Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F52 I0 R1	98	95.8	2129336	92.9%	1978153	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,233,504	T→A	83.1%	V470D (GTT→GAT)	USA300HOU_1157 →	non‑specific serine/threonine protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,233,504	0	T	A	83.1%	207.6 / 33.5	89	V470D (GTT→GAT)	USA300HOU_1157	non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand): ref base T (5/10); new base A (32/41); total (37/52)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

TGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTATGTAG  >  CP000730/1233367‑1233644

tGGTGAACGTGTTGAACGTGGCGACAGTGTTGATGTTGATATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATAc                                                                                                                                           <  1:886313/141‑1 (MQ=255)

  gTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGa                                                                                                                                         <  1:696061/141‑1 (MQ=255)

   tGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAt                                                                                                                                        <  1:1034410/141‑1 (MQ=255)

   tGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAt                                                                                                                                        <  2:876609/141‑1 (MQ=255)

    gAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAtt                                                                                                                                       <  1:99768/141‑1 (MQ=255)

      aCGTGTTGAACGTGGTGATAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGa                                                                                                                                     <  2:63086/141‑1 (MQ=255)

      aCGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACgcaaga                                                                                                                                     >  2:895716/1‑136 (MQ=255)

      aCGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGa                                                                                                                                     >  2:1040469/1‑141 (MQ=255)

         tgtTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGaaaa                                                                                                                                  >  1:157385/1‑141 (MQ=255)

                cGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGtatat                                                                                                                           <  2:245601/141‑1 (MQ=255)

                      gACAGTGTTGATGTTGATATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATAATAAAAAAAAACAAGTATATaataat                                                                                                                     <  2:719749/141‑1 (MQ=255)

                       aCAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATc                                                                                                                    >  1:404212/1‑141 (MQ=255)

                          gtgtTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAg                                                                                                                 <  2:590856/141‑1 (MQ=255)

                           tgtTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGACGATACGATTGAAAAAGTATATAATAATCAAgc                                                                                                                <  2:338395/141‑1 (MQ=255)

                             ttGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAgcgc                                                                                                              >  1:202808/1‑141 (MQ=255)

                              tGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGCTACGATTGAAAAAGTATATAATAATCAAGCGcc                                                                                                             <  2:1050950/141‑1 (MQ=255)

                               gATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCa                                                                                                            >  2:653968/1‑141 (MQ=255)

                                  gttgttATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAAAAATCAAGCGCCaaaa                                                                                                         >  1:971539/1‑141 (MQ=255)

                                   ttgttATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATATTCATGCGCCaaatg                                                                                                        >  2:932282/1‑139 (MQ=255)

                                   ttgttATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCaaaaa                                                                                                        <  2:672480/141‑2 (MQ=255)

                                   gtgttATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAg                                                                                                        <  2:226340/140‑1 (MQ=255)

                                      ttATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGAt                                                                                                     >  1:125505/1‑141 (MQ=255)

                                      ttATATCAAAGGGCCATGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCTAAAGGAt                                                                                                     <  1:662375/141‑1 (MQ=255)

                                        atatCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTCTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTGAAATATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATAc                                                                                                   <  1:508645/141‑1 (MQ=255)

                                           tCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACAtt                                                                                                >  1:232471/1‑141 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGTTAACATGCCAAATGTCTTTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGTAAAAGTATATAATAATCAACAGCGAAAAGGATACAtta                                                                                               >  2:164762/1‑140 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGTTAAAATGCCACGTCTCACATGCGTACCTCCAGAGGGCGGAAATCGGAAATTAAAAGCGGTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTg                                                                                               >  2:1019098/1‑141 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTACAGATGAGACGATTGAAATAGTATACAATAATCAAGCGCCAAAAGGGTACATTg                                                                                               <  1:672032/141‑1 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTg                                                                                               <  1:790764/141‑1 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACAAAAAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTg                                                                                               <  2:132520/141‑1 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGTTAAAATCCCCCCTGCCCTTTGGTTAACTAAGGCGTAATCCCTGCAGAAACTAAAATCGCCAGGTCTTAAAGATGACACGATTGAAAAAGTATATACTAATCAAGCGCCAAAAGGACCCAttc                                                                                               >  2:1019218/1‑140 (MQ=255)

