Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F67 I0 R1	54	74.6	1619566	95.7%	1549924	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,233,504	T→A	100%	V470D (GTT→GAT)	USA300HOU_1157 →	non‑specific serine/threonine protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,233,504	0	T	A	100.0%	173.7 / NA	60	V470D (GTT→GAT)	USA300HOU_1157	non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/40); total (20/40)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTA  >  CP000730/1233366‑1233639

cTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATa                                                                                                                                       >  1:782314/1‑141 (MQ=255)

cTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCATGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCCTTAGGTCTTAAAGATGATa                                                                                                                                       >  2:616025/1‑141 (MQ=255)

 tGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATAc                                                                                                                                      >  1:565848/1‑141 (MQ=255)

  ggtgaacgtggtgaacgtGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACg                                                                                                                                     <  1:674804/141‑1 (MQ=255)

    tGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAt                                                                                                                                   <  1:607980/141‑1 (MQ=255)

     gAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAtt                                                                                                                                  <  1:272871/141‑1 (MQ=255)

     gAACGTGTTGAACGTGGTGACAGTGCTGATGTTGTTATATCAAAGGGCCCTGAAAAGCACAAAAATCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAtt                                                                                                                                  <  1:385902/141‑1 (MQ=255)

     gAACGTATGGTACGTGGTGACAGTATCGCTGTCGTTATCTCACAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAtt                                                                                                                                  <  1:469198/141‑1 (MQ=255)

       aCGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCCAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGa                                                                                                                                >  1:86938/1‑141 (MQ=255)

       aCGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGa                                                                                                                                >  1:114228/1‑141 (MQ=255)

       aCGTGTTGAACGTGGTGACAGTGTCGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGa                                                                                                                                >  1:590487/1‑141 (MQ=255)

        cGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGaa                                                                                                                               >  2:334889/1‑141 (MQ=255)

            ttGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAACATGCCACATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAg                                                                                                                           >  2:120507/1‑141 (MQ=255)

                           gtgtTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGGCATTGGTTTACCTCAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAATATATAATAATCaaa                                                                                                            >  1:39504/1‑140 (MQ=255)

                                      gttATTTCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGa                                                                                                 <  2:752148/141‑1 (MQ=255)

                                        tataTCAAAGGGCCCGGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATAAAGCGCCAAAAGGATa                                                                                               <  1:404443/141‑1 (MQ=255)

                                             cAAAGGGCCCTGAAAATGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGACAAAAGGATACATTg                                                                                          <  1:599487/141‑1 (MQ=255)

                                                aGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCaa                                                                                       >  1:798844/1‑141 (MQ=255)

                                                aGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCaa                                                                                       >  1:331649/1‑141 (MQ=255)

                                                    cccTGAAACGGCTAAAATGCCAAATGTCATTCGTTTACCTAAGGAGGAAGACTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAACAATCACGCGAGGCAACTATACATTGCAAGTCa                                                                                   >  2:769461/1‑141 (MQ=255)

                                                    cccGGAAAAGGTTAAAAGGCCAAATTGCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCCTAACGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCa                                                                                   <  1:417228/141‑1 (MQ=255)

                                                     ccTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaa                                                                                  >  1:397353/1‑141 (MQ=255)

                                                     ccTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaa                                                                                  >  1:669398/1‑141 (MQ=255)

                                                                   aaTGCCAAATGTCATTGGTTTACCTACGAAAGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCaaa                                                                    <  2:771862/141‑1 (MQ=255)

                                                                    aggCCAAATGTCATTGGTGTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAAt                                                                   <  1:732598/139‑1 (MQ=255)

                                                                        cAAATGTCATTGGTTTACCTAAGGAGGAAGCATTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTg                                                               <  2:265968/141‑1 (MQ=255)

                                                                        aaaaTGTCATTGGTTTACCTAAGGAGGGAGCCTCGCACAAATTAAAATCGTTAGGGCTTAAAGATGATACGATTGAAAAAGTATATAATAAGCAAGCGCCAAAAGGATACATTGCAAATCCAGGTCTAACGGCAAATAccg                                                               <  2:167755/140‑3 (MQ=255)

