Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F58 I1 R2	17	42.8	915862	63.3%	579740	150.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,289,257	A→C	V78G (GTA→GGA)	ybbP ←	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,289,257	0	A	C	97.1%	112.1 / NA	35	V78G (GTA→GGA)	ybbP	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (11/23); total (11/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAG  >  CP000730/2289110‑2289399

tatTGCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATcccc                                                                                                                                             >  2:214342/1‑151 (MQ=255)

    gCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCa                                                                                                                                         >  1:120934/1‑151 (MQ=255)

    gCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCGACCACGACGATTGCGGTCTAACGCACTTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCa                                                                                                                                         >  1:270873/1‑151 (MQ=255)

      acaGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCAtt                                                                                                                                       <  2:277631/151‑1 (MQ=255)

       caGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTg                                                                                                                                      >  2:199023/1‑151 (MQ=255)

             ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAAc                                                                                                                                <  1:57576/151‑1 (MQ=255)

             ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAAc                                                                                                                                <  1:92782/151‑1 (MQ=255)

                     gATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGa                                                                                                                        >  2:440514/1‑151 (MQ=255)

                       ttGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGaat                                                                                                                      <  2:303553/151‑1 (MQ=255)

                             cAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTa                                                                                                                >  1:72296/1‑151 (MQ=255)

                                tttCTTTTCATTATTAATATAAGTATTAGAAGTATAGCGTTATAAAAAGCTACCTCTACCAAGTTGTTCTAACGCAAATCTAATTTCTTGTTAGAATATTAATATTAAAGTTAATAACACCAATTGAATAACGATATCGAATAATTTAGAt                                                                                                             <  2:92733/151‑1 (MQ=255)

                                   ctctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGtt                                                                                                          >  2:412542/1‑151 (MQ=255)

                                          tCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCa                                                                                                   >  2:235790/1‑151 (MQ=255)

                                          tCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCa                                                                                                   <  2:120934/151‑1 (MQ=255)

                                           cTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCaa                                                                                                  >  1:387860/1‑151 (MQ=255)

                                            tttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAg                                                                                                 <  1:425849/151‑1 (MQ=255)

                                            tttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAg                                                                                                 <  2:113937/151‑1 (MQ=255)

                                              tACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGtt                                                                                               <  1:358301/151‑1 (MQ=255)

                                              tACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGtt                                                                                               <  1:358323/151‑1 (MQ=255)

                                                     cGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATc                                                                                        >  2:74734/1‑151 (MQ=255)

                                                      gTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCGCCCCCATTGAATAACGATATCGACTAATTTAGATGTTGCAGTCAAGTTCAATATCa                                                                                       <  2:388563/151‑1 (MQ=255)

                                                       tATTAGAAGTATAGCGTTTTAAAAAGCTAATTCTACCAAGTTGTTCTAACGAACGTCTAATTTCTGGTTGGACTATTACTATTAAAGCTCATCCCCCCCATTGAATAACGATTTCGAATAATTTAGATGTTGAAATGAAGTTCAATATCAt                                                                                      <  2:193116/151‑1 (MQ=255)

                                                                      gTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGCATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGAcc                                                                       <  2:865/151‑1 (MQ=255)

                                                                        tttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCaa                                                                     <  2:37223/151‑1 (MQ=255)

                                                                            aaaaGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGCTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAAc                                                                 <  2:266218/151‑1 (MQ=255)

                                                                                     ctctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTaa                                                        <  2:14476/151‑1 (MQ=255)

                                                                                          ccAAGTTGTTCTAACGCACGTCTAATNTCTGGTTGNAATNTTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATcc                                                   >  1:421020/1‑151 (MQ=255)

                                                                                             aGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCtt                                                <  1:262155/151‑1 (MQ=255)

                                                                                              gttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCttt                                               <  1:62886/151‑1 (MQ=255)

                                                                                                   tCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTa                                          >  1:194105/1‑151 (MQ=255)

                                                                                                               cTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCtt                              <  1:396510/151‑1 (MQ=255)

                                                                                                                tAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                             <  1:412542/151‑1 (MQ=255)

                                                                                                                 aaTTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTa                            <  2:171161/151‑1 (MQ=255)

                                                                                                                     tCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTc                        <  2:320477/151‑1 (MQ=255)

                                                                                                                        ggTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCt                     <  2:419783/151‑1 (MQ=255)

                                                                                                                                           aaGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGagaag  <  1:301484/151‑1 (MQ=255)

TATTGCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAG  >  CP000730/2289110‑2289399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A