Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F123 I0 R1	46	52.8	1143652	95.1%	1087613	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,352,815	G→A	100%	A92V (GCA→GTA)	rpsI ←	ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,352,815	0	G	A	100.0%	173.2 / NA	58	A92V (GCA→GTA)	rpsI	ribosomal protein S9
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/26); total (32/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAATT  >  CP000730/2352678‑2352952

tatCATCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACtt                                                                                                                                        >  1:408259/1‑141 (MQ=255)

      ccGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCtaata                                                                                                                                  >  2:119197/1‑141 (MQ=255)

        gACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGATCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTaataat                                                                                                                                >  2:87943/1‑141 (MQ=255)

          cAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGGTCTACTTCTAATGATGc                                                                                                                              >  2:318914/1‑141 (MQ=255)

           aaTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCCGGATCTACTTCTAATAATGcc                                                                                                                             >  1:491758/1‑140 (MQ=255)

              tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGGTCTACTTCTAATAATGCACGa                                                                                                                          >  1:499731/1‑141 (MQ=255)

               taCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAg                                                                                                                         <  1:373313/140‑1 (MQ=255)

                aCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGGTCTACTTCTAATAATGCACGAGc                                                                                                                        >  1:6685/1‑141 (MQ=255)

                   ttttGAGAATTGAGGTGAACGACGAGTTGCTTTAAGACCTGGTTTATTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGAt                                                                                                                     <  1:130446/141‑1 (MQ=255)

                                  tGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagc                                                                                                      <  2:125000/141‑1 (MQ=255)

                                  ggAACGACAAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagc                                                                                                      <  2:11596/140‑1 (MQ=255)

                                        acgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagc                                                                                                <  1:146397/141‑1 (MQ=255)

                                             ctgctTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCTGGATCTACTTCTAGTAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTc                                                                                           >  1:157131/1‑141 (MQ=255)

                                                   taaaaaccgattttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGa                                                                                     <  1:152956/131‑1 (MQ=255)

                                                        ccTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAacca                                                                                <  1:222910/141‑1 (MQ=255)

                                                         cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCGGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccac                                                                               >  1:146795/1‑141 (MQ=255)

                                                            gTTTTTTTCGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccac                                                                            <  2:98878/141‑1 (MQ=255)

                                                            gTTTTTTACTTTTTTTCATACGTGGAAAACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTTACTTATAGCTTGAGCTTGTCCAGTGAAaccaccac                                                                            >  2:511694/1‑141 (MQ=255)

                                                            gTTTTTTACGTTCTTGCATACGTGGGTCACGAGGAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccac                                                                            <  1:178416/141‑1 (MQ=255)

                                                                  tACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGGTTGTCGAGTGAAACCACCACCCTGaa                                                                      >  1:321034/1‑141 (MQ=255)

                                                                   aCGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTACAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCAATTCCGTGACGGATAGCTTGTGCTTGTCCAGAGACACCACCACCACGaaa                                                                     >  2:373181/1‑140 (MQ=255)

                                                                     gTTCTTTCATCCGTGGGCCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATCCAGGAGCTACTTCCAATAATGCACGAGCGATTCCGTGACGGATAGCTCGACCTTGTCCAGTGCACCCCACACCCTGAACGt                                                                   >  2:127109/1‑141 (MQ=255)

                                                                     gTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGACCCTCTGTATTCAGGCTCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGacgct                                                                   >  2:18243/1‑137 (MQ=255)

                                                                     gTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGGTCTACTTCTACTAATGCACGGGCGATTCCGTGGCGGATTACTTGAGGTTGTCCAGTGAAACCCCCACCATGAACGt                                                                   >  2:324013/1‑141 (MQ=255)

                                                                      ttCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCAACAtcctcgtt                                                                  <  2:493199/141‑8 (MQ=255)

                                                                          ttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACt                                                              >  1:29299/1‑141 (MQ=255)

                                                                            cATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaa                                                            <  2:464191/141‑1 (MQ=255)

