Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F31 I1 R1	9	193.0	4079480	90.0%	3671532	259.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,145,313	C→A	S683Y (TCC→TAC)	yegE →	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,145,313	0	C	A	97.6%	736.8 / NA	209	S683Y (TCC→TAC)	yegE	putative diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base C (3/2); new base A (103/99); total (106/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATG  >  NC_000913/2145044‑2145585

agaagCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                                                                                                                                                               <  2:531435/275‑1 (MQ=255)

  aagCGTTAGGTGTGCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatga                                                                                                                                                                                                                                                                              <  1:156346/274‑1 (MQ=255)

             gtTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACgac                                                                                                                                                                                                                                                                  >  2:984587/1‑273 (MQ=255)

                             tgtTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGCCGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGCGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGcc                                                                                                                                                                                                                                                 >  1:842167/1‑275 (MQ=255)

                             tgtTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCgcgc                                                                                                                                                                                                                                                  <  1:29768/274‑1 (MQ=255)

                                tGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGGTAAGTACTCCGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATTACGCAAAATGCTGCGCCAGCTGAGCTACCGAGCCTACCCGGATGCACTGACGC‑ATCCCGGCCACcgccgacgg                                                                                                                                                                                                                                              >  3:473432/1‑269 (MQ=255)

                                     atTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATTTCGCCAACCGCCCCAG‑TTTTgag                                                                                                                                                                                                                                       >  2:132874/1‑276 (MQ=255)

                                       tACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGAGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTgag                                                                                                                                                                                                                                       >  1:958438/1‑274 (MQ=255)

                                          ttttGGGGACAACGGACCATTAAGGGGGAACATTTACCGTGCCGACACCTCAC‑GTTCCGCGATCGGGCAAGATGGGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAaaca                                                                                                                                                                                                                                  <  4:88950/276‑1 (MQ=255)

                                           tttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAaaca                                                                                                                                                                                                                                  <  2:359403/275‑1 (MQ=255)

                                           gttGGCGAGAACCGAGAAGTAATGGATAACATGTAGCGCGCCGA‑GGCACACGGTTGCGCGATCGAGCAAGATGGGGTGATGCACGGTCGGAGCGGGGGCAGTTACGACGTGCATTACAGTATTACTCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGAACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAgacaa                                                                                                                                                                                                                                 <  4:50886/275‑1 (MQ=255)

                                            ttGGCGACAACGGACCATTAATTGAGGACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAaaca                                                                                                                                                                                                                                  <  1:651611/274‑1 (MQ=255)

                                            ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACTGCAGCAATATTGGTTTTGTTTTGGTGATTCATGACGTTACCGAATCACGCAAACTGCTTCGCTAGCTGTGCTAGTGCGCCTACCATTATTCCCTGTCGC‑CTCCCCCCCACCGTTCCAGCTTTTGATaacaa                                                                                                                                                                                                                                  >  4:89750/1‑272 (MQ=255)

                                            ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTTCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCCGGCCAACCGCGCCAG‑TTTTGAGaaa                                                                                                                                                                                                                                    >  1:127299/1‑272 (MQ=255)

                                                  aCAACGCACAAGTAATGGGGAACATTTACAGTGCCCACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAATTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTa                                                                                                                                                                                                                          <  2:348983/276‑1 (MQ=255)

                                                             ttAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                 <  1:521774/274‑1 (MQ=255)

                                                              tAATGGAGAACCTTTACAGTGCCGCAACCCCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCaa                                                                                                                                                                                                               <  4:269477/275‑1 (MQ=255)

                                                                 tGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGGTCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  3:48780/275‑1 (MQ=255)

                                                                      gaCATTTGCAGTGACGAAACATAAC‑GTTCCGCGATCGAGGATGGTGTGGTGTTGCACTGTCTGAGCGGCGGCAGTTACAGCGTGCATTACAGTATTACGCCGTTAAGTACTCGGGACGGCAGCAATATTGGTTCGGTACTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTGCCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAGCGGTaaa                                                                                                                                                                                                       <  2:55665/274‑1 (MQ=255)

                                                                        cATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTACGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCCAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACCACTGCGTATCCTGCTGCAAACGGTAACCa                                                                                                                                                                                                     >  2:306589/1‑275 (MQ=255)

                                                                        cATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATTTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAACCa                                                                                                                                                                                                     >  1:558245/1‑275 (MQ=255)

                                                                            gaCAGTGCAGACACCTCAC‑GGTCGGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  2:274141/273‑1 (MQ=255)

