Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F59 I0 R1
|
88 |
34.1 |
751148 |
93.4% |
701572 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
1,105,994 |
T→C |
100% |
T12T (ACT→ACC) |
pdhD → |
dihydrolipoyl dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 1,105,994 | 0 | T | C | 100.0%
| 71.3
/ NA
| 24 | T12T (ACT→ACC) | pdhD | dihydrolipoyl dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/9); total (15/9) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGC > CP000730/1105863‑1106128
|
cacaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATc > 1:216345/1‑141 (MQ=255)
ttGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTc > 1:172842/1‑141 (MQ=255)
tGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCtgg > 1:255453/1‑141 (MQ=255)
atctGCAACTGGCCAACATTCCATGAATCACATTAAACGTTTATTCACTAAACCAGAACTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGTAGCAGGTCCtgg < 2:64493/138‑1 (MQ=255)
gCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATCCGTTg > 1:137553/1‑141 (MQ=255)
aaTGAATCACATTAAACGTTTATTAAATCATTCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCg > 1:370859/1‑141 (MQ=255)
aTGAATCGCATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATGCGTTGCAGCAATTCgg > 2:16292/1‑140 (MQ=255)
caTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGTAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCa > 1:78515/1‑141 (MQ=255)
aatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAGTTCAGGCTAAGAGTAACa < 2:172842/141‑1 (MQ=255)
ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAACAGGTCCTGGTGGATACGTTGCAGAAATTCATGCAGCACAATTAGGAAAAAAAGTAaaaaag > 2:168841/1‑139 (MQ=255)
cAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAACAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCg < 1:325795/141‑1 (MQ=255)
attattatgAATGGAAGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGCCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTga < 1:16292/141‑1 (MQ=255)
attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATGCGTGCAGCTCAATTAGGACAAAGAGTAACAATCGTTGAGaa > 1:34577/1‑141 (MQ=255)
attgggtGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg < 1:214045/135‑1 (MQ=255)
ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtgg > 1:338989/1‑141 (MQ=255)
aaCATGGTAGTTAGAGATTTCCCAATGGAAACAGATACCAGAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAAGTCGTGGAGCTCAATTAAGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGtt < 2:143326/141‑1 (MQ=255)
ctGGTAGTGGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGTTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTGTGc < 1:268038/140‑1 (MQ=255)
gTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTa > 1:314504/1‑141 (MQ=255)
aggtGGAGATTTCTCAATTGAAACAGATTGCATAGTGATCGTAGGAGGTTTTGGTATATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTaaa < 2:339421/138‑1 (MQ=255)
ggAGATTTCCCAATTGAAACCGATCCCATAATAATCGGAACAGGTCCTGGTGGATACGTTGCAGCAATTCGAACAGCTCATTTCTGACAAAAAGTAACAATCGATGAGAAAGGTAATCTTGCAGCTGTTTGCTTAAACGTa > 2:64391/1‑141 (MQ=255)
ttGAAACAGATACCATAGTAATCGGACCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTCAAACGTAGGATGTATTCCtt < 1:226205/141‑1 (MQ=255)
aCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaaa > 2:82416/1‑141 (MQ=255)
cAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAg > 2:188047/1‑141 (MQ=255)
aGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGc > 1:135660/1‑141 (MQ=255)
|
CACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGC > CP000730/1105863‑1106128
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
