Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A68 F1 I1 R1
|
17 |
56.4 |
3914180 |
96.2% |
3765441 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,911,997 |
(A)6→7 |
intergenic (‑198/‑125) |
yifB ← / → ilvL |
predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,911,991 | 1 | . | A | 97.7%
| 174.1
/ ‑2.7
| 44 | intergenic (‑192/‑131) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (20/23); total (20/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 |
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATT > REL606/3911916‑3912062
|
gACAAAGGATGAACTACGAGGAAGGGAACAACCTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt > 1:1076001/1‑76 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 2:78308/1‑76 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 2:1738686/1‑76 (MQ=255)
aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa < 2:247174/76‑1 (MQ=255)
aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaa < 2:1138617/76‑1 (MQ=255)
gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaaa < 2:1535404/76‑2 (MQ=255)
aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaaac < 2:1125691/76‑3 (MQ=255)
aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAacaaca < 1:1333651/76‑1 (MQ=255)
aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAacaaca > 2:442919/1‑76 (MQ=255)
gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTta > 1:355878/1‑76 (MQ=255)
aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTtat < 1:820366/76‑1 (MQ=255)
aGGAAGGGAACAACATTCAGACTGAAATTTAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTgat < 2:487352/76‑1 (MQ=255)
aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt < 1:340692/76‑1 (MQ=255)
acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAatt > 2:238319/1‑76 (MQ=255)
acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAatt < 2:1814805/76‑1 (MQ=255)
caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAatta < 2:76653/76‑1 (MQ=255)
aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat < 2:1391311/76‑1 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattatt > 2:1172100/1‑76 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattatt < 2:1588914/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa > 1:1730398/1‑76 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa > 1:1405010/1‑76 (MQ=255)
ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaaa > 2:740408/1‑76 (MQ=255)
ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaaa < 2:918810/76‑1 (MQ=255)
tCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAAc > 1:831259/1‑76 (MQ=255)
cATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACg > 1:1189169/1‑76 (MQ=255)
aTACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGc > 2:1414835/1‑76 (MQ=255)
aCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:892557/1‑76 (MQ=255)
aCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:1742329/1‑76 (MQ=255)
tGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatca > 2:1106339/1‑76 (MQ=255)
tGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatca < 2:1685528/76‑1 (MQ=255)
gAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:1106476/76‑1 (MQ=255)
aaaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 1:50455/76‑1 (MQ=255)
aaaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:231299/76‑1 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:994668/76‑1 (MQ=255)
aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:657872/1‑76 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:1578208/76‑1 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:644705/76‑1 (MQ=255)
tGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt < 1:1659183/76‑1 (MQ=255)
gAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt > 1:1850033/1‑76 (MQ=255)
aaTTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc < 1:941947/76‑1 (MQ=255)
ttttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGc > 1:24110/1‑76 (MQ=255)
tcactcacTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa < 1:841620/76‑1 (MQ=255)
cactcactATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa < 2:719614/76‑1 (MQ=255)
tctcactATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaaa > 2:1913475/2‑76 (MQ=255)
ctcactATTCTATTTTT‑AACAAACAACACTTTATATTGAAATTATTAAACGCATCCTAACAATCAGCCACCAAata > 2:914301/1‑76 (MQ=255)
ctATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 1:948976/1‑76 (MQ=255)
tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 2:1773267/76‑1 (MQ=255)
tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 2:1640022/1‑76 (MQ=255)
tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 1:1464794/76‑1 (MQ=255)
attttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg < 1:152443/76‑1 (MQ=255)
ttttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 1:1592461/76‑1 (MQ=255)
tttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1267653/1‑76 (MQ=255)
tttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt < 1:292058/76‑1 (MQ=255)
tttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt < 2:905047/76‑1 (MQ=255)
|
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATT > REL606/3911916‑3912062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