                                                                                gTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATATGGTGTTGCACTGTCGGAGCGGCCGCAGTTCCGACCTGCCTTACAGTATTACGCCGTTAAGTACACTGGACGGTAGCCATATTGGGTCGGTTCTGGTGTTTCATGACGTTACCGAATCACGCAAAATGCGGCGCCAGCTGAGCTACAACGGCTACCCTGACCCTTAGACAC‑CTCTCGCTTACCCAGCCAG‑TTTTGAGAAAAAAACGCGTTACCTGCTGAAAACGGGAAACCGTcca                                                                                                                                                                                                >  3:639669/1‑272 (MQ=255)

                                                                                 tGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCa                                                                                                                                                                                           >  2:945108/1‑276 (MQ=255)

                                                                                   ccGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGc                                                                                                                                                                                         >  2:410182/1‑276 (MQ=255)

                                                                                      gacccTCAC‑GTCCCTGGTTGGAGCTAGATGTGTTGTTGCCCTGTCGGGGCGGCGGCAGTTAAGACGTGCATTACAGTATTCAGCCGTTAAGTACTCTGGACGGCAGCAATATTCGTTCGGTTCGGGTGATTCAGGACGTTACCCAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  4:493802/273‑1 (MQ=255)

                                                                                       cacCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  3:8569/263‑1 (MQ=255)

                                                                                       cacCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  1:9414/275‑1 (MQ=255)

                                                                                       cacCTAAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  1:805449/275‑1 (MQ=255)

                                                                                           tCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGc                                                                                                                                                                                  >  1:329598/1‑275 (MQ=255)

                                                                                                gTTCCGCGATCGAGCAAGGTGTGGTGTTTGACTGTCGGAGCGGAGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTg                                                                                                                                                                              <  3:166448/275‑1 (MQ=255)

                                                                                                gTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCATCCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCt                                                                                                                                                                               <  3:426019/274‑1 (MQ=255)

                                                                                                    cgcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGgtgt                                                                                                                                                                          >  2:974075/1‑275 (MQ=255)

                                                                                                       taTCGAGCAAGATGGGGTGGTGCACTGTCGGAGCGGCGGCAGTTGCGACGTGCATTAGAGTATTACGCCGTTAAGTACTCGGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                      <  4:619468/275‑1 (MQ=255)

                                                                                                        aTCGAGAAAGAGGGGGTGGTGCCCGGTCAGAGCGGCGGCAGATACTACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                      <  3:891549/275‑1 (MQ=255)

                                                                                                            aGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAt                                                                                                                                                                  >  3:982712/1‑275 (MQ=255)

                                                                                                                 gaggtggtggtgCACGGTCGGGGCGGCGGCAGTTACGACGTGCATTACCGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGa                                                                                                                                                             <  4:974075/272‑1 (MQ=255)

                                                                                                                  aTGTGGGGTTGCACTGTCGGGGGGGCGGCAGTTACGTCGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCAAATCACGCAAAATGCTGCGCCCGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                                                                                                                                                            <  3:612771/275‑1 (MQ=255)

                                                                                                                      ggtgTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCCTGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGCAACAACTGCGTATCCTGCTGCCAACGGTAACCCGTACACATCAGCGCCATGCCCTGGTGTTTATCGATCTGTATCGCt                                                                                                                                                        >  1:727289/1‑275 (MQ=255)

                                                                                                                       gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTTACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACCTCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCttt                                                                                                                                                      >  2:568104/1‑276 (MQ=255)

                                                                                                                            gCACTGGCGGAGCGGCGGCAGTTGAGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGA‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  4:699368/275‑1 (MQ=255)

                                                                                                                             cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  1:99456/274‑1 (MQ=255)

                                                                                                                             cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑CTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAga                                                                                                                                                 >  2:104374/1‑274 (MQ=255)

                                                                                                                             cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATCATGCACTGACGC‑ATCTCGCCCACCGCGCCAG‑TTTTGAGAAACAACTGCGTCTCCTGCTGCAAACGTTAAACAGACCACATCAGCAACATGCCCTGGTGTTTGTCGATCTTGTACGCGTTAAAGc                                                                                                                                                 >  4:746494/1‑275 (MQ=255)

                                                                                                                             cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATCACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCg                                                                                                                                                <  2:827387/276‑1 (MQ=255)

                                                                                                                                  tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAATATGCTGCGCCAGCTGAGCTACAGTGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCCG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGCTCTTGATCGCTTTAAAGCGGTg                                                                                                                                             >  1:790656/1‑274 (MQ=255)

                                                                                                                                       gcggcgGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTg                                                                                                                                                                              <  4:835540/236‑1 (MQ=255)

                                                                                                                                          gcggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTANCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCCCTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACACCTGCGTATCCTGCTGCAAACGGTAAACAGTACCAAACCGCGACATGCCCTGGTGTTTATCGATCTGTATCGCTTTAAAGCGGGGACTggccgcc                                                                                                                                   >  4:545589/1‑270 (MQ=255)

