Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A114 F1 I1 R1 20 239.5 15463816 93.1% 14396812 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,163,752 T→G I966S (ATC→AGC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,163,7520TG99.3% 572.9 / NA 135I966S (ATC→AGC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (93/41);  minor base C (0/1);  total (93/42)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

GACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163679‑4163824
                                                                         |                                                                        
gACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGcc                                                                        <  2:7574048/76‑1 (MQ=255)
gACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGcc                                                                        >  1:5053577/1‑76 (MQ=255)
 aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       <  1:790406/76‑1 (MQ=255)
 aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       <  2:6724697/76‑1 (MQ=255)
 aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       >  2:4694160/1‑76 (MQ=255)
   aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     <  2:648931/76‑1 (MQ=255)
      cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  >  2:7224022/1‑76 (MQ=255)
        tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg                                                                <  2:2603875/76‑1 (MQ=255)
        tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg                                                                <  1:316943/76‑1 (MQ=255)
           gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt                                                             >  1:2098427/1‑76 (MQ=255)
           gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt                                                             <  1:2202439/76‑1 (MQ=255)
            cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                            >  1:4923706/1‑76 (MQ=255)
            cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                            >  2:1796647/1‑76 (MQ=255)
            cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                            >  2:3986885/1‑76 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           <  2:5987436/76‑1 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           >  2:5589085/1‑76 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           >  2:1369184/1‑76 (MQ=255)
             tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           <  1:6415919/76‑1 (MQ=255)
              ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                          <  2:7640588/76‑1 (MQ=255)
              ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                          >  1:4467493/1‑76 (MQ=255)
              ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                          >  2:356114/1‑76 (MQ=255)
               gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCttt                                                         <  2:3989386/76‑3 (MQ=255)
               gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGt                                                         >  2:7039827/1‑76 (MQ=255)
                 aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTc                                                       >  2:6667880/1‑76 (MQ=255)
                 aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTc                                                       <  2:6344531/76‑1 (MQ=255)
                  aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      <  1:4132946/76‑1 (MQ=255)
                  aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      >  1:4589913/1‑76 (MQ=255)
                   tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg                                                     <  2:2741157/76‑1 (MQ=255)
                   tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg                                                     >  2:5853613/1‑76 (MQ=255)
                   tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg                                                     <  2:6884104/76‑1 (MQ=255)
                    cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc                                                    >  2:5419575/1‑76 (MQ=255)
                    cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGc                                                    <  1:140096/76‑1 (MQ=255)
                     gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGcc                                                   >  1:6168326/1‑76 (MQ=255)
                       agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGt                                                 <  1:1980640/76‑1 (MQ=255)
                        gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTa                                                <  2:5850690/76‑1 (MQ=255)
                        gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTa                                                >  2:2508227/1‑76 (MQ=255)
                        gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTa                                                >  1:728077/1‑76 (MQ=255)
                         aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               >  2:1239236/1‑76 (MQ=255)
                         aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               >  1:7297903/1‑76 (MQ=255)
                          aaTGCAGCTAAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATc                                              <  2:5548061/76‑1 (MQ=255)
                          aaTGCAGCTAAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGACCCTCGAAGCGGGTCTGTTCAGCCGTATc                                              <  2:1737856/76‑1 (MQ=255)
                           aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATcc                                             <  1:984127/76‑1 (MQ=255)
                            tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCg                                            >  1:13064/1‑76 (MQ=255)
                            tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCg                                            >  1:4765535/1‑76 (MQ=255)
                             gCAGCTAAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgt                                           <  1:5899574/76‑1 (MQ=255)
                               aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc                                         >  1:1416193/1‑76 (MQ=255)
                                gCTAAAACGGGCGAAAAAAGCCCTGTCTGAAAAACTGCAGAGCCTCAAAGCGGGTCTTTTCAGCCGTATCCgtgct                                        <  2:4830456/76‑1 (MQ=255)
                                 cTCAAACGGGCGAAGAAAGCCCTGTCGAAAAAACTGCAGGCCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctg                                       <  2:7313817/76‑1 (MQ=255)
                                  tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  2:3397909/1‑76 (MQ=255)
                                   cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  2:6136270/1‑76 (MQ=255)
