Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F191 I0 R1 47 68.0 1456600 97.2% 1415815 140.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 479,553 G→A 100% A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730479,5530GA100.0% 267.7 / ‑6.5 91A194T (GCA→ACA) USA300HOU_0458hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (43/46);  minor base T (0/2);  total (43/48)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAC  >  CP000730/479417‑479687
                                                                                                                                        |                                                                                                                                      
tGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAAAGCCGAAGCGTGAGCAAAGTTTTTACcatacat                                                                                                                                     >  2:407191/1‑140 (MQ=255)
  aTGCAATAATAGACGAACAAGGTGAGCCACCTAGCGAACAAGTGAATAGACAAATGATTAAATGGACGAAACTTTATATCGATCAGTTTCTAGCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATgg                                                                                                                                   <  2:474218/140‑1 (MQ=255)
   tGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGt                                                                                                                                  <  1:613049/140‑1 (MQ=255)
    gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATggat                                                                                                                                 >  1:281726/1‑138 (MQ=255)
    gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATggat                                                                                                                                 >  1:157901/1‑138 (MQ=255)
        taataGATGAACAAGTTGAGCCAATTAGCTAACAAGTGAATCGTCTGTTACATAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAt                                                                                                                             <  1:710432/140‑1 (MQ=255)
         aataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGCGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAtt                                                                                                                            <  2:201849/140‑1 (MQ=255)
         aacaGATGAACAAGGTGAGCCACTTAGCGAACAAGTGCAGTGCGAAATTAGGAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAtt                                                                                                                            <  2:509814/137‑1 (MQ=255)
            aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGACGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAg                                                                                                                         >  2:113316/1‑140 (MQ=255)
            aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAg                                                                                                                         >  2:213345/1‑140 (MQ=255)
             gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc                                                                                                                        <  1:465960/140‑1 (MQ=255)
             gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCAAAGCGTAAGCAAAGATTTTACCATACATGGTTGCATTTAgc                                                                                                                        >  1:131325/1‑140 (MQ=255)
             gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATACCAAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTatc                                                                                                                        >  1:1284/1‑138 (MQ=255)
              aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcg                                                                                                                       <  1:210524/140‑1 (MQ=255)
                     aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATg                                                                                                                >  2:449781/1‑140 (MQ=255)
                     aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATg                                                                                                                >  2:707084/1‑140 (MQ=255)
                     aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGGTGGACAATACCGAAGCATGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATg                                                                                                                >  1:581199/1‑140 (MQ=255)
                      ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                               >  1:588425/1‑140 (MQ=255)
                      ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACCACACTGGATATCGATCAATTTCTCTCGAGTTGGACCATGACGAAACGTGCGCCAAGTTTTTACCATACATGGTTGCATTTAGCGCacaatca                                                                                                               >  1:646029/1‑134 (MQ=255)
                         gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTCCCATACATGGTTGCATTTAGCGCAACAAGACCa                                                                                                            >  2:176045/1‑140 (MQ=255)
                         gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCa                                                                                                            <  2:228069/140‑1 (MQ=255)
                         gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGACTTGGACAATGCCGAAGCGTGAGCAACGTTTTTCCCATTAACTTTTGCATTTAGCGCAACGTGACCa                                                                                                            <  1:534489/140‑1 (MQ=255)
                         gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCAATCAATTTCTATCGAGTTGAACAATGCCGAAGCGTGAGAAAAGTTTTTACCATACATGGTTGCATGTAGCGCAACATGACCa                                                                                                            >  1:301493/1‑140 (MQ=255)
                          aGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAt                                                                                                           <  2:642313/140‑1 (MQ=255)
                           gCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATa                                                                                                          >  2:620208/1‑140 (MQ=255)
                           gCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATAAATGGTTGCATTTAGCGCAACATGACAAtc                                                                                                          >  2:671181/1‑139 (MQ=255)
                               cTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGttt                                                                                                      >  1:667182/1‑140 (MQ=255)
                               cTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGttt                                                                                                      >  2:545197/1‑140 (MQ=255)
