breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 4,449 C→A 6.2% Q171K (CAA→AAA)  recF → recombination protein RecF
RA 35,814 Δ1 bp 34.9% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,817 A→T 36.5% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,850 C→T 42.6% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,858 T→A 41.7% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,876 C→T 46.3% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,913 C→G 23.6% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase
RA 35,940 G→A 27.0% R204R (CGC→CGT USA300HOU_0026 ← IS431mec transposase
RA 35,970 T→C 59.8% E194E (GAA→GAG USA300HOU_0026 ← IS431mec transposase
RA 35,997 T→C 30.6% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase
RA 36,015 C→T 31.1% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,087 A→G 58.7% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase
RA 36,119 C→T 28.5% V145I (GTA→ATA)  USA300HOU_0026 ← IS431mec transposase
RA 36,124 G→A 57.0% A143V (GCT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,133 A→G 29.6% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,161 T→C 30.0% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,174 T→C 30.2% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase
RA 36,269 C→T 58.9% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase
RA 36,315 G→A 54.6% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase
RA 36,378 C→T 85.3% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase
RA 36,413 C→T 51.8% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase
RA 36,444 C→T 47.9% R36R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,471 A→G 32.4% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase
RA 36,479 T→C 22.9% T25A (ACA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,501 G→A 4.4% A17A (GCC→GCT USA300HOU_0026 ← IS431mec transposase
RA 36,581 C→T 35.0% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 76,180 C→T 27.4% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 11.5% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 33.8% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 53.2% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 53.5% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 49.9% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,969 G→A 66.0% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,651 C→T 6.8% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 10.4% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 27.2% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 26.1% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 10.6% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 162,116 G→A 8.5% A180V (GCA→GTA)  USA300HOU_0153 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 195,286 T→A 87.1% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 215,097 T→C 5.2% K164E (AAA→GAA)  argC ← N‑acetyl‑gamma‑glutamyl‑phosphate reductase
RA 241,553 T→C 4.3% G187G (GGT→GGC ggt → T3 family gamma‑glutamyltransferase
RA 396,540 G→A 85.9% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 405,184 C→A 5.5% W61L (TGG→TTG)  USA300HOU_0374 ← possible bacterial low temperature requirement protein A
RA 434,310 A→T 7.4% L72* (TTA→TAA)  USA300HOU_0408 ← hypothetical protein
RA 512,643 A→G 43.3% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 602,466 G→T 9.2% intergenic (+56/‑101) USA300HOU_0544 → / → araB possible hydrolase/ribulokinase
RA 607,884 G→A 89.8% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase
RA 629,681 C→A 71.2% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 84.4% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase
RA 707,910 A→G 83.4% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 863,781 A→G 5.9% intergenic (+310/‑30) eno → / → USA300HOU_0807 phosphopyruvate hydratase/hypothetical membrane protein
RA 951,713 G→T 9.8% intergenic (+218/‑90) USA300HOU_0915 → / → rocD flavin reductase/ornithine‑‑oxo‑acid transaminase
RA 1,231,474 G→A 4.4% G40D (GGT→GAT)  USA300HOU_1156 → possible PP2C protein phosphatase
RA 1,233,504 T→A 83.1% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,265,591 C→T 7.5% intergenic (+99/‑74) fmhC → / → USA300HOU_1180 FemAB family peptidoglycan biosynthesis protein/SMF family nucleotide‑binding protein
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T 89.2% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,646,545 G→A 6.3% T36T (ACC→ACT USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,744,362 G→A 92.4% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 78.6% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,821,268 C→T 9.0% M257I (ATG→ATA ald ← alanine dehydrogenase
RA 1,929,796 T→C 30.2% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 30.1% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 30.1% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 31.0% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 46.6% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 51.6% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 53.5% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 55.1% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,863:1 +T 39.6% intergenic (‑946/‑222) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,864 A→T 14.8% intergenic (‑947/‑221) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 40.0% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865:1 +C 8.5% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 51.3% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 53.8% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 54.8% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 54.4% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 54.2% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 55.1% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 47.4% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 47.0% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 46.7% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 46.0% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 46.2% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 41.5% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 30.4% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,963,768 C→G 90.3% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,211,273 A→G 9.0% M1T (ATG→ACG) † USA300HOU_2086 ← hypothetical protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,352,815 G→A 84.5% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,454,626 A→T 75.1% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,725,352 A→T 6.2% intergenic (‑58/+276) USA300HOU_2578 ← / ← USA300HOU_2579 hypothetical protein/hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =102 (1.150)66 (1.080)
+40 bp
48/180 NT 48.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA