Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F121 I0 R2	43	36.5	755504	99.2%	749459	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	225,710	C→G	43.8%	T266R (ACA→AGA)	murQ →	possible sugar phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	225,710	0	C	G	43.8%	18.7 / 64.8	41	T266R (ACA→AGA)	murQ	possible sugar phosphate isomerase
Reads supporting (aligned to +/- strand): ref base C (9/14); new base G (11/7); total (20/21)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

GATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAG  >  CP000730/225583‑225846

gatGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtg                                                                                                                             >  1:278325/1‑141 (MQ=255)

gatGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtg                                                                                                                             <  1:154111/141‑1 (MQ=255)

  tGTAAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTAGCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtgtg                                                                                                                           <  2:126640/141‑1 (MQ=255)

        aGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCAt                                                                                                                     <  2:196548/141‑1 (MQ=255)

                    aaaaCTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGgaaga                                                                                                         >  1:1947/1‑141 (MQ=255)

                       aCTGANCGTCCGTTCAATGCGTATTATTCAAAAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGc                                                                                                      <  1:39401/141‑1 (MQ=255)

                         tGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGcaa                                                                                                    <  1:191995/141‑1 (MQ=255)

                          gATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGcaac                                                                                                   >  2:330831/1‑141 (MQ=255)

                              gACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGa                                                                                               >  2:118825/1‑141 (MQ=255)

                                ccGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACg                                                                                             >  1:259312/1‑141 (MQ=255)

                                ccGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACg                                                                                             <  2:1612/141‑1 (MQ=255)

                                       gTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGttatta                                                                                      <  1:90394/141‑1 (MQ=255)

                                          cGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAAc                                                                                   >  2:60522/1‑141 (MQ=255)

                                               tattCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATgg                                                                              <  1:192852/141‑1 (MQ=255)

                                                attCAAGAAATCTGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGt                                                                             >  2:148534/1‑141 (MQ=255)

                                                 ttCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTg                                                                            >  2:301536/1‑141 (MQ=255)

                                                 ttCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTg                                                                            <  1:220596/141‑1 (MQ=255)

                                                   cAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGAc                                                                          <  1:111546/141‑1 (MQ=255)

                                                              tgCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACg                                                               <  1:178795/141‑1 (MQ=255)

                                                               gCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGa                                                              <  2:53987/141‑1 (MQ=255)

                                                                cTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGaa                                                             >  2:275152/1‑140 (MQ=255)

                                                                     acaTATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATc                                                        <  2:8995/141‑1 (MQ=255)

                                                                          tgatgaAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAgaga                                                   >  1:98518/1‑141 (MQ=255)

                                                                           gatgaAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGAt                                                  >  2:103965/1‑141 (MQ=255)

                                                                           gatgaAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGAt                                                  >  1:43315/1‑141 (MQ=255)

                                                                           gatgaAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGAt                                                  >  2:241439/1‑141 (MQ=255)

                                                                            atgaAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATCTAACAATGGTGACATTGTTAAACGAGCAATCAGAGATa                                                 <  1:200338/141‑1 (MQ=255)

                                                                              gaAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGa                                               >  1:335034/1‑141 (MQ=255)

                                                                                    aTGGAGTTATATCAGGTATGTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAAccc                                         <  2:189284/141‑2 (MQ=255)

                                                                                            atatCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAggg                                 >  1:326860/1‑141 (MQ=255)

                                                                                               tCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGTAATCAGAGATAGACAACCTTAGGAGGGAtt                              <  2:339282/141‑1 (MQ=255)

                                                                                                       cTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGAc                      >  1:353690/1‑141 (MQ=255)

                                                                                                         gggCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCa                    <  2:372380/139‑1 (MQ=255)

                                                                                                          aGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCaa                   >  2:78303/1‑141 (MQ=255)

                                                                                                               gatgTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGaac              <  1:190410/141‑1 (MQ=255)

                                                                                                                atgTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGaaca             <  2:210011/141‑1 (MQ=255)

                                                                                                                 tgtgAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGaacaa            >  1:73832/1‑141 (MQ=255)

                                                                                                                  gtgAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGaacaac           >  2:340810/1‑141 (MQ=255)

                                                                                                                     aaaGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGaacaacaac        <  2:1947/141‑1 (MQ=255)

                                                                                                                      aaGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACt       <  2:173726/141‑1 (MQ=255)

                                                                                                                           gCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTGAAATGACCAAAGAACAACAACTTGCAg  >  1:341497/1‑141 (MQ=255)

GATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAG  >  CP000730/225583‑225846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A