                               cTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGAAGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAATTACCGCAACATGACCTTAGttt                                                                                                      >  2:597996/1‑140 (MQ=255)
                                 tcgcgcACAAGCGAATCGTCAAATTTGCAAATGTACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTCTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTa                                                                                                    >  2:65825/3‑140 (MQ=255)
                                     gAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTaa                                                                                                >  1:125389/1‑140 (MQ=255)
                                          aGTGATTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGcac                                                                                           <  2:229868/140‑1 (MQ=255)
                                             gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAATCACAgc                                                                                        <  2:561336/140‑1 (MQ=255)
                                             gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgc                                                                                        >  1:696361/1‑140 (MQ=255)
                                             gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgc                                                                                        <  1:141996/140‑1 (MQ=255)
                                              aaTCGTCAAATGATTAAATGGGCGAAACTCTATATCGATCAATTTCTATCGAGTTGGACAATGCGGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg                                                                                       >  2:367383/1‑140 (MQ=255)
                                               aTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcgc                                                                                      <  2:459234/140‑1 (MQ=255)
                                               aTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcgc                                                                                      <  2:227263/140‑1 (MQ=255)
                                               aTCGTCAAATAATCAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcgc                                                                                      <  2:292047/140‑1 (MQ=255)
                                                  gtaaaaTCATTAAATTTACAAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCCAAGCGTGAGCAAAGTTTCTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCaa                                                                                   <  2:33687/137‑1 (MQ=255)
                                                  gTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGAGAATGCCGAAGCGTGAACAAAGTTTTTACCAAACATGATTGCATTTAAAGCAACATGACCACAGGTTTACTAAAGAACAGCGACaa                                                                                   >  1:585033/1‑140 (MQ=255)
                                                   tCAAATGATTAAATGGACGAGACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCACAGTTTTACTAAAGCACAGCGCCAAg                                                                                  >  1:439470/1‑140 (MQ=255)
                                                   tCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATAACCATAGTTTTACTAAAGCACAGCGCCAAg                                                                                  >  1:690638/1‑140 (MQ=255)
                                                    cAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGt                                                                                 <  2:562414/140‑1 (MQ=255)
                                                    cAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGt                                                                                 <  2:356290/140‑1 (MQ=255)
                                                        tGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGAtt                                                                             <  1:476475/140‑1 (MQ=255)
                                                         gATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTa                                                                            <  2:637818/140‑1 (MQ=255)
                                                          aTTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTaa                                                                           <  1:581975/140‑1 (MQ=255)
                                                            tAAATGGACGAAACTTTATATCGATCAATTTCTCTCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGAAAACAACGTAACATGACACTAGTTTTACTAAAGCACAGCGCCAAGTGATTaaac                                                                         >  1:20523/1‑139 (MQ=255)
                                                                     gAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCa                                                                <  1:628785/140‑1 (MQ=255)
                                                                        aCTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATg                                                             <  2:613151/140‑1 (MQ=255)
                                                                        aCTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATg                                                             <  1:483301/140‑1 (MQ=255)
                                                                           ttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACTTGACCATAGTTTTACTAAAGCACAGCGCCAAGTGGTTAAAGGCTTACTCAATGATc                                                          >  1:152275/1‑140 (MQ=255)
                                                                           ttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATc                                                          <  2:125389/140‑1 (MQ=255)
                                                                            tgtatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                         <  1:660847/138‑1 (MQ=255)
                                                                            tataTCGATTAATTTCTATTGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                         <  1:338173/140‑1 (MQ=255)
                                                                            tataTCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCACCAAGTGATTAAAGGCTTACCCAATGATcc                                                         >  1:162236/1‑140 (MQ=255)
                                                                            tataTCGATCAATTTCTATCGAGTTGGAAAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                         >  2:333644/1‑140 (MQ=255)
                                                                             atatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCt                                                        <  1:703944/140‑1 (MQ=255)
                                                                             atatCGACCAAGTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCt                                                        <  1:309244/140‑1 (MQ=255)
                                                                                     cAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGAc                                                <  1:596173/140‑1 (MQ=255)
