breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 39,378 C→T 100% D586N (GAT→AAT)  mecA ← penicillin binding protein 2 prime
RA 110,660 T→A 9.7% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 26.7% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 25.6% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 367,644 G→T 100% intergenic (‑96/‑20) nanA ← / → USA300HOU_0336 N‑acetylneuraminate lyase/possible transcriptional regulator
RA 406,961 C→G 61.3% A353G (GCA→GGA)  USA300HOU_0375 → acetyl‑CoA C‑acetyltransferase
RA 479,553 G→A 100% A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 512,643 A→G 41.8% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 535,044 A→G 15.3% intergenic (+4615/‑1110) USA300HOU_0494 → / → rplY hypothetical protein/ribosomal protein L25
RA 638,614 G→T 100% T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 646,838 G→A 55.0% intergenic (+192/‑395) lplA2 → / → mvaK1 lipoate‑‑protein ligase A/mevalonate kinase
RA 654,252 C→T 58.0% S362L (TCG→TTG)  USA300HOU_0591 → hypothetical protein
RA 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 790,778 G→A 100% intergenic (+277/‑197) USA300HOU_0741 → / → USA300HOU_0742 possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 942,060 C→G 46.4% intergenic (‑31/+218) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
RA 1,064,126 G→C 44.7% Y121* (TAC→TAG USA300HOU_1000 ← LytR family transcriptional regulator
RA 1,087,670 A→T 79.2% L5I (TTA→ATA)  USA300HOU_1021 ← hypothetical membrane protein
RA 1,232,465 G→C 100% A124P (GCG→CCG)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,274,212 A→T 58.8% K205N (AAA→AAT USA300HOU_1186 → CodY family transcriptional regulator
RA 1,296,417 C→T 13.4% A137V (GCG→GTG)  pnp → polyribonucleotide nucleotidyltransferase
RA 1,318,237 T→A 43.6% F124Y (TTT→TAT)  miaB → 2‑methylthioadenine synthetase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,420,434 G→A 100% A229T (GCG→ACG)  USA300HOU_1318 → M3 family oligoendopeptidase F
RA 1,440,007 Δ1 bp 100% coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G 100% A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,530,723 G→A 58.5% T188I (ACA→ATA)  USA300HOU_1396 ← glycosyltransferase
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,744,454 C→T 100% G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,775,556 G→A 47.2% P323L (CCT→CTT)  clpX ← S14 family endopeptidase ClpX
RA 1,797,772 G→C 100% R7G (CGC→GGC)  USA300HOU_1679 ← sensor histidine kinase
RA 1,834,119 G→T 67.9% intergenic (+68/‑46) USA300HOU_1710 → / → USA300HOU_1711 hypothetical protein/class V aminotransferase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,027,561 G→C 49.8% T129S (ACC→AGC)  vraS ← sensor histidine kinase VraS
RA 2,035,886 T→C 66.2% intergenic (‑212/‑3) USA300HOU_1892 ← / → USA300HOU_1893 hypothetical protein/DNA‑directed DNA polymerase III
RA 2,143,110 G→A 49.8% V163V (GTG→GTA USA300HOU_2026 → hypothetical membrane protein
RA 2,274,965 G→C 45.0% E106D (GAG→GAC bglG → transcriptional antiterminator
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,664,466 G→A 52.7% A65T (GCG→ACG)  USA300HOU_2518 → acetyltransferase
RA 2,679,708 G→A 64.6% W64* (TGG→TGA USA300HOU_2536 → possible secretory antigen
RA 2,719,269:1 (A)5→6 100% coding (213/705 nt) USA300HOU_2573 → dehydrogenase
RA 2,856,087 T→A 100% P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =25 (1.030)16 (0.950)
+40 bp
15/180 NT 45.7% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = 30 (1.230)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA