Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A52 F1 I1 R1
|
19 |
66.0 |
4263516 |
97.2% |
4144137 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,911,997 |
(A)6→7 |
intergenic (‑198/‑125) |
yifB ← / → ilvL |
predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,911,991 | 1 | . | A | 98.3%
| 231.7
/ ‑2.6
| 59 | intergenic (‑192/‑131) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (29/29); total (30/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 |
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAT > REL606/3911916‑3912061
|
gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt < 2:333006/76‑1 (MQ=255)
gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt > 1:315239/1‑76 (MQ=255)
gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt < 1:2087315/76‑1 (MQ=255)
aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑a < 1:1302911/76‑1 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 2:1908056/1‑76 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 1:365532/1‑76 (MQ=255)
aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaa < 1:1743754/76‑1 (MQ=255)
ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaa < 2:1064986/76‑1 (MQ=255)
aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaaac > 2:66514/1‑74 (MQ=255)
cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAcaacaa < 2:1739631/76‑1 (MQ=255)
cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAcaacaa > 2:1523211/1‑76 (MQ=255)
tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAAt < 1:228411/76‑1 (MQ=255)
aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAAtt < 1:1710520/76‑1 (MQ=255)
cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAAttt < 1:1480558/76‑1 (MQ=255)
aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTtat < 2:622733/76‑1 (MQ=255)
aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTtat > 2:135730/1‑76 (MQ=255)
aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt > 2:1148646/1‑76 (MQ=255)
aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt > 1:1193086/1‑76 (MQ=255)
aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt > 1:685383/1‑76 (MQ=255)
aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTg < 2:1280803/76‑1 (MQ=255)
aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTg < 1:1146436/76‑1 (MQ=255)
aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTg > 1:578701/1‑76 (MQ=255)
gggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGa > 2:560961/1‑76 (MQ=255)
gggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGa > 1:997192/1‑76 (MQ=255)
ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaa < 1:152163/76‑1 (MQ=255)
ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaa < 1:128696/76‑1 (MQ=255)
gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaaa < 2:1563267/76‑1 (MQ=255)
gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaaa > 2:1704356/1‑76 (MQ=255)
gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaaa > 1:1509676/1‑76 (MQ=255)
aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat < 1:596272/76‑1 (MQ=255)
aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat > 2:1531200/1‑76 (MQ=255)
aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat < 1:1903041/76‑1 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattatt < 1:920796/76‑1 (MQ=255)
caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAAttatta > 1:1837755/1‑76 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa < 1:411020/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa > 1:1214293/1‑76 (MQ=255)
tCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAAc > 2:1614573/1‑76 (MQ=255)
cATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:331276/76‑1 (MQ=255)
cTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 2:707909/1‑76 (MQ=255)
aaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:732758/76‑1 (MQ=255)
aaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:2079423/76‑1 (MQ=255)
aaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:2077201/76‑1 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:1696593/76‑1 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 2:2000432/1‑76 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATTATaaaa > 2:2121544/1‑76 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:1850672/76‑1 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:373415/76‑1 (MQ=255)
tGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa < 1:1933114/76‑1 (MQ=255)
aTTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCAaaaaaaatc < 2:1759761/76‑4 (MQ=255)
tttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc > 1:427710/1‑76 (MQ=255)
tttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc < 2:1349356/76‑1 (MQ=255)
tttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc < 1:1416966/76‑1 (MQ=255)
ttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa > 2:119387/1‑76 (MQ=255)
tttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaa > 1:567765/1‑76 (MQ=255)
ttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa < 1:443702/76‑1 (MQ=255)
ttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa > 1:1800273/1‑76 (MQ=255)
tcactcacTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa < 2:250035/76‑1 (MQ=255)
tcactcacTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa > 2:86019/1‑76 (MQ=255)
cactATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAatat > 2:1709125/1‑76 (MQ=255)
ctATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 1:1729068/1‑76 (MQ=255)
tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 1:1080372/1‑76 (MQ=255)
tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 1:991572/1‑76 (MQ=255)
attttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg > 1:1716929/1‑76 (MQ=255)
attttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg > 1:1312957/1‑76 (MQ=255)
tttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa < 2:1640893/76‑1 (MQ=255)
tttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa < 1:1670182/76‑1 (MQ=255)
ttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:790330/1‑76 (MQ=255)
ttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:352917/1‑76 (MQ=255)
tattttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt < 1:1674387/73‑1 (MQ=255)
attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa < 2:1808768/74‑1 (MQ=255)
attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:503413/3‑76 (MQ=255)
attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:373999/3‑76 (MQ=255)
attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:1054877/3‑76 (MQ=255)
attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa < 2:977482/74‑1 (MQ=255)
ttttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt > 2:1246920/2‑76 (MQ=255)
ttttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt > 1:659705/2‑76 (MQ=255)
|
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAT > REL606/3911916‑3912061
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