                                                                                                                                          gcggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCCCTGACGC‑ATCTCGCCAACCGCGCCCG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTACACAGTACCCATTACCGACATTCCCCGGTGTTTATTGATCTTGATCGCTTTAAAGCGGTGAATGACAccg                                                                                                                                   >  4:878727/1‑276 (MQ=255)

                                                                                                                                                    cgacgTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATTCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCAt                                                                                                                           <  1:140130/274‑1 (MQ=255)

                                                                                                                                                         tGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCCG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATgaggc                                                                                                                      >  2:636812/1‑274 (MQ=255)

                                                                                                                                                         tGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACAATGATGCACTGAAGC‑ATCTCGCCAACCGAGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTACAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGACCTTTATCGTTTTAAACCTGTGAATTAAACCGCCgggacgtcggcgt                                                                                                                    >  4:433620/1‑266 (MQ=255)

                                                                                                                                                           cATTACGGTATTAAGCCGTTAACTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCTCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                   <  2:842348/275‑1 (MQ=255)

                                                                                                                                                           aaTTACAGTATTAGGCCGTTAAGTACTCGAGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                   <  2:685242/274‑1 (MQ=255)

                                                                                                                                                                     ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGGGAATGACAGCGCCGGGCATGCGGCGGGTGCCGTTTtgc                                                                                                        >  2:720754/1‑276 (MQ=255)

                                                                                                                                                                     ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTAAAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACACGCCCTGGTGTTTATCGACCTTGACCGCTTTAAAGCGGTGTATGACAGCCCCGGCCATGCGGCGGTTGACTCGTtg                                                                                                         >  2:454648/1‑275 (MQ=255)

                                                                                                                                                                        cGCCGTTAGGTACTCTGGACGGCAGAAATATTGGTTCGGTTCTGGTGATTCAGTACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgct                                                                                                       <  1:361491/274‑1 (MQ=255)

                                                                                                                                                                        cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCg                                                                                                                                                                    <  2:506626/213‑1 (MQ=255)

                                                                                                                                                                        cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGAGAATGACAGCGCCGGGCATCTGGCGGGTGGCCCTTtgctg                                                                                                      >  1:262080/1‑275 (MQ=255)

                                                                                                                                                                         gCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  3:935285/182‑1 (MQ=255)

                                                                                                                                                                             ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa                                                                                                 >  1:178126/1‑275 (MQ=255)

                                                                                                                                                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCCAACGGTAAACAGTACAAATCAGCGACATTCCCTGGTGTTTAGAGATCTTGATCGCTTTa                                                                                                                                                     >  4:946000/1‑213 (MQ=255)

                                                                                                                                                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTacac                                                                                                                                                                                               >  2:380150/1‑171 (MQ=255)

                                                                                                                                                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACCTGCCCGGGTGTTTATCGATCTTGATCGCTTTAACGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGTGTCGCGAACCTGcgccgt                                                                                      >  4:44768/1‑276 (MQ=255)

                                                                                                                                                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATg                                                                                                                                                                                   >  3:537947/1‑183 (MQ=255)

                                                                                                                                                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtcgt                                                                                      >  4:126030/1‑276 (MQ=255)

                                                                                                                                                                                       tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTCTATCGATCtt                                                                                                                                                               >  2:889094/1‑203 (MQ=255)

                                                                                                                                                                                         gACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  3:682164/178‑1 (MQ=255)

                                                                                                                                                                                          aCGGCAGCAATATTTGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATc                                                                                                                                                                                            <  4:380150/171‑1 (MQ=255)

                                                                                                                                                                                           cGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCt                                                                                                                                                                               <  1:537949/183‑1 (MQ=255)

                                                                                                                                                                                                 caATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt                                                                               <  1:925906/273‑1 (MQ=255)

                                                                                                                                                                                                                    gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGACCTTGATCGCTTTAAAGCGGTGAAGGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTTCGCGAACTGGCGTCGTTTATGCCGGGTTTGGTGCGTTCCAGCg                                                           >  3:254992/1‑274 (MQ=255)

                                                                                                                                                                                                                       aTTCAGGACGTGCCCGAACAACGCCACATGCTGCGCCAGCGTAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCCTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTg                                                      <  4:209125/276‑1 (MQ=255)

                                                                                                                                                                                                                         tCAGGACGTTACGTAATCACGCAAAATGCTGCTCCAGCTGCGGTACACCGCCTACCTAGATGCACTGACGC‑GTTTCGGTAACCGCGACAG‑TTTTGAGAAACAACTGCGTATCCGGCTGCAAACGGTGAACAGTACAAATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTGAAAGCGGTGAATGACAGCGCCGGGAATGCGGGGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCAAGCGACGTg                                                      <  3:558243/274‑1 (MQ=255)