                                                                          aaTGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGaa                                                             <  2:565848/141‑1 (MQ=255)

                                                                               cATTGGTTTACCTAAGGAGGAATCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGc                                                        <  2:191710/141‑1 (MQ=255)

                                                                               cATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGc                                                        <  2:79521/141‑1 (MQ=255)

                                                                               cATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGc                                                        <  2:360997/141‑1 (MQ=255)

                                                                                         ccTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCGAATACTGAAATCGCTATTCATGAt                                              <  1:590583/141‑1 (MQ=255)

                                                                                         ccTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAt                                              >  1:668337/1‑141 (MQ=255)

                                                                                         ccTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAt                                              >  2:762739/1‑141 (MQ=255)

                                                                                           tAAGGAGGAAGCCTTGCAGAAATTAAAATCTTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTc                                            <  1:109072/141‑1 (MQ=255)

                                                                                           tAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTc                                            <  1:117592/141‑1 (MQ=255)

                                                                                           tAAGGAGGAAGCCTTGCAGAAATTAAAAGCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTc                                            <  1:336616/141‑1 (MQ=255)

                                                                                             aggaggAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTa                                          <  1:529508/141‑1 (MQ=255)

                                                                                             aggaggAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAAGACTGAAACCGCTATTCATGATtata                                          <  2:782314/141‑1 (MQ=255)

                                                                                             aggagcAAGCCGTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAGAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTa                                          <  1:616025/141‑1 (MQ=255)

                                                                                                gggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAata                                       <  2:53650/140‑1 (MQ=255)

                                                                                                aggaAGCCATGCAGAAATTAAAATCGTTAGGTCTTAAAGAAAAAAAAAATAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAata                                       <  2:667628/141‑1 (MQ=255)

                                                                                                  caaGCCTTGCAGGAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAGTAtt                                     <  2:492428/140‑1 (MQ=255)

                                                                                                     gCCTTGCAGAAATTAAAAGCGTTAGGTCTTAAAGATGATACGCTTGAAATGGTATCTAATAATCAAGCGCCAAAAGGATACATTTCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTaaa                                  <  2:368866/141‑1 (MQ=255)

                                                                                                       cTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCACATACTGAAATCGCTATTCATGATTCTCATATTCAACt                                >  2:32882/1‑141 (MQ=255)

                                                                                                            aGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATg                           <  1:529315/141‑1 (MQ=255)

                                                                                                            aGAAATTAAAATCGTTAGGTCTTAAACATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATg                           <  1:490083/141‑1 (MQ=255)

                                                                                                                agtagaatctgtAGGTGTGAAAGAGGATACGATGGAAAAAGTATATGAGAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGGAACCTCAAATACTGAAATCGCTATTCATGATTCTAATAGTAAACTATATGAATc                       <  1:769461/130‑1 (MQ=255)

                                                                                                                aTTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATc                       >  2:765125/1‑141 (MQ=255)

                                                                                                                   aaaaTCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                    <  2:725119/141‑1 (MQ=255)

                                                                                                                   aaaaTCGTTAGGTCTTAAAGATAAAACAAAAAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                    <  2:470636/141‑1 (MQ=255)

                                                                                                                   aaaaGCGTTACGTCGTAAAGATGACACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                    <  2:264643/141‑1 (MQ=255)

                                                                                                                   aaaaCCGTAAGGTCTAAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                    <  1:407705/141‑1 (MQ=255)

                                                                                                                         gTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCAt              >  1:687195/1‑141 (MQ=255)

                                                                                                                            aGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCACTATTCATGATTATAATATTAAACTATATGAATCTTTAGGCATTaa           >  2:454572/1‑141 (MQ=255)

                                                                                                                             ggTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAg          <  2:792726/141‑1 (MQ=255)

                                                                                                                             agTCATAAACATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAg          <  2:686233/140‑1 (MQ=255)

                                                                                                                              gTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGc         <  2:331649/141‑1 (MQ=255)

                                                                                                                              gTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGc         <  2:798844/141‑1 (MQ=255)

                                                                                                                                     aGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTa  <  1:57394/141‑1 (MQ=255)

CTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTA  >  CP000730/1233366‑1233639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A