                                   cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  2:408915/1‑76 (MQ=255)
                                   aaaaCGGGCGAAGAAAGCCCTGTCTGAAGAACGGCAGACCCTCGAAGGGGGTCTGTCCAGCCGTATCCgtgctgtg                                     <  2:6404118/75‑1 (MQ=255)
                                     aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   >  2:5742357/1‑76 (MQ=255)
                                     aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   <  1:2401476/76‑1 (MQ=255)
                                     aaCAGGCGAAAAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   <  1:959026/76‑1 (MQ=255)
                                       cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg                                 >  1:3245863/1‑76 (MQ=255)
                                        aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                <  2:1820715/76‑1 (MQ=255)
                                        aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                >  2:6110185/1‑76 (MQ=255)
                                         ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               <  1:1791967/76‑1 (MQ=255)
                                         ggCGAAAAAAGACCTGTCAGAAGACCTGCAGGGCCGCAAAGGGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               <  2:5057954/76‑1 (MQ=255)
                                          gCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  1:2692499/76‑1 (MQ=255)
                                           cGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc                             <  1:1302441/76‑1 (MQ=255)
                                            gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  1:2841647/1‑76 (MQ=255)
                                            gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  1:1023504/1‑76 (MQ=255)
                                             aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:7118870/1‑76 (MQ=255)
                                             aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:5299303/1‑76 (MQ=255)
                                               gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         >  1:2002185/1‑76 (MQ=255)
                                                aaagcCCTGTCTAAAGACCTGCGGGCCCTCGAGGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        <  1:2508227/76‑1 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:3604642/1‑76 (MQ=255)
                                                 aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTCTTCAGCCGTATCCGTGCTGTGCTGGCAGCcggcgg                       >  1:4846805/1‑76 (MQ=255)
                                                  agcccTGTCGAAAGAACGGCAGACCCGCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      <  1:6857787/73‑1 (MQ=255)
                                                  agaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      >  1:3911934/1‑76 (MQ=255)
                                                  agaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      >  1:4545425/1‑76 (MQ=255)
                                                   gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  2:5101140/1‑76 (MQ=255)
                                                   gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  1:6687910/1‑76 (MQ=255)
                                                      cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg                  >  1:6543104/1‑76 (MQ=255)
                                                      cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg                  >  1:1700024/1‑76 (MQ=255)
                                                      cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGAAGCCGGGGGCGTTg                  >  1:1459140/1‑76 (MQ=255)
                                                       tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 >  1:6946347/1‑76 (MQ=255)
                                                       tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGCTGa                 >  2:2382498/1‑76 (MQ=255)
                                                        gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGGTGaa                >  2:390536/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  2:5109185/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGGTGAAGc              >  1:5873877/1‑76 (MQ=255)
                                                           tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:3479624/1‑76 (MQ=255)
                                                           tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:6850014/1‑76 (MQ=255)
                                                           tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGCTGAAGCt             >  2:6523223/1‑76 (MQ=255)
                                                           tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCAGGTGGCGTTGCAGCt             >  1:4047637/1‑76 (MQ=255)
                                                            gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:7200464/1‑76 (MQ=255)
                                                            aaagatCCGCAGAGCCTCGAGGGGGTTCTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            <  2:3824902/75‑1 (MQ=255)
                                                              agaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          >  2:1258275/1‑76 (MQ=255)
                                                              agaaCCGCAGCGCCGCAAAGCGGGTCTTTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          <  1:1651976/76‑1 (MQ=255)
                                                               gaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga         >  2:6939272/1‑76 (MQ=255)
                                                                 aCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       >  1:110966/1‑76 (MQ=255)
                                                                  cTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      >  2:2852790/1‑76 (MQ=255)
                                                                     cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  1:5166123/1‑76 (MQ=255)
                                                                     cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  1:3789282/1‑76 (MQ=255)
                                                                     cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  1:2223444/1‑76 (MQ=255)
                                                                      agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  2:2006670/1‑76 (MQ=255)
                                                                      agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:6015983/1‑76 (MQ=255)
                                                                      agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  2:962025/1‑76 (MQ=255)
                                                                         |                                                                        
GACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163679‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A