                                            cAAAGGGCCCTGAAAAGGATAAAAAGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGTTCTTAAAGATAAAAAAAATAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTg                                                                                               <  2:458990/141‑1 (MQ=255)

                                            cAAAGGGACCTGAAAACGATAAAATGACAAATGACATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGAAAAAAAAAAAAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTg                                                                                               <  2:1035583/141‑1 (MQ=255)

                                             aaaGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGc                                                                                              >  1:1030544/1‑141 (MQ=255)

                                             aaaGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGc                                                                                              >  1:1030581/1‑141 (MQ=255)

                                              aaGGGCCTTGAAAAGGTTAAAATGCCAAATGTCATTTGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCa                                                                                             <  1:74935/141‑1 (MQ=255)

                                                gggCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGCTAGGTCTTAAAGATGAAACGATCGAAAAAGCATATAATAATCAAGCGCCAAAAGAATACATAGCaaa                                                                                           >  2:28125/1‑141 (MQ=255)

                                                    ccTGAAAAGGTTAAAAGGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaa                                                                                       >  1:30868/1‑141 (MQ=255)

                                                     cTGAAAAGGTTAAACTGCCAAATGTCATGGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTgcaaagcaaa                                                                                      >  1:691394/1‑141 (MQ=255)

                                                     cTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaaa                                                                                      >  2:988408/1‑141 (MQ=255)

                                                     cTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaaa                                                                                      >  2:545095/1‑141 (MQ=255)

                                                     cTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAAACaaa                                                                                      >  2:20656/1‑141 (MQ=255)

                                                      tGAAAAGGTTAAAATTCTAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGCTACATTGCAAATCaaaa                                                                                     >  2:757277/1‑140 (MQ=255)

                                                      tGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAg                                                                                     <  2:280252/141‑1 (MQ=255)

                                                      tGAAAAGGTTAAAAGGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAg                                                                                     <  2:88689/141‑1 (MQ=255)

                                                       gAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAgt                                                                                    >  1:985998/1‑141 (MQ=255)

                                                       gAAAAGGTTAAAATGCCAAATGTCAGTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAgt                                                                                    <  2:603084/141‑1 (MQ=255)

                                                            ggTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAAc                                                                               <  1:541186/141‑1 (MQ=255)

                                                            ggTCAAAATGCCAAATGACATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATAAAAAAAATAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAAc                                                                               <  2:746451/141‑1 (MQ=255)

                                                              ttAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCTTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCg                                                                             <  2:540931/141‑1 (MQ=255)

                                                               tAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGc                                                                            <  1:517286/141‑1 (MQ=255)

                                                                      ccAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCGGAAATGAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACGGCAAAAACt                                                                     >  2:529710/1‑141 (MQ=255)

                                                                      ccAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTACAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACt                                                                     >  2:852791/1‑141 (MQ=255)

                                                                      ccAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACt                                                                     >  1:193700/1‑141 (MQ=255)

                                                                      ccAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACt                                                                     <  1:626373/141‑1 (MQ=255)

                                                                      ccAAATGTCATTGGATTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACt                                                                     >  1:556079/1‑141 (MQ=255)

                                                                        aaaaGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAATTACTGa                                                                   <  2:666584/141‑1 (MQ=255)

                                                                           tgataTTGGTTTACCTAAGGAGCAAGCCGTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAAGCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAAt                                                                <  1:603530/137‑1 (MQ=255)

                                                                           tGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAAt                                                                <  1:271068/141‑1 (MQ=255)

                                                                                 tGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTAt                                                          <  1:953178/141‑1 (MQ=255)

