Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F116 I1 R1	34	38.2	765948	97.1%	743735	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,664,466	G→A	A65T (GCG→ACG)	pat →	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,664,466	0	G	A	100.0%	184.4 / NA	50	A65T (GCG→ACG)	pat	acetyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/29); total (21/29)

ATGCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGTTA  >  CP000730/2664329‑2664608

atgCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCgg                                                                                                                                      <  1:37189/148‑1 (MQ=255)

atgCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCgg                                                                                                                                      <  1:82148/148‑1 (MQ=255)

atgCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCgg                                                                                                                                      <  1:145416/148‑1 (MQ=255)

    cATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCa                                                                                                                                  <  1:251342/148‑1 (MQ=255)

             tactacAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCt                                                                                                                         >  1:165278/1‑148 (MQ=255)

             tactacAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCt                                                                                                                         >  2:11446/1‑148 (MQ=255)

             tactacAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCt                                                                                                                         >  1:165159/1‑148 (MQ=255)

             tactacAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCt                                                                                                                         >  2:228053/1‑148 (MQ=255)

             tactacAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGGCTTAGGGTTTACGACGTTCGGTTCATTTAGACCt                                                                                                                         >  1:133088/1‑148 (MQ=255)

                      tGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCa                                                                                                                <  2:186941/148‑1 (MQ=255)

                      tGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCa                                                                                                                <  1:263196/148‑1 (MQ=255)

                           ataCTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGAGCTTGGCCAGCATACCt                                                                                                           <  1:336273/148‑1 (MQ=255)

                           ataCTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCt                                                                                                           <  2:334873/148‑1 (MQ=255)

                            taCTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCta                                                                                                          <  2:36883/148‑1 (MQ=255)

                                  tGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACa                                                                                                    >  2:50602/1‑148 (MQ=255)

                                      ccACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCg                                                                                                >  1:166131/1‑148 (MQ=255)

                                      ccACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCg                                                                                                >  2:343050/1‑148 (MQ=255)

                                           aaCCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACAt                                                                                           <  1:331546/148‑1 (MQ=255)

                                           aaCCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACAt                                                                                           <  2:337766/148‑1 (MQ=255)

                                           aaCCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACAt                                                                                           <  2:92293/148‑1 (MQ=255)

                                                   aCGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTa                                                                                   >  2:379746/1‑148 (MQ=255)

                                                            tCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGc                                                                          <  2:71833/148‑1 (MQ=255)

                                                                aTGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCttaa                                                                      >  1:16201/1‑146 (MQ=255)

                                                                aTGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCa                                                                      >  1:14414/1‑148 (MQ=255)

                                                                aTGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCa                                                                      >  2:128739/1‑148 (MQ=255)

                                                                aTGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCa                                                                      >  2:146989/1‑148 (MQ=255)

                                                                aTGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTACTCATGTCGATGATTCa                                                                      >  1:213993/1‑148 (MQ=255)

                                                                    tttGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTa                                                                  <  2:24849/148‑1 (MQ=255)

                                                                          acgaaacAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGaa                                                            >  2:81009/1‑148 (MQ=255)

                                                                          acgaaacAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGaa                                                            >  2:141942/1‑148 (MQ=255)

                                                                             aaacAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAg                                                         <  2:311863/148‑1 (MQ=255)

                                                                             aaacAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAg                                                         <  1:195566/148‑1 (MQ=255)

                                                                               acaacGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGt                                                       <  2:213583/148‑1 (MQ=255)

                                                                               acaacGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGt                                                       <  1:370522/148‑1 (MQ=255)

                                                                                    gTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGc                                                  <  1:102744/148‑1 (MQ=255)

                                                                                    gTAAGCATGAGACTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAAAAAAAAAAAAAGGTATTGc                                                  <  2:86342/148‑1 (MQ=255)

                                                                                        gCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGt                                              >  1:65038/1‑148 (MQ=255)

                                                                                             aGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAAtt                                         <  1:109923/148‑1 (MQ=255)

                                                                                              gCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATta                                        >  2:333641/1‑148 (MQ=255)

                                                                                               ccTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATtac                                       <  1:343050/148‑1 (MQ=255)

                                                                                                 tATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATtacta                                     <  1:101274/148‑1 (MQ=255)

                                                                                                 tATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATtacta                                     <  1:341725/148‑1 (MQ=255)

                                                                                                             tGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAAtt                         >  2:240055/1‑148 (MQ=255)

                                                                                                              gAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATtg                        <  2:294301/148‑1 (MQ=255)

                                                                                                              gAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATtg                        <  2:122510/148‑1 (MQ=255)

                                                                                                                       ggAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCaa               <  2:143061/148‑1 (MQ=255)

                                                                                                                       ggAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCaa               <  2:310999/148‑1 (MQ=255)

                                                                                                                                  aGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGt    >  1:18824/1‑148 (MQ=255)

                                                                                                                                    ggTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGTTa  >  1:200245/1‑148 (MQ=255)

                                                                                                                                    ggTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGTTa  <  1:379746/148‑1 (MQ=255)

ATGCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGTTA  >  CP000730/2664329‑2664608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A