                                                                                                                                                                                                                         tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTAACCTGCTGCAAAAGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGCGACGCTTTGCTGCGCGCACTGGCGTCGTTAATGCCAAGTGTGCTTCGTTCCAGCGACGTgt                                                     >  1:389108/1‑274 (MQ=255)

                                                                                                                                                                                                                                gTTACCGAAGCCCGCAAAATGCCGCGCCAGCTGAGCACCACCGCCTACCATGAGGCACTGACGA‑AGCTCGCCAACCGCGCCAG‑TGTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCGTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCATCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGa                                             <  4:324596/276‑1 (MQ=255)

                                                                                                                                                                                                                                 ttACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAg                                                                                                                                                                                                    >  1:577089/1‑124 (MQ=255)

                                                                                                                                                                                                                                 ttACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATTACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGATTATGCTGCGTTCCCCCGACGTTCCTGcgcgg                                             >  3:225651/1‑274 (MQ=255)

                                                                                                                                                                                                                                  tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGGCGTTAAAGCTGAGTATGCGGAGTTTCAGGCACGTGCTTGCGCGACt                                           >  4:926093/1‑276 (MQ=255)

                                                                                                                                                                                                                                   aCCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCTGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTc                                          >  2:206951/1‑276 (MQ=255)

                                                                                                                                                                                                                                      gAATCACCCAAAATGCCTCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCgg                                        <  3:831646/275‑1 (MQ=255)

                                                                                                                                                                                                                                       aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAaacaac                                                                                                                                                                                                                                >  1:454398/1‑90 (MQ=255)

                                                                                                                                                                                                                                       aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGt                                       >  3:79729/1‑275 (MQ=255)

                                                                                                                                                                                                                                         tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGAGTTTATCGAGCTTGATCGCTTTAAAGGGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGCACAGGCGTCGGTAATGCCGAGTATGGTGCGTTTCAGCGCTGTGCTGTGGCGCGTTGGTggg                                    >  2:774448/1‑276 (MQ=255)

                                                                                                                                                                                                                                            cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGGTGAGTTTGCTTCGTTCCAGCGGCGTTCTGGGGCGAcc                                           >  3:4300/1‑265 (MQ=255)

                                                                                                                                                                                                                                            cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTggg                                    >  1:761155/1‑273 (MQ=255)

                                                                                                                                                                                                                                                aaaGGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGt                                       <  1:4300/266‑1 (MQ=255)

                                                                                                                                                                                                                                                 aaTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGTTCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAAGGCTGAGTATGCTGCGTTCCAGCGACGTGCGGGCGCGACCCGGGGGGGCTGag                              >  1:206887/1‑273 (MQ=255)

                                                                                                                                                                                                                                                     ctgcGCCAGCTGGACTACATCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt                        <  4:1006983/276‑1 (MQ=255)

                                                                                                                                                                                                                                                      tgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCCGCGACGTGCTGGCCCGACTCGGTGGGGTTGATTTTgg                         >  3:110003/1‑274 (MQ=255)

                                                                                                                                                                                                                                                      ggcgcCAACTTAGCTCCCTCGCCTACCATAATGGCCGAACGC‑AGTTCGCCAACCGCGCCAG‑TGTTGCGAAACAACTGCGTATCCTGCTGCAACCGGTAAACAGTACACACCAGCGACATGCCCTGGTGTGTATCTATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt                        <  3:288250/274‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcgaGCTGAGCTACCGCCCCGACCATGATGC‑CTTACGCAAGCTCCACAACCCCGCCAG‑TTTTGAGAATCAACTGCTTATCCTGCTGCAAACGGTAACCAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  4:161626/273‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  2:1018420/276‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  2:212673/276‑1 (MQ=255)

                                                                                                                                                                                                                                                          ccAGCTGAGCGTCAGCCCCTACCATGATTAACTGACAC‑ATCTCGCCCACCGCGCCAG‑TTTTGATAAACAACTGCGTATCCTGCTGCAAACAGTAAACAGTACACATCGGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  3:127298/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                  gCTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa            <  1:70035/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                   cTACAGCGCCTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCTTGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa            <  1:982714/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                           ccTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGAGTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAt   >  1:963387/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                           ccTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGTGGGTGAATTTGGTTTGCTGCTACCCGACTGTAATg  >  2:786435/1‑276 (MQ=255)

                                                                                                                                                                                                                                                                           ccTACCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAAATGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTCCTGGCGCGACTCGGGGGGGCTGAATTTGGTCTTCGGCTACCGGTCTGTGATg  >  4:733355/1‑276 (MQ=255)

AGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCAC‑GTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGC‑ATCTCGCCAACCGCGCCAG‑TTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATG  >  NC_000913/2145044‑2145585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A