Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A33 F1 I1 R1
|
22 |
54.4 |
3455062 |
95.8% |
3309949 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,175,323 |
G→T |
N342K (AAC→AAA) |
uxaA ← |
altronate hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,175,323 | 0 | G | T | 100.0%
| 106.8
/ NA
| 29 | N342K (AAC→AAA) | uxaA | altronate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (18/11); total (18/11) |
GCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAA > REL606/3175252‑3175397
|
gCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTtttt < 1:185705/76‑1 (MQ=255)
tttCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCa > 2:1309300/1‑76 (MQ=255)
ttCTGGTTACAGCCAAGTGATTTGTCTTCAAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCAt < 2:299362/76‑1 (MQ=255)
tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATa > 1:928609/1‑76 (MQ=255)
tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATa > 1:381424/1‑76 (MQ=255)
tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATa > 1:2557/1‑76 (MQ=255)
tGGGTACAGCCAAGTGATTTGTCTTCTAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGa < 2:485219/76‑1 (MQ=255)
tGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGa > 1:1123575/1‑76 (MQ=255)
gggTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGAt > 2:612621/1‑76 (MQ=255)
gTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCg < 1:68811/76‑1 (MQ=255)
cAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCt > 2:27792/1‑76 (MQ=255)
ccAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGac > 1:643271/1‑75 (MQ=255)
ccAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGAt < 2:1427615/76‑1 (MQ=255)
cAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATc > 2:1222627/1‑76 (MQ=255)
tGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATgg > 2:1287686/1‑76 (MQ=255)
aTTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGc > 1:612033/1‑76 (MQ=255)
ttGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCaa > 2:1363102/1‑76 (MQ=255)
tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAAt > 1:1331880/1‑76 (MQ=255)
tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAAt > 2:1226900/1‑76 (MQ=255)
tCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATaa < 1:1134349/76‑1 (MQ=255)
tCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATaa < 1:980836/76‑1 (MQ=255)
cTTCCAGCGTGGTGATACCGCCCGCTTTGTTCTCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATaaa > 1:225440/1‑76 (MQ=255)
cAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTAc > 2:802968/1‑76 (MQ=255)
aGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACt > 2:1491250/1‑76 (MQ=255)
aGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCACGGGCAATAAAGTACt > 1:370310/1‑76 (MQ=255)
gATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGACTGATCATGGGCAATAAAGTACTGTTTGAAg < 1:855751/76‑1 (MQ=255)
tttGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATg > 1:1603518/1‑76 (MQ=255)
tttGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATg < 1:1514556/76‑1 (MQ=255)
tGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGt < 2:883071/76‑1 (MQ=255)
ccccGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGAcc < 1:1463759/76‑1 (MQ=255)
aTGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTc < 2:1110725/76‑1 (MQ=255)
ggTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCaaa < 2:1531655/76‑1 (MQ=255)
|
GCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAA > REL606/3175252‑3175397
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
