Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A20 F33 I1 R1 9 139.8 3093418 86.2% 2666526 258.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA99.2% 432.0 / NA 134S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (67/66);  minor base G (1/0);  total (68/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145045‑2145584
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                
gaagCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                                                                                                                                                             <  1:543489/274‑1 (MQ=255)
       tAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACt                                                                                                                                                                                                                                                                      >  2:237656/1‑274 (MQ=255)
        aGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATGGGTTCGGTTCTGGTGAGTCAGGACGTTCCCGAATCACGCAAAATGCTGCCCCAGCTGAGCTCCAGCGCCTccccgaaacacgtc                                                                                                                                                                                                                                                                     >  4:612608/1‑263 (MQ=255)
             ttCCGCTCCTGACGGTGTTGCATATTACTTGTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAt                                                                                                                                                                                                                                                               <  2:554671/275‑1 (MQ=255)
                  cTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCAGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAACTCGc                                                                                                                                                                                                                                                          >  2:123016/1‑275 (MQ=255)
                     cTGACGGTGTTGCATATTACTTTTGGCGAGAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTACGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCCCTGACGCATCTCGCCaa                                                                                                                                                                                                                                                       >  2:76589/1‑275 (MQ=255)
                               tGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTT‑GTGTTTCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTCCCGACTCCCTCAAAATGCTTCGCCAGCTGAGCTACAGCGCCTACCCTTTTGCACTCACCCCTCCCGCCAACCTCGCCCGt                                                                                                                                                                                                                                             >  2:624788/1‑275 (MQ=255)
                                 ctaatTACTTTTGGCGACAACAGACAATTAATGGGTAACATTTAAAGGGCCGAAACCTCCCTGTCCGCGATCGAGCAAGATTTG‑GTGTTGCACGGTCGTAGCGGCGGCAGTTACGAATGGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGttt                                                                                                                                                                                                                                           <  3:267759/272‑1 (MQ=255)
                                      tACTTTTGGCGACAACGGACCATTAATGGGGAACATTTACAGTGCCGACACCTCACGTTACGCGAACGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTCAAGTACTCTGGACGGCAGAACTATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAAGCACGCAAAATGCTGCGCCAGCTGAGCTACCGCTCCCACCATGATGCACTGACGCAACTCCCCACCCCACCCAGtttctcg                                                                                                                                                                                                                                       >  3:79234/1‑270 (MQ=255)
                                        cTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgag                                                                                                                                                                                                                                       >  2:625170/1‑272 (MQ=255)
                                        cTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgag                                                                                                                                                                                                                                       >  1:627659/1‑272 (MQ=255)
                                          tttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGGGCTCAAGCGCCTACCATGATACACTTACGCATCTCGCCAACCCCGCCCGTTTTCAGaacc                                                                                                                                                                                                                                   >  4:311644/1‑272 (MQ=255)
                                           gtGGCGACAGAGGGCCCTTCATGGAGACCGTTTACACGGCCGACACCGCACGGTCCGCGATCGAGCAAGA‑GTGTGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaacaa                                                                                                                                                                                                                                 <  4:81945/274‑1 (MQ=255)
                                                        aCCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCCCCTACCATGAACCACTGCAGCTCCCCCCCAACCCCACCAGTTTTGAGAaacaacagagtcacctc                                                                                                                                                                                                                     >  4:197802/1‑263 (MQ=255)
                                                            ttAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                 <  3:627659/274‑1 (MQ=255)
                                                             tAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGCCGTGCATTACAGTATTACGCCGTTAAGTACTCTGGCCGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCACCTGCGCTACAGCGCCGACCATGATGCACTGACGCATCTGGCCAACCCCACCAGTTTTTAGAAACAAGTGCGtctacgtctgcca                                                                                                                                                                                                               >  3:56917/1‑263 (MQ=255)
                                                                  gagaACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGt                                                                                                                                                                                                          <  1:231904/275‑1 (MQ=255)
                                                                  gagaACAGTTACAGTGCCGACACCTCACGTTCGGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGTCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGt                                                                                                                                                                                                          <  2:125169/275‑1 (MQ=255)
                                                                      aCAGTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGCGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       <  1:266156/274‑1 (MQ=255)