                                                                                      aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGGATTTAGCGCAACCTGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAACCAATGATCCTGAAATGACg                                               >  2:217461/1‑140 (MQ=255)
                                                                                      aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTTAAATGACg                                               >  2:428335/1‑140 (MQ=255)
                                                                                      aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTGGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACg                                               <  1:533825/140‑1 (MQ=255)
                                                                                       aTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGCTTAAAGGCTTACCCAATGATCCTGAAATGACGa                                              >  1:397686/1‑140 (MQ=255)
                                                                                       aTTTCGATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                              <  2:117459/140‑1 (MQ=255)
                                                                                          tCTATCGAGTTGGACAATGCTGAAGTGTTAGCAAAGTTTTTGACTTTATCGCTTACGTTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATag                                           <  1:710070/140‑1 (MQ=255)
                                                                                          tCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATag                                           <  2:541018/140‑1 (MQ=255)
                                                                                            tATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATagag                                         >  2:196426/1‑140 (MQ=255)
                                                                                            tATCGAGATGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTCAAGCACAGCCCCAAGTGCTTACCGCCCTAGCCAAGGATCCTGAAATGACGATagcg                                         >  1:455143/1‑138 (MQ=255)
                                                                                             aTCGAGTTGGAGAATGCCGAAGCGTGAGCAAAGTTTTGACCATACAGGGTTGCATTTAGCGCAACATGACCATAGTTTGACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGt                                        <  2:120265/140‑1 (MQ=255)
                                                                                             aTCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCCCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATagaga                                        >  1:3006/1‑139 (MQ=255)
                                                                                                     ggACAATGCCGAAGCGTGAGCAATGTTTTTACCATACATGGTTGCATTTAGCGCAACAGGACCATAGTTTTACTAAAGCACAGCGCCAAGTGGTTAAAGGATTACCCAATGATCCTGAAATGCCGATAGAGTCAGTATTa                                >  2:226930/1‑140 (MQ=255)
                                                                                                     ggACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTa                                >  1:99799/1‑140 (MQ=255)
                                                                                                     ggACAATACCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTa                                >  1:457804/1‑140 (MQ=255)
                                                                                                      gACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTaa                               <  1:428110/140‑1 (MQ=255)
                                                                                                       aCAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCACTATTAAc                              >  1:556377/1‑140 (MQ=255)
                                                                                                         aaTGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCCACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTc                            <  2:226914/140‑1 (MQ=255)
                                                                                                         aaTGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTc                            >  1:184127/1‑140 (MQ=255)
                                                                                                            gCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAtt                         <  1:671181/140‑1 (MQ=255)
                                                                                                            gCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAtt                         <  1:620208/140‑1 (MQ=255)
                                                                                                               gAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt                      >  1:658520/1‑140 (MQ=255)
                                                                                                                     tGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAg                >  1:91068/1‑140 (MQ=255)
                                                                                                                      taGCAAAGTTATTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATGAAAGGCTTACCCAATGATCCGGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa               <  2:441890/139‑1 (MQ=255)
                                                                                                                      gAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa               <  2:712520/140‑1 (MQ=255)
                                                                                                                      gAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa               <  2:306202/140‑1 (MQ=255)
                                                                                                                          aaaTTTTTTACCATACATGGATGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAg           <  2:157901/140‑1 (MQ=255)
                                                                                                                          aaaGTTTTTACCATACATGGATGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAg           <  2:281726/140‑1 (MQ=255)
                                                                                                                               ttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGa      <  1:449781/140‑1 (MQ=255)
                                                                                                                               ttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGa      <  1:707084/140‑1 (MQ=255)
                                                                                                                                  tACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTa   <  2:427726/140‑1 (MQ=255)
                                                                                                                                   aCCATACATGGTTGCATTTAGCGCATCATGACCATAGTTTTACTAAAGCTCAGCGCCACGTGATTCGAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCACTATTAACACATTTTACAATAGATCAAGAAGACCAc  >  1:99554/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAC  >  CP000730/479417‑479687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A