Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A51 F1 I1 R1
|
20 |
58.0 |
3778966 |
96.2% |
3635365 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,035,629 |
(C)5→6 |
coding (228/768 nt) |
wbbD ← |
putative glycosyltransferase WbbD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,035,624 | 1 | . | C | 91.1%
| 217.6
/ 13.6
| 56 | coding (233/768 nt) | wbbD | putative glycosyltransferase WbbD |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base C (28/23); total (30/26) |
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 |
TTCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATA > REL606/2035550‑2035692
|
ttCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑c > 1:1737544/1‑76 (MQ=255)
ttCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑c < 1:1536907/76‑1 (MQ=255)
tCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cc > 2:485960/1‑76 (MQ=255)
aTTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑ccccc > 2:687372/1‑76 (MQ=255)
aTTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑ccccc > 1:801814/1‑76 (MQ=255)
aTTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑ccccc > 2:1799269/1‑76 (MQ=255)
tttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccc < 2:4159/76‑2 (MQ=255)
tttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccc < 2:217125/76‑2 (MQ=255)
ttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccca < 2:1040859/76‑3 (MQ=255)
ttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccca > 1:1368123/1‑74 (MQ=255)
ttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccca < 2:681579/76‑3 (MQ=255)
atcatcAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCCAaccc < 1:1603664/76‑1 (MQ=255)
caGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCatat > 1:1803884/1‑76 (MQ=255)
caGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCatat > 1:1145812/1‑76 (MQ=255)
caGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCatat > 1:602909/1‑76 (MQ=255)
aGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATAtt > 2:847485/1‑76 (MQ=255)
aGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATAtt > 1:510911/1‑76 (MQ=255)
aaTCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATAtttt > 1:1665062/1‑76 (MQ=255)
gCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCa < 1:1038077/76‑1 (MQ=255)
gCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCa < 2:492892/76‑1 (MQ=255)
tATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAgtgt > 1:1818635/1‑76 (MQ=255)
tATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAgtgt > 1:1881229/1‑76 (MQ=255)
tATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAgtgt > 2:335678/1‑76 (MQ=255)
aTTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGtgtg > 1:1410562/1‑76 (MQ=255)
aTTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGtgtg > 1:1239991/1‑76 (MQ=255)
aTTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGtgtg < 1:913835/76‑1 (MQ=255)
tACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGan < 1:938253/76‑2 (MQ=255)
tACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGan < 1:1385539/76‑2 (MQ=255)
tACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGaa < 1:741643/76‑1 (MQ=255)
cTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAAt < 2:1235257/76‑1 (MQ=255)
tAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATc < 1:58237/76‑1 (MQ=255)
aaaTCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCtt < 2:440764/76‑1 (MQ=255)
tCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCtttt < 1:363625/76‑1 (MQ=255)
cATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTta > 1:649432/1‑76 (MQ=255)
cATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTNta < 2:1333582/76‑1 (MQ=255)
aTGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtat < 1:750806/76‑1 (MQ=255)
aTGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata > 1:1526854/1‑76 (MQ=255)
tGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata > 2:787300/1‑76 (MQ=255)
tGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata < 1:1713281/76‑1 (MQ=255)
tGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata < 1:1665601/76‑1 (MQ=255)
gCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTatat > 1:968744/1‑76 (MQ=255)
cATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATAtt > 1:1709027/1‑76 (MQ=255)
aCATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTaa < 2:1818635/76‑1 (MQ=255)
cATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTaaa < 1:572351/76‑1 (MQ=255)
cATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTaaa < 1:888879/76‑1 (MQ=255)
tGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGCGTGAAATCTTTTATATTTAAatat > 1:1202868/1‑76 (MQ=255)
aTTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtct < 1:1817111/76‑1 (MQ=255)
ttCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtctt > 2:1297013/1‑76 (MQ=255)
ttCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtctt < 2:1476726/76‑1 (MQ=255)
cAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtcttct > 1:472493/1‑76 (MQ=255)
aaCCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTtctc > 2:1497944/1‑76 (MQ=255)
aaaaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACt < 1:715620/76‑1 (MQ=255)
aaaaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACt < 1:1211926/76‑1 (MQ=255)
aaaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTa > 1:387952/1‑76 (MQ=255)
aaaTTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTAt > 2:1064778/1‑76 (MQ=255)
aaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTAt < 1:805587/76‑1 (MQ=255)
tttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCa > 2:1631112/1‑76 (MQ=255)
tttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCa > 1:1075068/1‑76 (MQ=255)
ttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCaa > 1:1747270/1‑76 (MQ=255)
ttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCaa > 2:230617/1‑76 (MQ=255)
ttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCaa > 1:195345/1‑76 (MQ=255)
tAACGCTGCCCCCCAACCCCATATTTTTTTCACGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAAt > 2:595991/1‑76 (MQ=255)
tAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAAt > 1:1866278/1‑76 (MQ=255)
tAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAAt < 1:94853/76‑1 (MQ=255)
tAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATa < 2:1589369/76‑1 (MQ=255)
aaCGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATa < 1:306904/76‑1 (MQ=255)
aaCGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATa > 1:1211232/1‑76 (MQ=255)
|
TTCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATA > REL606/2035550‑2035692
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