                                                                       cATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGCTTCAGGACGTTACCGAATCCAGCAAAATTCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCACCCCCGCCATTTTTGACAAACAACTACGGATCCTTCTTCAAACCGTAAAc                                                                                                                                                                                                      >  4:502296/1‑274 (MQ=255)
                                                                          ttACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  2:181850/275‑1 (MQ=255)
                                                                          ttACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCAATACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  2:710500/275‑1 (MQ=255)
                                                                             cAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaac                                                                                                                                                                                                                                   <  2:520344/239‑1 (MQ=255)
                                                                                  ccGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAg                                                                                                                                                                                         >  3:688760/1‑275 (MQ=255)
                                                                                  acGACACCTCACGTTCCGCGTTCGAGCAAGATGTG‑GTGTTGAGCTGTCGGAACGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCCGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAGAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑Ca                                                                                                                                                                                          <  1:79234/273‑1 (MQ=255)
                                                                                     acacCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCACCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCTTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGGGa                                                                                                                                                                                      >  1:214930/1‑275 (MQ=255)
                                                                                         cTCACGTGCCGATATCGAACAAGTTGTG‑GTGTGGCACTGTCGGATCGGCGGCAGTTACGACAGGCATGACAGTACTACGCCGGTAAGTACTCGGGACGGAAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATg                                                                                                                                                                                  <  2:479036/275‑1 (MQ=255)
                                                                                            aCGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGccc                                                                                                                                                                               >  1:643238/1‑275 (MQ=255)
                                                                                                    cgATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGAAAAATGCTGCGCCACCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAAAGGTAAACAGTACACAT‑CAGCGACATGCACTGGTGtt                                                                                                                                                                        >  1:573348/1‑274 (MQ=255)
                                                                                                      aTCGAGCAAGGTGTG‑GTGTTGCACTGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGGATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                     <  2:98846/275‑1 (MQ=255)
                                                                                                                 tgtg‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATc                                                                                                                                                          >  1:305072/1‑275 (MQ=255)
                                                                                                                 tgtg‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGACGCACTGACGCATCTCGCCACCCGCGCCTGTTTTGAGAAACAACTGCGTATCCTTCCGCAAAAGGTACACAGTACACAA‑CAGCAACATCCTCAGGTGtttccagtgtttgata                                                                                                                                                          >  3:110606/1‑262 (MQ=255)
                                                                                                                  gtgtgtggtgCACTGTCGGTGCGGCGTCAGTGACGACGTGTATGTCAGTAGTACGCCGTTAAGTACTCTGGACGTCAGCAATATTGTGTCGGTTCTGGTGATTCAGGACGTTAGCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGATGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGAt                                                                                                                                                           <  4:564404/270‑1 (MQ=255)
                                                                                                                      gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTNTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATTATGCACTGACGCATCCCGCCAACCGCGCCAGTGTTGAGAAACAACTGCGTATCCTGCTGCAACCGGCAACCAGAACACAT‑CAGAGACATGCACTGGCGTTTATGGAACTTGATAGcat                                                                                                                                                      >  4:388908/1‑273 (MQ=255)
                                                                                                                          tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGCGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CCGCGACATTCCCTGGTGTTTATCGATCTTGATCCTTTTaaa                                                                                                                                                  >  3:380696/1‑275 (MQ=255)
                                                                                                                          tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                 <  3:235884/213‑1 (MQ=255)
                                                                                                                           gCACTGTCGGAGCGGCGGCAGTTACGACGTGCATGACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTTGTTCGGTTCTGGTGATTCAGGACGTTACCGACTCACGCAAAATGCTGCGCCAGCTGAACTACAGCGCCTACCATGCTGCCCTCACGCATCCCGCCCACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTTCAAACGGTCCACAGTCCACAC‑CAGCGGACTGCCCTTGTGTTTATTGATCATGATCGCTTTAAAg                                                                                                                                                 >  3:97719/1‑275 (MQ=255)
                                                                                                                            cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCCCTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTACCCTGCTTCAAACGGCAACCAGTACACCT‑CAGCAACATGACCCGGTGGTTATCGATATTTCCCGCTTTaatg                                                                                                                                                 >  4:479647/1‑272 (MQ=255)
