Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A83 F1 I1 R1
|
17 |
39.7 |
2446701 |
98.6% |
2412447 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,920,708 |
G→A |
A151V (GCG→GTG) |
znuA ← |
high‑affinity zinc transporter periplasmic component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,920,708 | 0 | G | A | 97.4%
| 139.9
/ ‑6.5
| 38 | A151V (GCG→GTG) | znuA | high‑affinity zinc transporter periplasmic component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/18); minor base C (1/0); total (20/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
GGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTT > REL606/1920635‑1920772
|
ggCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACt < 2:1076492/76‑1 (MQ=255)
cGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAt < 1:1154716/76‑1 (MQ=255)
cGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAt < 2:822234/76‑1 (MQ=255)
cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc < 2:610241/76‑1 (MQ=255)
cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc < 2:610235/76‑1 (MQ=255)
cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc < 1:277622/76‑1 (MQ=255)
cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc > 1:687564/1‑76 (MQ=255)
aaGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATctct < 2:782236/76‑1 (MQ=255)
gtgtGGCTCGACTTTGTGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTgg < 1:201474/73‑1 (MQ=255)
gTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTgg < 1:201490/76‑1 (MQ=255)
tttGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTggg < 2:1065980/76‑1 (MQ=255)
ggCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGaa < 2:898378/76‑1 (MQ=255)
ggCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGaa < 2:1010468/76‑1 (MQ=255)
cGACTTTGCGGCATAAGTTCCACTAATTTTCCATGAATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGcc > 1:124725/1‑76 (MQ=255)
cGACTTTGCGGCATAAGTTCCACTAATTTTCCATGAATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGcc > 1:124717/1‑76 (MQ=255)
tttGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAg < 1:647097/76‑1 (MQ=255)
tGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCCCTATCTCTGGGGAAAGCCAAAGAt > 2:824033/1‑76 (MQ=255)
tGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGAt > 2:824038/1‑76 (MQ=255)
tGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGAt < 1:986332/76‑1 (MQ=255)
cGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGc > 2:535237/1‑76 (MQ=255)
tAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGtt < 1:630702/76‑1 (MQ=255)
tAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGtt > 1:470763/1‑76 (MQ=255)
aaGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTg > 1:281213/1‑76 (MQ=255)
aaGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTg > 2:1086574/1‑76 (MQ=255)
aGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGa > 1:1175480/1‑76 (MQ=255)
ttCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGaaa > 1:280643/1‑76 (MQ=255)
cACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCg < 1:155554/76‑1 (MQ=255)
tAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCg > 2:1058270/1‑76 (MQ=255)
ttttCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGa > 1:995897/1‑76 (MQ=255)
ccATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGt > 1:221443/1‑76 (MQ=255)
aTGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGa < 1:276776/76‑1 (MQ=255)
tGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGAt > 2:654600/1‑76 (MQ=255)
tGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGAt < 1:1042495/76‑1 (MQ=255)
gATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCt > 1:647747/1‑76 (MQ=255)
tGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCg > 2:1216375/1‑76 (MQ=255)
tGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCg > 1:340641/1‑76 (MQ=255)
tGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCg > 1:340633/1‑76 (MQ=255)
aaCCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCa > 1:462778/1‑76 (MQ=255)
cTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACtttt > 2:738636/1‑76 (MQ=255)
cTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACtttt > 1:577049/1‑76 (MQ=255)
|
GGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTT > REL606/1920635‑1920772
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