                                                                             aTACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCCCCACCATGAACGTTAACTaaa                                                           >  2:71381/1‑141 (MQ=255)

                                                                              tACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCCTGAACGTTAACTaaaa                                                          >  2:152930/1‑141 (MQ=255)

                                                                               aCGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTGGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAc                                                         >  2:55197/1‑141 (MQ=255)

                                                                                  tGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatc                                                      >  1:224182/1‑141 (MQ=255)

                                                                                         cGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATCGCTTGAGCTTGTCCAGTGAAACCACCCCCATGAACGTTAACTAAAACATCATAGTTa                                               >  2:174049/1‑141 (MQ=255)

                                                                                             taagtaaTCCAGCGCGTTTTAAACAACCTCTGTATTCAAGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATTAACGTTAACTAAAACATCATAGTTACCtt                                           <  1:64809/141‑1 (MQ=255)

                                                                                               agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTACCTAAAACATCATAGTTACCTTTa                                         >  1:125712/1‑141 (MQ=255)

                                                                                               agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATCGTTACCTTTa                                         >  1:232840/1‑141 (MQ=255)

                                                                                                 taaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGt                                       <  1:100293/141‑1 (MQ=255)

                                                                                                 taaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCAAGAGCGATTCCGTGACGGATAGATTGAGCTTGTACAGTCAAACGACCACCATGAACGTTAACTAAAACATCATAGTTACATCTAGt                                       >  2:545404/1‑141 (MQ=255)

                                                                                                      caacgcgTTTTAAAGTACCTCTGTAAGCAGAAACTACTTCTAATAATGAACGAGCGATTCCGTGACGGATAGCTTGAGCTTGGCCAGTGAAACCACCACCATGAACGCTAACTAAAACATCATGGTTCCCGTTCGTCTCAg                                  >  2:431887/4‑141 (MQ=255)

                                                                                                      cAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAg                                  >  2:527195/1‑141 (MQ=255)

                                                                                                        gcgcGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGtt                                >  2:305979/1‑141 (MQ=255)

                                                                                                             ttttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                           <  1:232091/141‑1 (MQ=255)

                                                                                                             ttttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                           <  1:231846/141‑1 (MQ=255)

                                                                                                             ttttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                           <  1:119197/141‑1 (MQ=255)

                                                                                                             ttttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                           >  1:264516/1‑141 (MQ=255)

                                                                                                             ttttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                           >  1:264698/1‑141 (MQ=255)

                                                                                                              tttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCa                          >  1:92652/1‑141 (MQ=255)

                                                                                                              tttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCa                          >  1:519900/1‑141 (MQ=255)

                                                                                                               ttAAATAATCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaa                         <  2:108643/141‑1 (MQ=255)

                                                                                                               ttAAAGAACCTCTGTATTCAGGATCTACGTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaa                         <  1:264530/141‑1 (MQ=255)

                                                                                                                tAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaaa                        <  1:464068/141‑1 (MQ=255)

                                                                                                                   aGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACCTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtgg                     <  2:370180/141‑1 (MQ=255)

                                                                                                                     aaCCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggtt                   <  1:511484/141‑1 (MQ=255)

                                                                                                                       ccTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACTTCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttgg                 <  2:286600/141‑1 (MQ=255)

                                                                                                                       ccTCTGCATTCAGGATCCACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATAATAGTTACCTTTAGTTTCAGTTACATCAAAtggttgg                 <  2:74641/141‑1 (MQ=255)

                                                                                                                               ttCAGGCTCTACTTCTAATAATGCACGAGCAATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTc         <  2:479168/141‑1 (MQ=255)

                                                                                                                               ttCAGGATCGATTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGATCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTc         <  2:187114/141‑1 (MQ=255)

                                                                                                                                 cAGGATCTACTTCTAACAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATTAGATGGTTGGTTTAGGTCTa       >  1:284382/1‑141 (MQ=255)

                                                                                                                                      tCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAAtt  <  2:433878/141‑1 (MQ=255)

TATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAATT  >  CP000730/2352678‑2352952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A