Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F44 I1 R1 10 113.9 2297240 89.1% 2046840 259.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA99.2% 429.6 / NA 122S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (75/46);  minor base T (0/1);  total (75/47)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145040‑2145584
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
aGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCat                                                                                                                                                                                                                                                                               >  2:152036/1‑278 (MQ=255)
              ggTGTGCCGATCCTGACGGTGTTGCATATGAATGTGGGAGACAACGGACAATTAATGGAGGACATGTACAGTGCCGACACCTCACGTTCCGAGCTCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGc                                                                                                                                                                                                                                                                  <  2:16084/277‑1 (MQ=255)
                  ttCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑t                                                                                                                                                                                                                                                               <  1:136297/275‑1 (MQ=255)
                     cGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGtt                                                                                                                                                                                                                                            >  3:302307/1‑275 (MQ=255)
                      gCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCGGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCACTATTTGTTCGGTTCTGGGGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGCGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCa                                                                                                                                                                                                                                                        >  4:513900/1‑278 (MQ=255)
                                gtgttttgaatTACTTTTAACGGAAAAGGAACGTGAGTGGAGAACATTTACAGTGGCGCCACCAAACGTTCCGCGTTCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTGCGACGTGGATTACAGTATTACGCCGATCAGTACTGTGGACGGCATCAATATTTGTTCGGTTCTGGTGATTCAGGTCGTTACCGTATCACGCAAAATGCTGCGCTCGCTGAGGTACAGCGCCTACCATGAGGCACTGACGTA‑TCTCGCCAACCgcgc                                                                                                                                                                                                                                                 <  3:556230/266‑1 (MQ=255)
                                 tgtTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACACTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAAGATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCCACAGCCGAGCTACAGCGCCTACCATGACGACATGACGCA‑GCTCGACCACCGCGCCCGt                                                                                                                                                                                                                                             >  4:158033/1‑278 (MQ=255)
                                            aCTTTTGGCGACAACGGACCATTAATGGAGAACATTTAAAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTgaga                                                                                                                                                                                                                                      <  1:604554/274‑1 (MQ=255)
                                                  ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACt                                                                                                                                                                                                                               >  1:123838/1‑275 (MQ=255)
                                                  ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCg                                                                                                                                                                                                                            >  2:83956/1‑278 (MQ=255)
                                                     gACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                                                                                                                                                             <  1:158004/274‑1 (MQ=255)
                                                       cAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTa                                                                                                                                                                                                                          <  1:72318/275‑1 (MQ=255)
                                                                aatAATGGGGAACATTTGCAGTCCCGAGACCTCCCGTTCCGCGTTCGAGAAAGATGTGGGGTTGCACGGTCGGGGGGGCGGCAGGTACGACGTGCATTACAGTCTTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTTGTGCTTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACGGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                 <  3:528137/273‑1 (MQ=255)
                                                                  taaggggggaCAGTTACCGTGCTGAACCCTGACGTTCCGAGATGGATCATGATGTGGTGTTGCACTGGCGGAACGGCGGCAGTTACGGCGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  4:476418/269‑1 (MQ=255)
                                                                     tGGCGAACATTGACAGGCCCGACAACCCACGTCGCGCGAGCGAGCAAGGTGTGGTGTTGCACGGTCGGAGCGGCGGCAGTTACTACGTGCAGTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGTTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  3:248766/275‑1 (MQ=255)
                                                                        ggaAAATCTACTGTGCCGACACCTCCCGTTCCGCGGTCGAGGAAGATGGGGTGTTGCCCGGTCGGAGCGGCGGCAGTTACGAAGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGAAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       <  4:152036/276‑1 (MQ=255)
                                                                        agaACATTTACAGGGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       <  2:298009/277‑1 (MQ=255)
                                                                          aaCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGCCGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       >  1:593812/1‑275 (MQ=255)
                                                                          aaCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       >  1:99195/1‑275 (MQ=255)
                                                                            cATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  1:302307/274‑1 (MQ=255)
                                                                            cATTCACATTGCCGGCGCCCAGCGTTCCGCGACAGAGCAAGATGTGGTGTGGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  2:4005/278‑1 (MQ=255)
                                                                               ttACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  1:197223/275‑1 (MQ=255)
                                                                                 aCAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCCCCAGTTTTGAGAAACAACTGCGTAACCTGCTGCAAACGGTAAACATTac                                                                                                                                                                                                 >  1:277599/1‑274 (MQ=255)
                                                                                    gTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTaca                                                                                                                                                                                                >  1:409583/1‑272 (MQ=255)
                                                                                    gTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGGTTTGAGAAACAAATGCGTATCCTGCTGCAAACGGTAAACAGTaca                                                                                                                                                                                                >  1:245721/1‑272 (MQ=255)
                                                                                       ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGACACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGa                                                                                                                                                                                       >  2:380887/1‑278 (MQ=255)
                                                                                        cGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGCTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  2:488110/278‑1 (MQ=255)
                                                                                             ccTCCCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                                 <  2:96242/278‑1 (MQ=255)
                                                                                               tCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAACCAGTACACATCAGCGACATcccc                                                                                                                                                                                >  2:118580/1‑277 (MQ=255)
                                                                                                cACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                                 >  1:586621/1‑275 (MQ=255)
                                                                                                    ttCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                                 <  1:50636/271‑1 (MQ=255)
                                                                                                       cgcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATAATGCGCCAGCTGAGCTACAGGGCCTACCATTATGCACTGACGCA‑TCCATCAAACCGCCCCAGCTTTGAGAAACAAATGGGCATCCTGCTGCAAAAGGT‑AACAGTACAAAGCAGAGACATGCACAGGTGtttt                                                                                                                                                                       >  2:366024/1‑276 (MQ=255)
                                                                                                        gcgATCGGGGAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                      <  2:271947/278‑1 (MQ=255)
                                                                                                        gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                      <  2:47385/278‑1 (MQ=255)
                                                                                                                   agagGTGGTGGTGGACGGTCGGGGCGGCGGAAGTTACGAAGTGCATGACAGGATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTGCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                                                                                                                                                            <  4:445121/277‑1 (MQ=255)
                                                                                                                          gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATcc                                                                                                                                                                                                                       >  2:146222/1‑211 (MQ=255)
                                                                                                                           tgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTTAGAAACAACTGCGTATCCTGCTGCAAACGGGAAACAGTACTCATCAGCTGCATGCCCTGGTGTTTAGTGATCGTTATCGCttt                                                                                                                                                      >  3:76150/1‑275 (MQ=255)
                                                                                                                             ttGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCCCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTAACCTGCTGAACAATGTAAACAGTCAACATCAGCGACATCCCCTGGTGTTTATCGAACTTGCACCCTTTaa                                                                                                                                                    >  3:493230/1‑275 (MQ=255)
                                                                                                                             ttGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTATGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGAACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  2:191180/277‑1 (MQ=255)
                                                                                                                              tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCAAGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCC‑TCTCGCCAACCGCGCCAGTCTTTAGAACCAACTGCGTATCCTGCTTCAAACGGTAAACAGTACACATCAGCGACATGCCCTGATGTTTATCGTTCTTGATCGCTTTat                                                                                                                                                    >  1:556908/1‑273 (MQ=255)
                                                                                                                                cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTTTTTATCGATCTTGATCGCTTTAAAGc                                                                                                                                                 >  1:45745/1‑275 (MQ=255)
                                                                                                                                cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCgg                                                                                                                                               >  2:494712/1‑277 (MQ=255)
                                                                                                                                agcggttggAGCGGCGGCAGTTACGACGTGCATTACAGTAGTACGCCGGTAAGCACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  1:132275/267‑1 (MQ=255)
                                                                                                                                     tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACcgcg                                                                                                                                                                                                                                                  >  4:465673/1‑173 (MQ=255)
                                                                                                                                      cGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCgcgc                                                                                                                                                                                                                                                 <  2:465673/173‑1 (MQ=255)
                                                                                                                                          gcggagGCACTTACGACGTGCATTACAGTATTACGCCGATAAGTACTCTGGACGGCCGCAATTGTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCGGCTGAGCTACTGCGCCTACCATGAGGCACTTACGCA‑TCTCGCCACCCGCACAAGTTTTGAGAAACAACTGCGTAGCCTGCCGCAGACGGACCACATTTCACATCACCGACATGCCCTGGTTTTTACCGGTCTTGTTCGCTTTAAAGCGGTaaactac                                                                                                                                       >  3:607933/1‑271 (MQ=255)
                                                                                                                                               ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTTATCGCTTTAAACCGGTGAATGAGTGCGCCgg                                                                                                                               >  2:438582/1‑278 (MQ=255)
                                                                                                                                               ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TATCGCGAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGAGACATGCACTGGTGTGTATCGACCTTGATCGCTTTAAAGCGGTGAATGAAAgcgc                                                                                                                                  >  1:418193/1‑275 (MQ=255)
                                                                                                                                                              cATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                   >  1:442097/1‑275 (MQ=255)
                                                                                                                                                              aattcaaGTATTACGCCGTGAAGTACGCTGGCCGGCAGCAAGATTGGTTCGGTTCTGAGGATTCAGGACGTGACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                   <  3:161319/269‑1 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATCAGCGACATGCCCTGGTGTTTCTCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGACGGGCCTGCGGCGGGTGAGGCTTtg                                                                                                         >  3:327608/1‑275 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGATATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCCTGCGGCGGGTGACGCTTtgc‑tg                                                                                                     >  2:109923/1‑278 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgc‑tg                                                                                                     >  2:468110/1‑278 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtg                                                                                                         >  1:40459/1‑275 (MQ=255)
                                                                                                                                                                          aCGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTg                                                                                                                                                                                                                                         <  1:57876/145‑1 (MQ=255)
                                                                                                                                                                           cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAACCGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGGAGCGCTATGC‑Tgcgc                                                                                                  >  4:473526/1‑278 (MQ=255)
                                                                                                                                                                           cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgc‑tg                                                                                                     >  3:353157/1‑275 (MQ=255)
                                                                                                                                                                              cGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGac                                                                                               >  2:446454/1‑277 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCat                                                                                                                                                                                                                                                                               >  4:406388/1‑102 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGa                                                                                                >  1:332694/1‑275 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGa                                                                                                >  1:108413/1‑275 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTCGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGTCCTGGTGTTTATCGATCTTGATCGCTTTAAAGAGGTGAATGACAGCGCCGGGACTGCGGAGGGTGACGCTTtgc‑tggcc                                                                                                  >  3:33826/1‑270 (MQ=255)
                                                                                                                                                                                 tAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                                                                                                                                                              <  2:406388/102‑1 (MQ=255)
                                                                                                                                                                                        tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACTGCTTGT‑TTCGCGAACTGGcgtcgt                                                                                     >  4:514060/1‑278 (MQ=255)
                                                                                                                                                                                        tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTAACCTGCTGCAAACGGTAAACAGTACACATCAGCGACATCCCCTGGTGTTTATCGATCATGACCGCTTTAAAGCCGTTAATGAAAGCGCCGGGAATGCCGCGGGTGACGCATTGCGTGC‑CGAACTGGccgcgt                                                                                     >  4:135804/1‑273 (MQ=255)
                                                                                                                                                                                          tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAAc                                                                                                                                                                                                             >  4:442690/1‑157 (MQ=255)
                                                                                                                                                                                          tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGcgtcg                                                                                      >  1:549688/1‑275 (MQ=255)
                                                                                                                                                                                          tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGGGACGCTTTTC‑TGCGCGAACTGGccgtg                                                                                      >  3:223186/1‑271 (MQ=255)
                                                                                                                                                                                           ggACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  2:442690/157‑1 (MQ=255)
                                                                                                                                                                                               ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATTTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGAGGGTGACGCGTGTC‑TGCGCGAACTGTcggctgt                                                                                    >  3:106372/1‑267 (MQ=255)
                                                                                                                                                                                                 cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGCTCGCTTTAAAGCGGCGAATGAAAGCGCCGGGCATGCGCCGGGTGCCGCCTCGC‑TGCGCGAACTGGCCTCGTTAATCCTGa                                                                            >  2:254975/1‑278 (MQ=255)
                                                                                                                                                                                                 cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAACTTGATCGCGTTAAAGCGGTGAATGACAGCGCCGGGCATGCGCCGGGTGAGGCTTTGC‑TGCGCGAACTGGCGGCGTTAATGcgtg                                                                            >  4:74760/1‑275 (MQ=255)
                                                                                                                                                                                                              tcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAACTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGACTCGGCGTCGGTAATGCTGAGTAtgcgt                                                                    >  3:202547/1‑271 (MQ=255)
                                                                                                                                                                                                                 gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCTCTTTAAAGCGGTGAATGCCAGCGACGGGCCTGCGGCGGGTCACGCTTTGC‑TGCCCTCACTTGCGTCTGTACTGCCGAGTTtgcttggtt                                                                >  4:494403/1‑269 (MQ=255)
                                                                                                                                                                                                                 gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCa                                                             >  2:44094/1‑277 (MQ=255)
                                                                                                                                                                                                                 gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAACGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTTTGGTGCGttgtag                                                            >  4:205481/1‑274 (MQ=255)
                                                                                                                                                                                                                       gTGATTGAGGACGATACACAAGAACGCAAAATGCCGGGCCAGCTGGGCAACCGAGCCTTACGTGATGCACT‑ACGGACTCTCGCCACCCGCGCCAGTTTTGAGAAAGAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAGATCAGCGACATACCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTc                                                               <  3:561304/269‑1 (MQ=255)
                                                                                                                                                                                                                       gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAAATCAGCGACATGCCCTGGTGTTTACCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCGGAGTATGCTGCGTTGCGTCGACGt                                                      >  4:130328/1‑278 (MQ=255)
                                                                                                                                                                                                                       gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTGTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGGAGGAGGCGTTCCAggg                                                          >  1:167511/1‑272 (MQ=255)
                                                                                                                                                                                                                           ttCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGccc                                                                                                                                                                                <  1:325757/153‑1 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCGGCGACATGCCCTGGTGTTTACCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTCGC‑TGCGCGTACTGGCTTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTgg                                                 >  2:425503/1‑278 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGGGCGTGACGCTTTTC‑TGCGCGCACTGGGTTCTTTACTGCTGCGTATTCTTCGTTTCAGCGACTTGc                                                    >  3:322554/1‑275 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCGGCGTTCAAGCGACGTGCTgg                                                 >  2:400533/1‑278 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACAGGCCCTGGTGTTTATACAGCTTCATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTCC‑TGCGCTAACTGGCGTCGTTCATGCTGATCATGCTGCGTTCCAGCGACGTGc                                                    >  3:207645/1‑275 (MQ=255)
                                                                                                                                                                                                                                gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGaa                                                                                               <  3:78924/228‑1 (MQ=255)
                                                                                                                                                                                                                                gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCCTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGgc                                                <  3:586621/275‑1 (MQ=255)
                                                                                                                                                                                                                                    ttACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACCGCGCCGGGCATGCGGAGGGTGCCGTTTTGC‑TGCGCGAAATGGCCGCGGTACTGCTGAGAATGCTGGGTGCCAGCCGCGTGCTGGggcggcct                                         >  4:461873/1‑274 (MQ=255)
                                                                                                                                                                                                                                      atcGAATCACGCAAAATGCGGCGACAGCCGAGCGACAGAGCCGACCATGATGCAAGGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAGGCTTTGC‑TGCGCGAACGGGCGTCGCTAATGCAGAGTATGCGGCGCTCCACGGACGTGGGGGGGCGAcc                                          >  3:388363/3‑274 (MQ=255)
                                                                                                                                                                                                                                         gAATCACGCAAGCTGCTGCGCAGGCAGGACCACACAGCATACCAGGATGCACGTACGCA‑GCTCACCAACCGCGCCAGTTTGGAAAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAGAGCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGGGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCgg                                       <  3:549688/275‑1 (MQ=255)
                                                                                                                                                                                                                                          aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCTGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTTCGTTCCCGCGACGTGCTTGCGCGACTCGGTggg                                   >  2:133393/1‑278 (MQ=255)
                                                                                                                                                                                                                                            tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGcgtc                                                                                       >  3:186541/1‑224 (MQ=255)
                                                                                                                                                                                                                                             cACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGATAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                 <  2:101957/277‑1 (MQ=255)
                                                                                                                                                                                                                                                gCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTa                                                                                   <  1:186541/224‑1 (MQ=255)
                                                                                                                                                                                                                                                             ccAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  1:322554/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             ccAGCTGAGCTAAAGCGCCTAACAGGATGCACTGACGCA‑TCTAGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  1:223186/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tagcataaaGCGCCGACAATGATGAACTGACGAA‑TCACGCCAACCGCGCCAGTTTTGAGAACCAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTa              <  3:123838/267‑1 (MQ=255)
                                                                                                                                                                                                                                                                    accTACAGGACCTACCAGTATGCACTGACGAA‑GCTCGCCAACCGGGCCAGTTTTGAGAAACAACGGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa         <  4:369632/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTACCGCGCCTAGCATGTGTCACGGACGCA‑GCGCGCCAACCGCGGCAGTTTTGAGGAGCAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGAc        <  4:482492/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tACAGCGCCTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa         <  3:108413/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccTACCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTACTGCTGAGTATGCTGCGTTCCAGCGACGTTCTGGCGCGagc                                          >  3:283882/1‑233 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
AGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCA‑TCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGC‑TGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145040‑2145584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A