Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F177 I1 R2	35	38.3	831348	95.5%	793937	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,274,965	G→C	E106D (GAG→GAC)	bglG →	transcriptional antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,274,965	0	G	C	97.4%	134.2 / NA	39	E106D (GAG→GAC)	bglG	transcriptional antiterminator
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (19/19); minor base A (1/0); total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAT  >  CP000730/2274837‑2275096

cTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACaa                                                                                                                         >  2:396896/1‑141 (MQ=255)

                      cTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCTAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTg                                                                                                   >  1:351933/1‑141 (MQ=255)

                        gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc                                                                                                 <  1:350660/141‑1 (MQ=255)

                        gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc                                                                                                 <  1:104815/141‑1 (MQ=255)

                        gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc                                                                                                 >  2:86894/1‑141 (MQ=255)

                          tttAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGt                                                                                               <  2:218924/141‑1 (MQ=255)

                          tttAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCAGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAACGAACCAGTTAAACAATATAGTTTAACGCAAGCACTTGGCGt                                                                                               >  2:273468/1‑141 (MQ=255)

                             aaaCGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAACATAGTTTAGCGCAAGAAATTGGCGTTTc                                                                                            >  2:393967/1‑141 (MQ=255)

                                cGATTTGAAGCAATCGATTGCACAACATCAAACCATTGGCTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGt                                                                                         <  2:335751/141‑1 (MQ=255)

                                 gATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTTATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTc                                                                                        <  1:20562/141‑1 (MQ=255)

                                  ttgtGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGTAAGTAATTATTATATACGCTTTGAGTCAAGCCAAGGACCCAGTTAAACAATGTAGTCTATCGCAAGAAATTGGCGTTTCTGTcc                                                                                       >  2:100844/4‑141 (MQ=255)

                                  aTTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTcc                                                                                       <  1:372528/141‑1 (MQ=255)

                                  aTTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTcc                                                                                       <  1:298501/141‑1 (MQ=255)

                                     tGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGa                                                                                    >  2:369903/1‑141 (MQ=255)

                                      gAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATTTAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGAc                                                                                   <  2:155766/141‑1 (MQ=255)

                                       aaGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACt                                                                                  >  2:296483/1‑141 (MQ=255)

                                       aaGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACt                                                                                  >  1:170999/1‑141 (MQ=255)

                                         gCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACttt                                                                                <  1:369132/141‑1 (MQ=255)

                                                      caacaTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTa                                                                   <  2:247558/141‑1 (MQ=255)

                                                           tCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgatga                                                              <  1:403968/141‑1 (MQ=255)

                                                           tCAAACCATTGACTTATCTGTTAAAGAGCATAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgatga                                                              <  1:15029/141‑1 (MQ=255)

                                                                ccATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTag                                                         >  2:315135/1‑141 (MQ=255)

                                                                       cTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGa                                                  >  2:203363/1‑141 (MQ=255)

                                                                               ttGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATa                                          >  1:107199/1‑141 (MQ=255)

                                                                                    gagCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTAc                                     <  1:375881/141‑1 (MQ=255)

                                                                                      gCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCa                                   >  1:10077/1‑141 (MQ=255)

                                                                                        aGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAAc                                 <  2:46989/141‑1 (MQ=255)

                                                                                         gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt                                <  2:261751/141‑1 (MQ=255)

                                                                                         gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt                                <  2:47494/141‑1 (MQ=255)

                                                                                               aaTTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATCGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCttt                          <  2:184359/141‑1 (MQ=255)

                                                                                                attattATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTa                         >  1:287736/1‑141 (MQ=255)

                                                                                                      atataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGa                   >  1:403615/1‑141 (MQ=255)

                                                                                                      atataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGa                   >  1:189969/1‑141 (MQ=255)

                                                                                                      atataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGATTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTATCAACTATCTTTATCTCGa                   >  1:164375/1‑141 (MQ=255)

                                                                                                         taCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAg                >  1:232588/1‑141 (MQ=255)

                                                                                                               ttGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGc          <  1:263073/141‑1 (MQ=255)

                                                                                                                tGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCg         >  1:335546/1‑141 (MQ=255)

                                                                                                                     aaaGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGc    <  2:111375/140‑1 (MQ=255)

                                                                                                                       aGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAt  >  1:327566/1‑141 (MQ=255)

CTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAT  >  CP000730/2274837‑2275096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A