                                                                                                                            cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCttt                                                                                                                                                     >  2:563417/1‑270 (MQ=255)
                                                                                                                            cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                 >  1:474778/1‑274 (MQ=255)
                                                                                                                            cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGAGCCTACAATGATGCACTGACGCATCCCGCCAAACCCGCCAGTTTTGAGAAATAACTTCATTCCCTCCTCCAAACGGTAAACAGTAAACATACAG‑GAACTCCCCTGGGGGTTATAGATGTTGATGCCTTTTAAg                                                                                                                                                 >  4:627185/1‑274 (MQ=255)
                                                                                                                              cTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCAT‑CAGCGACATGCCCTGGTGTTTATCGATCGTGAGCGCTTTAAAGCgg                                                                                                                                              >  4:21099/1‑275 (MQ=255)
                                                                                                                                gccggagcggagGCAGTTCCGGCTTACATTACAGTATTGCGCCGTTAAGTACTCTGAACGGCAGCAATATTGGTTCGGTTCGGGTGAGTCACGACGGTACCGAATCACACAAAGTGCTGCGCCAGCTGAGCTACGGCGAATACCATGAATCACTGACGAATCTCGCCAACCGCGGCAGTTTGGAGAAACAACTGCGTATCCTGCTGCAAACGGTGAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAGAGCGGTg                                                                                                                                            <  3:631377/273‑1 (MQ=255)
                                                                                                                                 tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCCACCATGATGCACTGAAGCATCTCGCCAACCGCGCCAGTTTTGAGAAAAAACTGCGTATCCTGCTGCAAACGGTAAACCGTACACTT‑CAGCGACATGACCTGGTGTTTATCGATCTTGTTCGCTTTAACGCGGTg                                                                                                                                            >  1:362453/1‑274 (MQ=255)
                                                                                                                                                           aTTACCGTATTACTCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                  <  2:523085/274‑1 (MQ=255)
                                                                                                                                                                  tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCAAC‑CAGCGACATGCCCTGGTGTTTATCAACCTTGCACGTTTTAAATCGGAGAAGGACAGCGTCGGGCCAGCGGCGGGTGAAGCtt                                                                                                          >  4:198745/1‑275 (MQ=255)
                                                                                                                                                                  tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTTACGCtt                                                                                                          >  2:449769/1‑275 (MQ=255)
                                                                                                                                                                    ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGTGTGACGCTTtg                                                                                                        >  1:649177/1‑275 (MQ=255)
                                                                                                                                                                       cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCAT‑CAGCGACATTCCCTGGTGTTTATCGATCTTGATCGTTTTAAAGCGGTGAATGACAGCGCCGGCCATTCGGCGGGTGACGCTTtgctg                                                                                                     >  1:81105/1‑275 (MQ=255)
                                                                                                                                                                       cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGCTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCCGCTGAGCTACAGCGCCTACCATTATGCACTGACGCATCCCGCCCACCGCGCCAGGTTTGAGAAACAACTGAGTATCCTGCTGCAAACGGTATACAGTACAAAT‑CAGCCACACGCCCTGGTGTTTATCGTTCTTGATTGCTTTAAAGCGTTTAATGACTGCCCCGGGATTGCCGCGGGTGACGCTTtgctg                                                                                                     >  3:317567/1‑275 (MQ=255)
                                                                                                                                                                           gTTAAGTACACAGGACGGGAGCAGTATTGATTCCGTTCTGGGGATTCAAGACGTTACCGAAGCACGCAAAAAGCTGCCCCAGCTGAGCTACAGCGCCTACCATGCTGCACTGCCGCATCTCGCCGACCGCGCCAGTATTGAGAAACAACTGCGTATCCTGCTGCAAACGGGAAGCTGTACACAT‑CAGCGACATGCCCTGGTGTTGATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTATGCTGcgcg                                                                                                 <  3:40195/275‑1 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTTTGGTGATTCAGGACGTTACCGAATAACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCTTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGCTCGCTTTAAAGCGGTGAATGACAGCGCCGGTCATGCGGCGGGTGACGCTTTGCTCCGCGa                                                                                                >  1:306202/1‑275 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACa                                                                                                                                                                                    >  1:238632/1‑191 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa                                                                                                >  1:530024/1‑275 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAC‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGTTGACGCTGTGCTGCGCGa                                                                                                >  2:557564/1‑275 (MQ=255)
                                                                                                                                                                            ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACAGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTAGCCAACAGCGCCACTTTTGAGAAACAACTGCGTATCCTGCTGCTAACGGTCAACAGTAAACAT‑CAACCACCCCCCCTGGTTGTTATAGAACGTGAACCCTTTCAAGCGGTGAATGACCGCACCTGGATTTCGGCGGGTGCAGCTTTGgcgcgcac                                                                                                >  4:328594/1‑273 (MQ=255)
                                                                                                                                                                                gTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGcc                                                                                                                                                                                <  3:238632/191‑1 (MQ=255)
                                                                                                                                                                                  acccttgACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGGTACCGAATCCCGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  3:474778/269‑1 (MQ=255)
