Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A33 F1 I1 R1
|
22 |
54.4 |
3455062 |
95.8% |
3309949 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,031,943 |
C→T |
P285P (CCG→CCA) |
manB ← |
Phosphomannomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,031,943 | 0 | C | T | 100.0%
| 62.0
/ NA
| 19 | P285P (CCG→CCA) | manB | Phosphomannomutase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/11); total (8/11) |
CGTGTCCGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGCGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCG > REL606/2031870‑2031979
|
cGTGTCCGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTgg < 2:256442/76‑1 (MQ=255)
cGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTg < 2:764860/76‑1 (MQ=255)
tttCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGgatg < 1:1236849/76‑1 (MQ=255)
ttCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGgatga < 1:855600/76‑1 (MQ=255)
gACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCt > 1:1691462/1‑76 (MQ=255)
gACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCt > 1:563605/1‑76 (MQ=255)
cATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTc < 2:43784/76‑1 (MQ=255)
aTCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTcg < 2:845337/76‑1 (MQ=255)
cACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTcgcg > 2:546638/1‑76 (MQ=255)
ccGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGGGGGTGATCTTCGCGcc > 2:1643426/1‑76 (MQ=17)
cGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCg > 2:1089160/1‑76 (MQ=255)
ggAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCgg > 2:1114561/1‑76 (MQ=255)
ggAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCCCCgg > 2:1622598/1‑76 (MQ=255)
gAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCggg > 2:434876/1‑76 (MQ=255)
gAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCggg < 2:909376/76‑1 (MQ=255)
aGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGa > 1:1553050/1‑76 (MQ=255)
tGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGAtt < 2:1432378/76‑1 (MQ=255)
gccgccCGCGGCGGTCACCACATCAAAGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGAttt > 2:592451/1‑76 (MQ=14)
gccgccCGCGGCGGGCACCACATCACAGGTGATCCAGGAACGACGTGGATCGTGGCTGATCTTCGCGCCGGGCttt > 2:22207/1‑76 (MQ=14)
cgcgGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCg < 1:1147145/76‑1 (MQ=25)
cgcgGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCg < 1:535819/76‑1 (MQ=25)
cgcgGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCg < 1:535801/76‑1 (MQ=25)
|
CGTGTCCGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGCGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCG > REL606/2031870‑2031979
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
