Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F59 I0 R1 88 34.1 751148 93.4% 701572 140.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,337,959 C→T 100% intergenic (‑125/‑119) USA300HOU_2195 ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,337,9590CT100.0% 116.8 / NA 36intergenic (‑125/‑119)USA300HOU_2195/USA300HOU_2196possible MerR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/9);  total (27/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTGCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTACATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTGG  >  CP000730/2337833‑2338092
                                                                                                                              |                                                                                                                                     
aTTGCGACTATCCTTTCAGGTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGttttt                                                                                                                         >  1:243059/1‑141 (MQ=255)
    cGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAAtt                                                                                                                     <  1:344125/141‑1 (MQ=255)
      aCTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTAc                                                                                                                   <  1:167193/141‑1 (MQ=255)
       cTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACt                                                                                                                  >  1:244783/1‑141 (MQ=255)
          tCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTCTATTTCATCGTTTTTAATTACTTTa                                                                                                               >  1:131678/1‑141 (MQ=255)
          tCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTCATTACTTTa                                                                                                               >  1:131675/1‑141 (MQ=255)
          tCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTa                                                                                                               >  1:364167/1‑141 (MQ=255)
             ttttatttATGTTTTGTCGTCTAAAGTTATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTaaaa                                                                                                            <  2:140919/135‑1 (MQ=255)
             tttCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTAGTTTGTATACTTCTTATTTTATTACTCAAATTTAAATTTGTATCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTaaaa                                                                                                            >  2:47456/1‑141 (MQ=255)
                      tGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTTACATTTTTTCTTTCATCGTTTTTAATTATTTTAAAAATTGTATaa                                                                                                   >  2:49130/1‑141 (MQ=255)
                      tGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATaa                                                                                                   >  1:79773/1‑141 (MQ=255)
                              cgtcTAAAGTAATGTTGCTTTATATATTGTCATGTTCGTTTGAATGCTTCTTTTGTTATTGCTAAAATTTAAATTTGTTTCTTTTTTAACATTTTATATTTCATTGTTTTTAATTACTTTAAACATTGTTTAAATTaattt                                                                                           >  2:79004/1‑138 (MQ=255)
                              cgtcTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTTTTTTTTTTTATTTCATAGTTTTTATTTACTTTAAAAATTGTATAACTTAAatt                                                                                           >  2:218942/1‑140 (MQ=255)
                                 cTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAACATTGTATAACTTAAATAttt                                                                                        >  2:150246/1‑141 (MQ=255)
                                      gTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTaaaaa                                                                                   >  1:34167/1‑140 (MQ=255)
                                         aTGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTTTTTTTTTATATTTCATCGTTTTTAATTACTTTAAAAATTTTATAACTTAAATTTTTAAAATgtt                                                                                >  2:118681/1‑139 (MQ=255)
                                                    tatatTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTaa                                                                     <  1:172420/141‑1 (MQ=255)
                                                     atatTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAAtaatttca                                                                    >  2:277588/1‑134 (MQ=255)
                                                                 tCGTTTGAATTCTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGACAATAtt                                                        <  2:205291/141‑1 (MQ=255)
                                                                  cGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATAttt                                                       >  1:304357/1‑141 (MQ=255)
                                                                   gTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTa                                                      >  2:245135/1‑141 (MQ=255)
                                                                          tACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCAATAAGATTGATAATATTTAAtttttt                                               >  1:305185/1‑141 (MQ=255)
                                                                                 tattttattACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAatt                                        >  1:86764/1‑141 (MQ=255)
                                                                                      tattaCTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTAtttt                                   >  1:259711/1‑141 (MQ=255)
                                                                                       attaCTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTa                                  >  1:24465/1‑141 (MQ=255)
                                                                                        ttaCTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATtataa                                 >  2:373406/1‑140 (MQ=255)
                                                                                         taCTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGt                                >  1:162124/1‑141 (MQ=255)
                                                                                            tCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGc                             <  1:263154/141‑1 (MQ=255)
                                                                                                    aaatttGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTCTTTTTTTGTAAATTATTTTAGTTGCAAGTTTTg                     >  1:175011/1‑141 (MQ=255)
                                                                                                     aatttGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTgg                    >  2:335398/1‑141 (MQ=255)
                                                                                                     aatttGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTgg                    >  1:372833/1‑141 (MQ=255)
                                                                                                       tttGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGt                  >  2:178112/1‑141 (MQ=255)
                                                                                                       tttGTCTATTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCACTTTTTTGGt                  >  1:234272/1‑141 (MQ=255)
                                                                                                                     aaCATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAAttt    <  1:208589/141‑1 (MQ=255)
                                                                                                                       cATTTTATCTTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTgg  <  1:146189/141‑1 (MQ=255)
                                                                                                                       cATTTTATATTTCATCGTTGTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTgg  <  1:237886/141‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
ATTGCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTACATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTGG  >  CP000730/2337833‑2338092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A