                                                                                                                                                                                  aCTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  2:21099/275‑1 (MQ=255)
                                                                                                                                                                                  aCTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  3:305072/275‑1 (MQ=255)
                                                                                                                                                                                    tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgt                                                                                        >  1:733628/1‑275 (MQ=255)
                                                                                                                                                                                    tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACAGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CATCGAAATAACCGGGTGTTTATCGATCTTGATCGCTTTAAATCGTTGAATGACAGCGCCGGGCGTGCGGCGGGGGACTGTTGGTTGCGGGAACTGAcgg                                                                                        >  4:521108/1‑274 (MQ=255)
                                                                                                                                                                                      tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACTGCGCCGGGCCTGCGGCGTGTGCCGTTTGGCTGCCCGGACttgggtgg                                                                                      >  3:290295/1‑268 (MQ=255)
                                                                                                                                                                                      tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCCCCAGCTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGCGAATGAAAGCGCCGGGCATGCGGCGGGTGCCGCATTGCTGCGGGAACTGGcg                                                                                         >  2:153761/1‑272 (MQ=255)
                                                                                                                                                                                           ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAAt                                                                                 <  3:530024/275‑1 (MQ=255)
                                                                                                                                                                                             cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGc                                                                               <  3:643238/275‑1 (MQ=255)
                                                                                                                                                                                             cagcaATATTGGTTCGGTTCTGGTGATGCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCGACAGCGCCTACCATGATGCACTGACGCATATCGCCAACCGCGCCAGTATTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGAGCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGc                                                                               <  2:460902/275‑1 (MQ=255)
                                                                                                                                                                                              agcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt                                                                              <  3:79482/275‑1 (MQ=255)
                                                                                                                                                                                                caATATTGTTGCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt                                                                              <  2:521108/273‑1 (MQ=255)
                                                                                                                                                                                                caATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTg                                                                             <  2:612608/274‑1 (MQ=255)
                                                                                                                                                                                                       ggttcggttcTTGCAATTCAAGACGTTACCGAACCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgct                                                                     <  3:561898/275‑1 (MQ=255)
                                                                                                                                                                                                        gttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAACTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTCTCCTGCTGAAAACGGTCAACAGTACACAT‑CAGCGACATTCCCTGGTGTTTATCGAACTTGATAGCTTTAAAGGGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGGGCGAAATGGGGTCGTGAATGCAGAGTGtgctg                                                                    >  4:99214/1‑275 (MQ=255)
                                                                                                                                                                                                        gttcggttcTAGTGGTTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCGACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgct                                                                     <  1:110606/274‑1 (MQ=255)
                                                                                                                                                                                                           cggttcGAGTAACTCAGGACATTACCGAATCACGAAAAAGGACGGGCCAGCATAGGGACAGCGCCTACCATGATGAACTGACGCATGTCGCCAACCGGACCAGTGTTGAGAGACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGTCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCATTAATGCTGAGTATGCTGCg                                                                  <  3:15462/274‑1 (MQ=255)
                                                                                                                                                                                                             gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACTGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGGTAATGCTGAGTATGCTGCGtgc                                                               >  1:146556/1‑273 (MQ=255)
                                                                                                                                                                                                             gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCTTCGTTAATGCTGGGTATGCGGCGTTc                                                               >  1:675423/1‑275 (MQ=255)
                                                                                                                                                                                                              ttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTg                                                                             <  1:138165/260‑1 (MQ=255)
                                                                                                                                                                                                                  ggTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCg                                                          <  1:53017/275‑1 (MQ=255)
