Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F172 I1 R2	29	34.5	745914	96.0%	716077	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,289,252	C→A	A80S (GCT→TCT)	ybbP ←	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,289,252	0	C	A	94.1%	98.1 / ‑0.5	34	A80S (GCT→TCT)	ybbP	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (21/11); total (22/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

CAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCG  >  CP000730/2289117‑2289389

caGCCTTTGAAACCGCTTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGACAATa                                                                                                                                      >  1:325512/1‑141 (MQ=255)

caGCCATTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCCAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGataata                                                                                                                                      <  1:262970/141‑1 (MQ=255)

      ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                <  1:167411/141‑1 (MQ=255)

      ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                >  2:242428/1‑141 (MQ=255)

       tGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  1:279172/1‑141 (MQ=255)

       tGAAACCGATTGAATCAATTTCTCTTCATCTCTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  1:173447/1‑141 (MQ=255)

       cgAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  1:283509/2‑141 (MQ=255)

                       aaTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCg                                                                                                               <  1:107861/141‑1 (MQ=255)

                       aaTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCg                                                                                                               >  1:345527/1‑141 (MQ=255)

                          ttCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGaat                                                                                                            <  1:189497/141‑1 (MQ=255)

                              ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  1:303720/1‑141 (MQ=255)

                              ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        <  1:286684/141‑1 (MQ=255)

                              ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  1:20492/1‑141 (MQ=255)

                                 cATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTaa                                                                                                     >  1:40069/1‑140 (MQ=255)

                                 cATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAg                                                                                                     >  2:185067/1‑141 (MQ=255)

                                                          ataGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCAt                                                                            >  2:127252/1‑141 (MQ=255)

                                                            aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                          <  1:137330/141‑1 (MQ=255)

                                                            aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                          <  1:157268/141‑1 (MQ=255)

                                                              cGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                        >  1:166910/1‑141 (MQ=255)

                                                                ttttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTAt                                                                      <  2:234858/141‑1 (MQ=255)

                                                                                          gttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAACATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAata                                            >  2:220556/1‑141 (MQ=255)

                                                                                           gttctatgCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAatat                                           >  2:271783/8‑141 (MQ=255)

                                                                                             cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTAAATAACGATATCGAATAATTTAGATGTTGCAGTAAAGTTCAATATCATACTTATCTGCTGACCAATGACAATTACTAATATca                                         >  2:328173/1‑140 (MQ=255)

                                                                                                     cGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGt                                 >  1:335712/1‑141 (MQ=255)

                                                                                                           aTTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGt                           <  1:48346/141‑1 (MQ=255)

                                                                                                             ttCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTAt                         >  1:163307/1‑141 (MQ=255)

                                                                                                                tGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                      >  2:313425/1‑141 (MQ=255)

                                                                                                                     ggAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAg                 >  1:225697/1‑141 (MQ=255)

                                                                                                                      gAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGt                <  1:93168/141‑1 (MQ=255)

                                                                                                                        atatTACTATTAAAGAGAATACCCCCCATTGAATAACGATATAGAATCATTTAGATGTTGTAGTCAAGTTCAATATCATTCTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAACTAAGTGTATCGCTTTCGTTc              >  2:17311/1‑141 (MQ=255)

                                                                                                                           ttACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCt           >  1:11850/1‑141 (MQ=255)

                                                                                                                           taaCTATTAAAAAAAATAACCCCCATTGAATAACGATATCGAATAAATTAAATGTTTCATTCTAGATCAATATCATACTTATTTACTTTCCTATAAAAATTACTAATATCCTTTCAGGTAATTTTATTGCTTTAGTTCCCt           >  2:226559/3‑141 (MQ=255)

                                                                                                                              ctgttAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTaa        <  2:319924/138‑1 (MQ=255)

                                                                                                                                  tAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGAc    <  1:8734/141‑1 (MQ=255)

                                                                                                                                    aaGCTAATACCACCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg  <  2:269024/141‑1 (MQ=255)

CAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCG  >  CP000730/2289117‑2289389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A