Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F109 I0 R1
|
32 |
27.2 |
584098 |
96.5% |
563654 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
1,232,465 |
G→C |
100% |
A124P (GCG→CCG) |
USA300HOU_1157 → |
non‑specific serine/threonine protein kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 1,232,465 | 0 | G | C | 98.0%
| 49.2
/ ‑6.4
| 19 | A124P (GCG→CCG) | USA300HOU_1157 | non‑specific serine/threonine protein kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (8/10); minor base A (0/1); total (8/11) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCA > CP000730/1232329‑1232598
|
agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATccgc < 2:22643/140‑1 (MQ=255)
agaTGACTGGTACTACTTAGTAATGGAATATATCGAAGGTCCGTCTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATg < 2:136737/140‑1 (MQ=255)
agcaCTGTTACTACTTAGTAATGGAAAAAATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTGAACATCCGCATGat < 1:152322/137‑1 (MQ=255)
tactacTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGcatgt > 1:132904/1‑136 (MQ=255)
actTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTa > 2:225027/1‑140 (MQ=255)
ataaCGAAGGTCCGAATTGGGCTGAGGATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATGAAg < 2:10038/140‑1 (MQ=255)
tgccGATTTTGTCGGAGGATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGTCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAat < 2:291080/138‑1 (MQ=255)
tatTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACACCCGTATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATa < 2:37756/140‑1 (MQ=255)
aaGTCATGGGACAATAAGTGTTAACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGt < 1:32967/140‑1 (MQ=255)
aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGTTATTTAGCCACTAAATATATTAATTTAAAGCAATAAAACGTTGaaa > 1:118287/1‑140 (MQ=255)
gCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAATACATGTATTACATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAatat < 1:239170/140‑1 (MQ=255)
aGTGTTGAAACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTCAAAATATTTGAttt < 1:151748/140‑1 (MQ=255)
tGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGaa < 1:27979/140‑1 (MQ=255)
aGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTa > 1:250947/1‑140 (MQ=255)
aaacAAATATTGGAAGGCATAAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGAc < 1:282099/137‑1 (MQ=255)
atatTGGATGGCATTAAACATACGAATGATATGCGTATTGTAAATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCttt < 1:121707/140‑1 (MQ=255)
ttGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAAc > 2:129234/1‑140 (MQ=255)
tGGAAGGCAAAAAACATCCGAATGATATGCGTATTGTACATAGAGATGTTAACCCACAAAATCTTGTAATTAACAGCAATAAAACGTTGAAAATATTTGATCTTGGAATTGCTTAAGCGATAAGTGAGACGTCTTTaatt > 2:186705/1‑138 (MQ=255)
aTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATCTGATTATGGAATTGCGAAAGCTATAAGTGAGACGTCTTTAACTCAg > 1:122057/1‑140 (MQ=255)
aaaCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCa > 1:122491/1‑140 (MQ=255)
|
AGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCA > CP000730/1232329‑1232598
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