                                                                                                                                                                                                                        tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGGACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGc                                                    >  1:93417/1‑275 (MQ=255)
                                                                                                                                                                                                                        tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTg                                                     <  1:106769/274‑1 (MQ=255)
                                                                                                                                                                                                                                 tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCCCGAc                                           >  1:8121/1‑275 (MQ=255)
                                                                                                                                                                                                                                           cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCttt                                                                                                                                                     >  2:152787/1‑159 (MQ=255)
                                                                                                                                                                                                                                           cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCCTTGCTGCGCGAACTGGCGTCGTGAATACTGAGTATGCTGCGTTCCCGCGACGTGATGGCGCGACGCGggggggg                                 >  3:67318/1‑274 (MQ=255)
                                                                                                                                                                                                                                            gCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                 <  2:388908/274‑1 (MQ=255)
                                                                                                                                                                                                                                            gCAAAATGCTGCGCCAACGTAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGACAGTTGTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                 <  2:208203/274‑1 (MQ=255)
                                                                                                                                                                                                                                            acAAAATGCTGCGCCAGCTGAGCTACAGCGCATACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACGGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCGTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                 <  2:422457/273‑1 (MQ=255)
                                                                                                                                                                                                                                             cAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGAt                                >  1:442275/1‑274 (MQ=255)
                                                                                                                                                                                                                                               aaaTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                 <  4:152787/159‑1 (MQ=255)
                                                                                                                                                                                                                                               aaaTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  2:295404/214‑1 (MQ=255)
                                                                                                                                                                                                                                                     tgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt                       <  3:510144/275‑1 (MQ=255)
                                                                                                                                                                                                                                                     tgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt                       <  1:56917/275‑1 (MQ=255)
                                                                                                                                                                                                                                                      gcgcCTGCTGAGCTACCGCCCCCACCATGATACACTGACGCAGCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CCGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTc                      <  4:123016/275‑1 (MQ=255)
                                                                                                                                                                                                                                                      gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGACGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCCGTGACGTGCTGGCGCGACTCGGGTGGGATGAATCTggg                       >  3:539722/1‑273 (MQ=255)
                                                                                                                                                                                                                                                       cgcCGGCTGAGCGACAGCGCCTACCATGCTGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTACAAACGGTAAACAGTACACAT‑GAGCGACATGCCCTGGTGGTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTct                     <  3:139069/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         ccAGTTGAGAAAAAGCGCCTACCAGGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTGGAGAACCAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAG‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  2:328594/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         ccAGCTGGGCTACAGCGCCTACCATGATGCACTGACGCAGCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCGGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  1:393443/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         ccAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTct                     <  1:245352/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gccaaagggcCTACCATGATGCACTGACCCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa           <  3:8121/267‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATACTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa           <  1:67318/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ccTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAt  >  2:266478/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                
GAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTG‑GTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAT‑CAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145045‑2145584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A