mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 430,835 | C→T | intergenic (‑48/‑108) | insL‑2 ← / → lon | putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La |
| RA | 474,437 | C→T | G290E (GGG→GAG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| MC | 547,700 | Δ42,623 bp | between IS1 | [nmpC]–[insK‑1] | 44 genes [nmpC], ybcR, ybcS, ybcT, ybcU, ECB_00510, nohB, ECB_00512, ECB_00513, ECB_00514, ECB_00515, ECB_00516, ECB_00517, appY, ompT, envY, ybcH, nfrA, nfrB, nfrB, ECB_00524, yhhI, ECB_00526, ECB_00527, ECB_00528, ECB_00529, ECB_00530, cusS, cusR, cusC, ylcC, cusB, cusA, pheP, ybdG, nfnB, ybdF, ybdJ, ybdK, insJ‑1, insK‑1, insB‑7, insA‑7, [insK‑1] |
| RA | 649,520 | T→C | N428D (AAC→GAC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| JC | 1,476,358 | Δ13 bp | coding (77‑89/537 nt) | ydcN → | predicted DNA‑binding transcriptional regulator |
| RA | 1,610,902 | T→C | intergenic (‑42/+322) | cspI ← / ← ydfP | cold shock protein/conserved protein |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| MC JC | 2,031,850 | Δ22,863 bp | [manB]–wcaJ | 22 genes [manB], manC, insB‑14, insA‑14, wbbD, wbbC, wzy, wbbB, wbbA, vioB, vioA, wzx, rmlC, rfbA, rfbD, rfbB, galF, wcaM, wcaL, wcaK, wzxC, wcaJ |
|
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| JC | 2,298,585 | +ACG | coding (498/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| RA | 2,718,725 | A→G | F161L (TTT→CTT) | ygaD ← | competence damage‑inducible protein A |
| RA | 3,570,803 | Δ1 bp | coding (4/435 nt) | uspA → | universal stress global response regulator |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
| RA | 3,911,997 | (A)6→7 | intergenic (‑198/‑125) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| RA | 4,161,106 | A→G | E84G (GAA→GGA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 4256585 = | 73 (1.080) | 51 (0.780) | 42/146 | 0.5 | 43.6% | coding (184/465 nt) | soxR | DNA‑binding transcriptional dual regulator, Fe‑S center for redox‑sensing |
| ? | REL606 | = 4375294 | 61 (0.930) | coding (1075/1338 nt) | amiB | N‑acetylmuramoyl‑l‑alanine amidase II | |||||