
                                                      cGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGA‑CTGGTGAAATTGAACCAATTCCAGAGGAt                                                                              >  1:721500/1‑140 (MQ=255)
                                                       gAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATTACGAATCAGGTTCTGGTGACGGTATCGATAACCCAGTTATTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTGCACAGGAtt                                                                             >  1:294636/1‑140 (MQ=255)
                                                          tataATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGCATTCAg                                                                          >  2:184129/1‑140 (MQ=255)
                                                           ataATTTGGCGCTCTATGTCATGGGATAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGa                                                                         <  1:123085/140‑1 (MQ=255)
                                                           ataATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGa                                                                         >  2:452702/1‑140 (MQ=255)
                                                                     gcTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGACCCAATTCCAGAGGATTCAGATTCTGGcccg                                                               >  2:716469/1‑139 (MQ=255)
                                                                         tATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGtt                                                           >  2:590171/1‑140 (MQ=255)
                                                                                   gACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACTCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTgg                                                 <  1:37360/140‑1 (MQ=255)
                                                                                   gACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTgg                                                 >  2:477994/1‑140 (MQ=255)
                                                                                   gACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATACTGACCCAGGTTCAGATTCTgg                                                 >  1:667445/1‑140 (MQ=255)
                                                                                   gACAACAAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTgg                                                 >  2:477915/1‑140 (MQ=255)
                                                                                    aCAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGc                                                >  2:282495/1‑140 (MQ=255)
                                                                                     cAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATCCAGATTCTGACCCAGGTTCAGATTCTGGCa                                               >  2:114808/1‑140 (MQ=255)
                                                                                      aaCGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCGAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAg                                              >  1:98942/1‑140 (MQ=255)
                                                                                       aCGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGTCAACCGCTAG‑CAACTTGAACCAATACCAAAGGATTAAGATACTGACCCAGAATAAAATTCTGGAAGc                                             >  1:361712/1‑140 (MQ=255)
                                                                                          aaGTAGCATTTAATAACGGATCAGGTTCTGGTGATGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAAATGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGAt                                          <  1:72819/140‑1 (MQ=255)
                                                                                          aaGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGAt                                          >  2:752112/1‑140 (MQ=255)
                                                                                           aGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGCTTCAGATTCTGACCCAGGTTCAGATCCTGGCAGCGGtt                                         >  1:224496/1‑140 (MQ=255)
                                                                                               gCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCAAGAGGAATCAGATTCTGACCGAGGTTCAGATTCTGGCAACGATTCTaa                                     >  1:108058/1‑140 (MQ=255)
                                                                                                     aataaCGGTTCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGa                               <  1:119826/140‑1 (MQ=255)
                                                                                                     aataaCGGATCAGGTTCTGGTGACGTTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGa                               <  1:335156/140‑1 (MQ=255)
                                                                                                     aataaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGa                               >  1:430382/1‑140 (MQ=255)
                                                                                                     aataaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGa                               <  2:31303/140‑1 (MQ=255)
                                                                                                        aaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCATATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAg                            >  2:730018/1‑140 (MQ=255)
                                                                                                        aaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACGCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAg                            <  1:354967/140‑1 (MQ=255)
                                                                                                          cGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCACTTCCAGAGGATTCAGATTCTGCCACAGGTCCAGCTCCTGGCAGCGATTCTAATTCAGACAGct                          <  2:636603/140‑2 (MQ=255)
                                                                                                           ggATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGGTTGCgg                         <  2:658825/140‑1 (MQ=255)
                                                                                                             aTCAGGTTGTGGTGACGGTATCGATAACCCGGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCGGATAGCGGtt                       <  2:514339/140‑1 (MQ=255)
                                                                                                             aTCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGtt                       <  1:90914/140‑1 (MQ=255)
                                                                                                               cAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCAAGGTTCAGATTCTGGAAGCGATTCTAATTCAGATGGCGGTTCa                     >  1:603125/1‑140 (MQ=255)
                                                                                                                 ggTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATCGAGATGCTGACCCAGGTTGAGATTCTGGCAGCGATCCTAATCCATAGAGGGGCTCAGa                   >  2:248689/1‑140 (MQ=255)
                                                                                                                  gTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTCACCCAGGTTCAGATTCTGGCCGCGATTCTAATTTAGATAGCGTTTTAGAt                  >  1:730529/1‑140 (MQ=255)
                                                                                                                     cTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCg               >  2:334793/1‑140 (MQ=255)
                                                                                                                     cTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCg               >  1:373178/1‑140 (MQ=255)
                                                                                                                         tGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCgggga           >  2:18617/1‑138 (MQ=255)
                                                                                                                                aTCGATAACCCAGTTGTTACTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGAGAGCGGTTCAGATTCGGGTAGTGATTc    <  1:663693/140‑1 (MQ=255)
                                                                                                                                 tCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCt   <  2:647336/140‑1 (MQ=255)
                                                                                                                                 tCGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATCCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCt   <  1:716469/140‑1 (MQ=255)
                                                                                                                                  cGATAACCCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATACCAGAGGATTCAGATTCTGACCCAGGTACAGAATCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTa  >  1:606782/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGC‑CTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTA  >  CP000730/874582‑874851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A