                                                                                  ggTTTACCTAAGGAGGAAGCCTTGCAGAAAATAAAATCGTTAGGTCTTAAAGAAAAAAAAAAAAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTAtt                                                         <  2:1033349/141‑1 (MQ=255)

                                                                                  ggTTGACCTAATGATGAAGCCTTGCAGAAATTAAAATCGTTAGGTATCAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTAtt                                                         <  1:54659/141‑1 (MQ=255)

                                                                                   gTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTc                                                        <  1:318642/141‑1 (MQ=255)

                                                                                    tttACCTAAGGAGGAAGCCTGGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCa                                                       <  2:533904/141‑1 (MQ=255)

                                                                                         cTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGCTTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTACTGAtt                                                  >  2:692025/1‑141 (MQ=255)

                                                                                           aaGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCt                                                <  1:22853/141‑1 (MQ=255)

                                                                                            aggaggAAGCCTTGCAGAAGTTAAAATCGTTAGGTCATAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTa                                               >  1:561967/1‑141 (MQ=255)

                                                                                             ggaggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCTAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTaa                                              <  2:404212/141‑1 (MQ=255)

                                                                                              gaggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAat                                             >  1:651275/1‑141 (MQ=255)

                                                                                              gaggaAGCCTTGCAGAAATTAAAATCGTTAAGTATTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAATGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAat                                             <  2:669992/141‑1 (MQ=255)

                                                                                               aggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCACCCGCCTTAATGTAAGAGAGGAATGCGAAGTGAACTAATGAATACTGAAATCGCTATTCATGTTTCTAata                                            <  2:748122/141‑1 (MQ=255)

                                                                                               aggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAata                                            <  2:913644/141‑1 (MQ=255)

                                                                                                ggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAatat                                           <  2:2898/141‑1 (MQ=255)

                                                                                                    gCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTaaa                                       >  1:101338/1‑141 (MQ=255)

                                                                                                    gCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTaaa                                       >  1:658161/1‑141 (MQ=255)

                                                                                                          cAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTatat                                 <  1:413368/141‑1 (MQ=255)

                                                                                                          cAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTatat                                 <  1:93868/141‑1 (MQ=255)

                                                                                                                  aggaTCGTTAGGTCTTGAAGATGATACGATTGAAAGAGTATATAGTAGTCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                         <  2:290587/138‑1 (MQ=255)

                                                                                                                  aaaaTCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                         <  1:862334/141‑1 (MQ=255)

                                                                                                                  aaaaTCGTAAGGCTTTAAAGCTGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCttt                         <  2:971539/141‑1 (MQ=255)

                                                                                                                    aaTCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAg                       >  1:204037/1‑141 (MQ=255)

                                                                                                                    aaTCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAg                       <  2:1035129/141‑1 (MQ=255)

                                                                                                                     aTCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAGTATTAAACTATATGAATCTTTAgg                      >  2:663308/1‑141 (MQ=255)

                                                                                                                      tCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAGTCTTTAGGc                     >  2:369111/1‑141 (MQ=255)

                                                                                                                        gTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATAGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTGAGGCAt                   >  1:96795/1‑141 (MQ=255)

                                                                                                                          tAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTa                 >  2:1012778/1‑141 (MQ=255)

                                                                                                                          tAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTa                 <  1:658546/141‑1 (MQ=255)

                                                                                                                                  aaaGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGtt         <  1:624060/141‑1 (MQ=255)

                                                                                                                                  aaaGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGtt         <  1:797896/141‑1 (MQ=255)

                                                                                                                                     gatgatACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTAt      >  2:519623/1‑141 (MQ=255)

                                                                                                                                     gatgatACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACAGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTAt      >  1:715401/1‑141 (MQ=255)

                                                                                                                                         ttACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATATTTAGGCATTAAGCAAGTTTATGTag  >  2:121427/1‑141 (MQ=255)

TGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTATGTAG  >  CP000730/1233367‑1233644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A