breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,378 C→T D586N (GAT→AAT)  mecA ← penicillin binding protein 2 prime
RA 367,644 G→T intergenic (‑96/‑20) nanA ← / → nagC N‑acetylneuraminate lyase/possible transcriptional regulator
RA 406,961 C→G A353G (GCA→GGA)  atoB → acetyl‑CoA C‑acetyltransferase
RA 479,553 G→A A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 638,614 G→T T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 646,838 G→A intergenic (+192/‑395) lplA2 → / → mvaK1 lipoate‑‑protein ligase A/mevalonate kinase
RA 654,252 C→T S362L (TCG→TTG)  USA300HOU_0591 → hypothetical protein
RA 707,879 A→T L11I (TTA→ATA)  mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 790,778 G→A intergenic (+277/‑197) kipA → / → ltaS possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 942,060 C→G intergenic (‑31/+218) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
RA 942,163 (A)6→5 intergenic (‑134/+115) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
RA 1,087,670 A→T L5I (TTA→ATA)  USA300HOU_1021 ← hypothetical membrane protein
RA 1,232,465 G→C A124P (GCG→CCG)  prkC → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,420,434 G→A A229T (GCG→ACG)  oppD → M3 family oligoendopeptidase F
RA 1,440,007 Δ1 bp coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,530,723 G→A T188I (ACA→ATA)  bshA ← glycosyltransferase
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,454 C→T G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,775,556 G→A P323L (CCT→CTT)  clpX ← S14 family endopeptidase ClpX
RA 1,797,772 G→C R7G (CGC→GGC)  phoR ← sensor histidine kinase
RA 1,834,119 G→T intergenic (+68/‑46) ymaD → / → sgaA hypothetical protein/class V aminotransferase
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,027,561 G→C T129S (ACC→AGC)  vraS ← sensor histidine kinase VraS
RA 2,035,886 T→C intergenic (‑212/‑3) USA300HOU_1892 ← / → dnaQ hypothetical protein/DNA‑directed DNA polymerase III
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,664,466 G→A A65T (GCG→ACG)  pat → acetyltransferase
RA 2,679,708 G→A W64* (TGG→TGA cbpD → possible secretory antigen
RA 2,719,269 (A)5→6 coding (213/705 nt) USA300HOU_2573 → dehydrogenase
RA 2,856,087 T→A P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =48 (1.270)27 (0.980)
+40 bp
19/214 0.2 43.6% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA