Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F34 I1 R1 9 149.1 2973750 91.7% 2726928 262.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA97.9% 458.5 / NA 140S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (65/72);  total (66/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 140 total aligned reads displayed.

GAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGT  >  NC_000913/2145045‑2145581
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                               
gaagCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACTGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATAGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                                                                                                                                                            <  3:36444/274‑1 (MQ=255)
          gtgtTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAt                                                                                                                                                                                                                                                              <  2:125814/278‑1 (MQ=255)
             atcaggtCCTGACGGTGTTGCATATTACTTTTGGCGGCAACGGACCATTAATGGGGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTTGTGTTGCACTGTCGGAGCGGCGGCAATTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAt                                                                                                                                                                                                                                                              <  3:49136/269‑1 (MQ=255)
              tCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGCCAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGAATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGCTTCAGGCCGTTACCGCATCACGACAAATGCTGCGCCAGCCGAGTTACAGCGCCAAGCATGATGCAAGGACGcct                                                                                                                                                                                                                                                              >  3:748853/1‑272 (MQ=255)
                       gACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCCCCAGCTGAGCTACAGCGCCTACCATGATGCACTTACGCATCTCGCCAAcc                                                                                                                                                                                                                                                    >  1:229355/1‑275 (MQ=255)
                         cGGTTTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑gcgc                                                                                                                                                                                                                                               <  2:186583/277‑1 (MQ=255)
                            tgtTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGGTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGCTGCACTTACGCAGCTCGCCAACC‑GCGcc                                                                                                                                                                                                                                              >  3:533669/1‑275 (MQ=255)
                              ttGCATATTACTGTTGGCAGCAACGGACAATTGATGGAGAACCTTTACAGTGCCGACA‑CCTCCCCTTCCGCGATCGAGCAAGATGTGGGCTTGCACTGTCTGAGCGGCGGCAGTTACGACTTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACTTTAGCGAAGCACGCAAAAGGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑Gttt                                                                                                                                                                                                                                        <  2:50889/278‑1 (MQ=255)
                                      tACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGGCCACC‑GCGCCA‑GTTTTgcaa                                                                                                                                                                                                                                   >  1:316867/1‑272 (MQ=255)
                                      tACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCAAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCCCGCCAACC‑GCCCCA‑GTTTGgag                                                                                                                                                                                                                                    >  3:357097/1‑274 (MQ=255)
                                       aCTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTgaga                                                                                                                                                                                                                                   <  1:524899/274‑1 (MQ=255)
                                               tagcAACGGACCATTAATAGAGTAGCTTTACAGTGTCGAAA‑TGTAACGTGCCGGGATGGGGCAAGATGTGGTGTGGCACTGTCGGAGCGGCAGCAGTTACTACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGTTTCGGTTCTGGTGATTCAGGACGTTACCGAAACACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTa                                                                                                                                                                                                                       <  4:67206/275‑1 (MQ=255)
                                                 aCAACGGACCATTAATGGAGAAAATGTACGATGCCGACA‑CCTCACGTTCCGCGGTCGAGCAAGGTGTGGTGTTGCACTGTCGGAGAGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACGCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATc                                                                                                                                                                                                                     <  2:79004/278‑1 (MQ=255)
                                                     cGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGTGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTTCTTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGATGAGCTAACGCGCCTACCATTATTCCCTGACGCATC‑CCCCAACCAGCGCCA‑ATTGTGGTAaaaaacctgctgacctgc                                                                                                                                                                                                                 >  4:220223/1‑266 (MQ=255)
                                                     cGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCTTTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTCCAGCGCCTACCATCATGACCTGACGCATCTCGCCAACC‑GCGCCA‑ATTTTGAGAAACAACTGCCTATCctg                                                                                                                                                                                                                  >  4:270556/1‑277 (MQ=255)
                                                             tAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGNGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAAGATTGGTTCGGTTCTGGTGATTCAGTACGTTACCGAATCACGCAAAAAGAGGCGCCAGCTGAGCTACAGCGCCTACCATGATTCACTGACGAATTTCGCCAACC‑GCCCCA‑GTTTTGAGAAAAAAATGAGTATCcttatccaaaag                                                                                                                                                                                                         >  4:317201/1‑268 (MQ=255)
                                                              agagacagaCATTTACAGTGCCGATA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCTGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                         <  2:347042/269‑1 (MQ=255)
                                                              aaTGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCaa                                                                                                                                                                                                            <  1:600664/274‑1 (MQ=255)
                                                                gagaaagcATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                         <  1:812521/268‑1 (MQ=255)
                                                                  gagaACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGt                                                                                                                                                                                                       >  1:468075/1‑275 (MQ=255)
                                                                   agaACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                    <  2:66177/277‑1 (MQ=255)
                                                                   agaACATTTACAGTGCCGACA‑CCGCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                    <  2:529060/277‑1 (MQ=255)
                                                                   agaACATTGACAGTGCCGGCC‑CCTAACGTTCCGCGATCGGGCAAGATGTGGGTTTGCACTGTCCGGGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACGCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                    <  4:36362/277‑1 (MQ=255)
                                                                   agaACAGTTACATTG‑CGACACCCTCACTTGCCGCGTCCGAGGAAGGTGTGGTGTTGAACTGTGGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTCCGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                    <  4:297198/277‑1 (MQ=255)
                                                                   agaAAATTTACAGTGCCGAAA‑CCTCACGTTCCGCGATCGAGCAAGAGGTGGTGTTGGACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                    <  2:619648/277‑1 (MQ=255)
                                                                      aCATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                    <  3:530743/274‑1 (MQ=255)
                                                                       cATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCCGCAAACGGTCAAc                                                                                                                                                                                                   >  1:154256/1‑274 (MQ=255)
                                                                          ttccAGTGGCGACA‑GCTCACTTTCAGAGATCGGGCAAGGAGTGGTGTTGCGCTGTCGGATGGGCGGCAGTTACGACGTTCAGTAGAGTAGTACGGCGTTAAGTACTCTCGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGGGCCAGCTGAGCTACAGCGCCTACCAGGATGCACTGACGCATCTCGCCACCC‑GGGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                               <  3:809735/272‑1 (MQ=255)
                                                                          ttACAGTGCCGACC‑CCTCACGTTCGGCGATCGAACAAGATGTGGTGTTGCAGTGTCGGGGCGGGGGCGGTTACGACGTGCATTACAGTATTAAGCCGTTAAGTACGCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGAGTAAGGACGTTACCGAAGCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                               <  3:643156/275‑1 (MQ=255)
                                                                               gTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCGAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTCTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAACCAGTACACAt                                                                                                                                                                                          >  2:278851/1‑275 (MQ=255)
                                                                               gTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCAGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATCCAGGACGTTACAGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑CAGCCA‑GTTTTGAGAAACAACTGCGTATTCAGCGGCAAAAGGTACACAGTAaacaa                                                                                                                                                                                          >  2:599308/1‑274 (MQ=255)
                                                                                 gCCGACA‑CTCGACTTGCCGTGATCGAGGAAGATGGGGTGTTGCACTGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCGGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCg                                                                                                                                                                                     <  4:89114/278‑1 (MQ=255)
                                                                                 gCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCCCCA‑GTTTTGAGAAACAACTGACTACACTGCTACAAACGGTAAACAGTACACATCAgtg                                                                                                                                                                                     >  4:461987/1‑276 (MQ=255)
                                                                                 gCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGNTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAAGCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGAAGCATCTCGCCAACC‑GCCCCA‑GTTTTACGACACAACTGCGTATCCTGCTGCAAACGGTAAACaacaccggtcgctt                                                                                                                                                                                     >  4:347042/1‑265 (MQ=255)
                                                                                  ccGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGGGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGCGCTACAGCGCCTACCATGATGCACGGACGCATCTCGCAAACC‑GCGCCC‑GTTTTGAGAAACAACTGCGTATCCTGAGGCAAACGGTAAACAGTAAACATAAGCGa                                                                                                                                                                                    >  2:515030/1‑278 (MQ=255)
                                                                                  ccGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAg                                                                                                                                                                                       >  1:401842/1‑275 (MQ=255)
                                                                                  ccGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCCCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCCCCAACC‑GCGCCC‑GTTTTGAGAAACAACTGCCTATCCTGCCGACAACGGGAAACAGTaccggttcc                                                                                                                                                                                       >  3:812519/1‑268 (MQ=255)
                                                                                    gACA‑CCTCACGTGACGCAATCGAGCAAGAGGTGGTGTTGCACTGTGGGAGCGGCGACAGTTACGACGCGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCg                                                                                                                                                                                     <  1:805946/275‑1 (MQ=255)
                                                                                           tCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAATAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATgt                                                                                                                                                                               >  3:142579/1‑274 (MQ=255)
                                                                                             aCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                              <  2:393970/274‑1 (MQ=255)
                                                                                                    gcgATCGGGCAAGGTTGGGTGTTGCACTGTCGGAGCGGCGGCAGTTAGGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                   <  4:208810/278‑1 (MQ=255)
                                                                                                      gATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                         <  1:754740/238‑1 (MQ=255)
                                                                                                                         ttgccatGTCGGTGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAAGATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                               <  2:86866/271‑1 (MQ=255)
                                                                                                                         ttgaccggTCGGGGCGCCGGCAGTTACGACTTGCATTACAGTATTCGGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GAGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                               <  4:599308/270‑1 (MQ=255)
                                                                                                                          tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAAAC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAACACAGCGAAATGCCCTGGTGTTTTACGATCTTGATCGCTTTaa                                                                                                                                                 >  3:501493/1‑274 (MQ=255)
                                                                                                                           gCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACCACTGCGTATCATGCTGCAAACGGTAAACAGTACACATCAGCGCAATGCCCTGGTGTTTATCGATCTTGATCGCTTTATAGCgg                                                                                                                                            >  4:216562/1‑278 (MQ=255)
                                                                                                                            cACTGTCGGAGCGGCGGCAGTTACGACGTGCAATACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                   <  2:363622/238‑1 (MQ=255)
                                                                                                                             catGTTGGAGCGGCGGCAGTTAAGAGATGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGGGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCg                                                                                                                                             <  3:229355/273‑1 (MQ=255)
                                                                                                                             aCTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCaa                                                                                                                                                                                                            <  2:247866/212‑1 (MQ=255)
                                                                                                                                           ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGGGGTGAATGACAgggc                                                                                                                               >  1:335254/1‑275 (MQ=255)
                                                                                                                                           ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAgcgc                                                                                                                               <  1:126223/275‑1 (MQ=255)
                                                                                                                                           ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCCCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATTACAggcg                                                                                                                               >  3:33806/1‑272 (MQ=255)
                                                                                                                                                          cATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATAAGCAACATGCCCCGGGGTTTACCGATCTTGATCGCTTTAAACCGGTGAATGAAAGCGCCGCGCAGGCTGCGGGGGa                                                                                                             >  4:196083/1‑278 (MQ=255)
                                                                                                                                                          cATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAACTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                >  1:361201/1‑275 (MQ=255)
                                                                                                                                                           aTTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                <  3:401842/274‑1 (MQ=255)
                                                                                                                                                                  tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAACGGTGAAGTACAGCCCCGGGCAGGCGGCCGGTGACGCTTtgc                                                                                                     >  4:308600/1‑278 (MQ=255)
                                                                                                                                                                    ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTATCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtg                                                                                                      >  1:789747/1‑275 (MQ=255)
                                                                                                                                                                    ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GGTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTCCACATCAGCGACATGCCCTGGCGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCtt                                                                                                        >  1:342535/1‑273 (MQ=255)
                                                                                                                                                                     tACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTttctgc                                                                                                  >  2:319534/1‑278 (MQ=255)
                                                                                                                                                                        gCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGa                                                                                                                                                          <  3:625111/219‑1 (MQ=255)
                                                                                                                                                                         ccGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa                                                                                                                                                                                  >  3:525362/1‑194 (MQ=255)
                                                                                                                                                                         ccGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgctggg                                                                                                 >  1:190528/1‑273 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCa                                                                                                                                                                                                             >  4:104729/1‑164 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTACAAAGTACACATCATCGCCATGCCCTGGTGTTTATCGATGTTGAGCGCTTTACAGCGGTGGATGACAGCGCCGGCCATGCGGCTGGTGACCCttttcggtctgccgt                                                                                           >  4:363866/1‑266 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGCGATTCCGGACGTTACCGAATCACGCTAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGACGCACTGACGCATCGCGCCAACC‑GCGCCA‑GCTTTGAGAAACCACTGCGTATCCTGCCGCAAACGGTAAACAGTACACACCAGCGACATGCCCAGGCTTTTATCGATCTTGATCGCTTAAATGCGTGGAATGACAGCCCAGTGCATGCGGCCGGTGACGCCTTGCGGcgcgc                                                                                              >  3:806659/1‑274 (MQ=255)
                                                                                                                                                                             tAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCaa                                                                                                                                                                                                            <  2:104729/164‑1 (MQ=255)
                                                                                                                                                                             tAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                              <  1:525363/194‑1 (MQ=255)
                                                                                                                                                                               aGTACTCTGGACGGCAGCAATATGGTTTCGGTTCTGGTGATTCAGAACGTTACCGAAGCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCAATGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                        <  2:192451/278‑1 (MQ=255)
                                                                                                                                                                                 tACTCTGGACGGCAGCATAGTTTGTTCGTGTCTGGTGATTCAGGACGTTACCGACTCACGCAAAAGGCTGCGGCAGCTGAGCTACCGCGCCTACCATACTGCACTGACGCATCGCGCCAACC‑TCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTGGATCGCTTTAAAGCGGTGAATGACAGCGCCTGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                        <  2:221529/276‑1 (MQ=255)
                                                                                                                                                                                    tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTa                                                                                                                                                                    >  2:257161/1‑197 (MQ=255)
                                                                                                                                                                                     ctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                   <  4:257161/197‑1 (MQ=255)
                                                                                                                                                                                          cGGAAGCAATATTGGTTCGGTTCGGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGc                                                                             <  2:308600/278‑1 (MQ=255)
                                                                                                                                                                                           ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAAt                                                                               <  3:596120/275‑1 (MQ=255)
                                                                                                                                                                                                caATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt                                                                            <  1:728301/273‑1 (MQ=255)
                                                                                                                                                                                                           cggttcTGGTGATTCAGGACGTTACCGAATCACACAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCAGGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGGTGCGTAggctt                                                                  >  3:682620/1‑272 (MQ=255)
                                                                                                                                                                                                                  ggTGATTCGTGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCg                                                        <  1:469925/275‑1 (MQ=255)
                                                                                                                                                                                                                        tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGCTCTTGATCGCttt                                                                                                                                                   >  1:545717/1‑178 (MQ=255)
                                                                                                                                                                                                                        tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAAATGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTACTGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGATCTGGGGTCGTTACTCCTGGGTATGTTGCGTTCCAGGGACgcgc                                                  >  3:406954/1‑275 (MQ=255)
                                                                                                                                                                                                                            gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                               <  3:545715/178‑1 (MQ=255)
                                                                                                                                                                                                                             aCGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGgcg                                            >  3:610449/1‑275 (MQ=255)
                                                                                                                                                                                                                                   ccGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGGGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGt                                   <  2:270556/278‑1 (MQ=255)
                                                                                                                                                                                                                                        tCACGCAAACTGCTGGGCCAGCTGAGCGACAGCGCCTACCACGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCGTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGa                              <  2:220223/278‑1 (MQ=255)
                                                                                                                                                                                                                                        tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGt                                   >  3:292345/1‑273 (MQ=255)
                                                                                                                                                                                                                                        tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GCTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAACCAGTACACATCCGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTTAATGAAAGCCCCGGGCCTGCGGCGGGTTACGCTTTGCTTCCGTAACTGGCTTCGTTAATGCTGAGTTTGCTTGCTTCCCGCCACCTGCTTGGCGCG‑CCCGGTgg                                 >  3:338640/1‑275 (MQ=255)
                                                                                                                                                                                                                                           cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGGTCGGCCCGGGGGGGa                              >  3:710248/1‑275 (MQ=255)
                                                                                                                                                                                                                                           cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGGATCGgggggggt                             >  2:462169/1‑274 (MQ=255)
                                                                                                                                                                                                                                           cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTggg                                >  1:131912/1‑273 (MQ=255)
                                                                                                                                                                                                                                            gCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGa                              <  1:637917/274‑1 (MQ=255)
                                                                                                                                                                                                                                                     tgcGCCAGCTGAGCAACAGCGCAAACCCTGATGCACTAACGACTCGCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTACGTATCCTGCTACAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGt                    <  3:361201/275‑1 (MQ=255)
                                                                                                                                                                                                                                                     tgcCCCAACTGAGCTACAGCGCCTACCATGATGCACTAACGCATCTGGCCAACC‑GCGCCA‑GTTTTTAGAAACAACTGCGTCTCCTGCTGCAAACGGTAAACAGTACACATCAGCGCCATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGt                    <  3:183312/275‑1 (MQ=255)
                                                                                                                                                                                                                                                              tcggCTACCGCGCCTCCCATGTTGCACTGACGCATCGCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTa           <  1:681992/272‑1 (MQ=255)
                                                                                                                                                                                                                                                               gggctaaggcgcCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTAc          <  3:131912/267‑1 (MQ=255)
                                                                                                                                                                                                                                                                tgcgacaggcccTCCCAGGATGCAGTGAGGCATCGCCCCAACC‑GCGCCA‑GTTATGAGAAACAACCGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa      <  2:196083/268‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa        <  1:610451/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GGGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTAcc         <  3:468074/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa        <  1:533670/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tACTGCGCCTACCAATGTGGACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa      <  1:407515/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aGCGCCTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAACTTGGTCTGCCTCCACCAGa      >  1:530768/1‑272 (MQ=255)
                                                                                                                                                                                                                                                                       gtgcCTACCATGCAGCACTGACGCAACTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACTGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGt  <  1:748855/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ccTACCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTg                                  >  3:751541/1‑240 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                               
GAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACC‑GCGCCA‑GTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGC‑TGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGT  >  NC_000913/2145045‑2145581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A