Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F1 I1 R1
|
14 |
46.3 |
3075166 |
96.7% |
2973685 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,752 |
T→G |
I966S (ATC→AGC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,752 | 0 | T | G | 95.0%
| 152.6
/ ‑4.2
| 40 | I966S (ATC→AGC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (22/16); total (23/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
CAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCG > REL606/4163681‑4163823
|
cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc > 2:60888/1‑76 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg > 1:1470045/1‑76 (MQ=255)
aaaCGTGCGATGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg > 2:1317909/1‑76 (MQ=255)
aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa < 1:522095/76‑1 (MQ=255)
cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg > 1:818749/1‑76 (MQ=255)
gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGc > 1:1359763/1‑76 (MQ=255)
tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg > 1:306853/1‑76 (MQ=255)
cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc < 2:709109/76‑1 (MQ=255)
cTGAAAATCAAAGAAAGCCAACTCAAACAGGCGAAGAAAGACCTTTCTGAAGAACTGCAGATCCTCGAAGCGGGTc < 2:709093/76‑1 (MQ=255)
tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt > 2:741502/1‑76 (MQ=255)
tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt < 2:1099332/76‑1 (MQ=255)
aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTc < 1:240844/76‑1 (MQ=255)
aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa < 1:1060089/76‑1 (MQ=255)
aTCGAAGAAAGTCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa < 2:109817/76‑1 (MQ=255)
tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg < 2:932903/76‑1 (MQ=255)
tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg < 1:1228453/76‑1 (MQ=255)
gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTATGTTCAGcc > 1:51724/1‑76 (MQ=255)
aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt < 1:1249115/76‑1 (MQ=255)
aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATcc < 1:604985/76‑1 (MQ=255)
aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATcc > 2:504135/1‑76 (MQ=255)
cTCAAACAGGCGAAGAAAGACCTCTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGCATCCgagctg > 1:577310/1‑76 (MQ=255)
aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc > 2:97656/1‑76 (MQ=255)
agcAGGAGAAGAAAGACCGATCTGAAGAACCGCAGAGCCGCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct < 2:324008/74‑1 (MQ=255)
aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGtgctgtgctg < 2:914802/76‑1 (MQ=255)
cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGTCGTATCCGTGCTGTGCTgg < 2:330805/76‑1 (MQ=255)
cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg > 2:618496/1‑76 (MQ=255)
aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt < 2:997865/76‑1 (MQ=255)
aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt < 2:1128095/76‑1 (MQ=255)
ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa < 1:1237307/76‑1 (MQ=255)
cGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc < 1:849556/76‑1 (MQ=255)
acaaaagtCCTGTCAGAACACCGCCAGACCCCCGACGCGGCTCTGTCCAGCCGTATCCGTGCTGTACTGGTAGCCg < 2:579274/73‑1 (MQ=255)
aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg > 2:953966/1‑76 (MQ=255)
aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg < 1:1050252/76‑1 (MQ=255)
aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 2:1019980/1‑76 (MQ=255)
aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTCCTGGTAGCCGGTgg > 2:237407/1‑76 (MQ=255)
gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg > 2:812255/1‑76 (MQ=255)
ccTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGtt > 2:159266/1‑76 (MQ=255)
cTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg > 2:446779/1‑76 (MQ=255)
gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa > 2:1106885/1‑76 (MQ=255)
aggaaCAGAAGAGCCTCGAACGGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga < 2:162518/74‑1 (MQ=255)
aagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga > 2:307247/1‑76 (MQ=255)
aagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga > 1:814744/1‑76 (MQ=255)
gaaCTCCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga < 2:517175/76‑1 (MQ=255)
tcAGAGCCTCAAAGCGGGTGTTTCCAGCCGTATCCGTGCTGTACTGGTAGCCGGTGGCGTTGAAGCCGAGAAGCTc < 2:1313202/75‑1 (MQ=255)
cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg > 1:267955/1‑76 (MQ=255)
cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg > 2:264930/1‑76 (MQ=255)
|
CAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCG > REL606/4163681‑4163823
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
