Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F116 I0 R1
|
44 |
26.4 |
562224 |
96.5% |
542546 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
2,664,466 |
G→A |
52.7% |
A65T (GCG→ACG) |
USA300HOU_2518 → |
acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,664,466 | 0 | G | A | 52.7%
| ‑1.0
/ 23.5
| 20 | A65T (GCG→ACG) | USA300HOU_2518 | acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base A (5/5); total (9/11) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-02 |
GCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT > CP000730/2664331‑2664606
|
gCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTacgacg < 1:275649/141‑1 (MQ=255)
aTTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTc > 2:38060/1‑141 (MQ=255)
aTCAATACGACAGCTATTTATACTTATGAACCGCAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCGTAGGGTTTGCGACGTTCGGt < 1:175620/141‑1 (MQ=255)
aGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGAcc < 2:134988/141‑1 (MQ=255)
gCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCt < 1:168074/141‑1 (MQ=255)
cTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCtt > 1:46618/1‑141 (MQ=255)
aaCCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCtat < 1:201412/141‑1 (MQ=255)
acaAACCATAGACGAACGTGTAGCATAGTTTGAAACGAAACAACGTAAGAATGAGCCTATTTTTGTATTTGAGGAAAATGGAAGTATCTTAGGGTTTACGAGGTTCGGTTCATTTAGACCTTGGCCAGCATAcatgtagac > 2:228805/1‑133 (MQ=255)
tAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCGTTTATGCCTTGGCCGGCATCCCTATATACAATCGaaa > 1:6666/1‑140 (MQ=255)
aGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTAGTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTCTATACAATCGAACa > 2:32656/1‑141 (MQ=255)
aCGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTAt < 1:18284/141‑1 (MQ=255)
gttGAAAGCAAACAACTGAAGCATGAGCCTATCCTTGTATGTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTGCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCt < 2:240905/140‑1 (MQ=255)
aaacAACGTAAGCATGAGCCTTTCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTaga < 2:219531/141‑1 (MQ=255)
aacGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGaa > 1:87347/1‑141 (MQ=255)
gCATGGGCCTATCTTTGGATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCCTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTAt < 2:144765/141‑1 (MQ=255)
aTGAGCCTATCTTTTTATTTGAGGAAAAGGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTACATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTg < 2:81205/141‑1 (MQ=255)
cTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTc < 1:38060/141‑1 (MQ=255)
gAAAATGGAAATGCCTTAGAGTTTACGACTTTCGGTTCATTTAGACCTTTTCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTa > 2:205816/1‑141 (MQ=255)
aaTGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAAtt > 1:123273/1‑141 (MQ=255)
aGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGaa > 1:236211/1‑141 (MQ=255)
cTTAGGGTTTTGGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCaaa < 2:262332/141‑1 (MQ=255)
gCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGt < 1:210681/141‑1 (MQ=255)
|
GCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT > CP000730/2664331‑2664606
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
