Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F109 I1 R1
|
27 |
23.1 |
511165 |
92.8% |
474361 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
2,184,430 |
G→T |
T176K (ACA→AAA) |
yhgF ← |
S1 domain RNA‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,184,430 | 0 | G | T | 100.0%
| 45.3
/ NA
| 16 | T176K (ACA→AAA) | yhgF | S1 domain RNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (9/7); total (9/7) |
AAACTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTGTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTG > CP000730/2184293‑2184565
|
aaaCTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTc < 2:115644/141‑1 (MQ=255)
cGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGaa < 2:19818/141‑1 (MQ=255)
cATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATACTATCTTGTGCAcc > 2:38672/1‑141 (MQ=255)
aTTTCACAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATCTCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGTAATAATAtttttttctcct > 2:113744/1‑133 (MQ=255)
tttCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGTAATTATTTCTTGTGTACttt > 2:58771/1‑141 (MQ=255)
aTACCTTTTTCATCTTCCGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCa < 2:146044/141‑1 (MQ=255)
ttCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCa > 2:464429/1‑141 (MQ=255)
aTGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAAtt < 2:210399/141‑1 (MQ=255)
tGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAAttg > 2:234902/1‑141 (MQ=255)
gTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAATttgt > 2:60650/1‑141 (MQ=255)
cacacCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAGTTTGTTCCGCAATAATATCTTGTGCACCTTTGGTAGCATCTTCAACCCATTGCACTTCTTCCCTTATAAATTGTTGTTCttt > 2:52631/1‑141 (MQ=255)
cTTGATGATACATATCTTTTAAAATTTTTTTTTTTTTTTTAGGATTATCTGAAATTTGTTCCGCAATCATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATACATTGTTGTGCTTTttctt > 2:59129/1‑141 (MQ=255)
atatatCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTgaga < 2:415650/139‑1 (MQ=255)
atCTTTTAAAATTTTTTTTCTATATTTAGGCTTATCTGAAATTTGTTCCGCAAAAATATCATGAACCCCATTGATATCACGTTCAACCGACTGCACATCATCATTCATAAAGTGTTGTTCTTTTTCCTCAATTGAGACTTc > 2:342468/1‑141 (MQ=255)
ttttAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATg < 2:300689/141‑1 (MQ=255)
aaaaTTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTa < 2:496217/141‑1 (MQ=255)
ttttttttCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGCTTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTg < 2:226469/141‑1 (MQ=255)
ttttttttCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTg < 2:368564/141‑1 (MQ=255)
|
AAACTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTGTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTG > CP000730/2184293‑2184565
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
