Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A135 F1 I1 R1 14 200.0 12709596 94.6% 12023277 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,163,752 T→G I966S (ATC→AGC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,163,7520TG98.6% 598.8 / ‑1.8 139I966S (ATC→AGC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (109/28);  total (111/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 163 total aligned reads displayed.

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824
                                                                        |                                                                         
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                        <  2:3232556/76‑1 (MQ=255)
 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                       >  2:4169196/1‑76 (MQ=255)
 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                       <  2:5441580/76‑1 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      >  2:2202442/1‑76 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      <  1:1361810/76‑1 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                      >  2:1288222/1‑76 (MQ=255)
   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa                                                                     >  1:2036448/1‑76 (MQ=255)
   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa                                                                     <  2:4790029/76‑1 (MQ=255)
   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa                                                                     <  2:2680466/76‑1 (MQ=255)
   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa                                                                     <  1:1607522/76‑1 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                   <  2:5606451/76‑1 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                   <  2:5044130/76‑1 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGACTCGAAg                                                                   >  2:4490530/1‑76 (MQ=255)
      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCCCGAAGc                                                                  >  1:3998954/1‑76 (MQ=255)
      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGc                                                                  >  2:3464651/1‑76 (MQ=255)
      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGc                                                                  <  1:1181693/76‑1 (MQ=255)
       tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg                                                                 <  2:1662759/76‑1 (MQ=255)
        gcgcTGGGAATCGAAGAAATGCAGCTCAAGCAGGCGGAGAAAGGCCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg                                                                <  2:2582946/76‑1 (MQ=255)
        gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg                                                                <  2:4539985/76‑1 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                               >  2:2488738/1‑76 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                               <  2:4047576/76‑1 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                               <  2:5330202/76‑1 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                               >  1:1781771/1‑76 (MQ=255)
          gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt                                                              >  1:2121010/1‑76 (MQ=255)
           cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc                                                             >  1:3840992/1‑76 (MQ=255)
            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                            >  1:4304598/1‑76 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                           >  2:5402553/1‑76 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg                                                           >  2:3966454/1‑76 (MQ=255)
               aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGtt                                                         >  1:2846627/1‑76 (MQ=255)
               aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGtt                                                         <  2:1215451/76‑1 (MQ=255)
                aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑c                                                       >  2:5122034/1‑76 (MQ=255)
                aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑c                                                       <  2:887016/76‑1 (MQ=255)
                aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑c                                                       <  2:11250/76‑1 (MQ=255)
                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑Ca                                                      >  2:1005822/1‑76 (MQ=255)
                  tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAg                                                     <  1:4822709/76‑1 (MQ=255)
                  tCGAAGAAATGCAGATCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGTGGGCCTGttaac                                                      >  2:6268301/1‑73 (MQ=255)
                   cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGc                                                    >  1:2322072/1‑76 (MQ=255)
                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCg                                                  <  1:5209511/76‑1 (MQ=255)
                      agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGt                                                 >  2:1456619/1‑76 (MQ=255)
                         aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATc                                              <  1:1031662/76‑1 (MQ=255)
                          aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATcc                                             >  1:2504664/1‑76 (MQ=255)
                           tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCg                                            >  2:3273433/1‑76 (MQ=255)
                            gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgt                                           >  1:1040728/1‑76 (MQ=255)
                            gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgt                                           <  1:2977366/76‑1 (MQ=255)
                             cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtg                                          >  2:4597215/1‑76 (MQ=255)
                             cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtg                                          >  1:3563811/1‑76 (MQ=255)
                             cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtg                                          >  2:1147749/1‑76 (MQ=255)
                               gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgct                                        >  1:3634827/1‑76 (MQ=255)
                                cTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctg                                       >  1:1448733/1‑76 (MQ=255)
                                cTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctg                                       >  2:5220339/1‑76 (MQ=255)
                                 tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt                                      >  1:4686290/1‑76 (MQ=255)
                                  cAAACGGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCttgctg                                       <  2:5225871/74‑1 (MQ=255)
                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCttgctg                                       >  1:5225871/1‑74 (MQ=255)
                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg                                     >  1:4028449/1‑76 (MQ=255)
                                    aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtgct                                   >  2:942288/1‑76 (MQ=255)
                                      cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTgg                                 >  1:3419762/1‑76 (MQ=255)
                                       aGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt                                <  1:5578518/76‑1 (MQ=255)
                                       aGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt                                <  1:468886/76‑1 (MQ=255)
                                        ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTa                               >  1:5836982/1‑76 (MQ=255)
                                         gaaaagaaagcCCGGTCTGAAGACCTGCAGACCCTCGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:2342732/73‑1 (MQ=255)
                                           gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  1:5553024/1‑76 (MQ=255)
                                           gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  1:4859698/1‑76 (MQ=255)
                                           gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  2:3550687/1‑76 (MQ=255)
                                           gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGCT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  1:1315890/1‑76 (MQ=255)
                                            aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:916504/1‑76 (MQ=255)
                                             agaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  1:1838724/1‑76 (MQ=255)
                                              gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         >  2:1572253/1‑76 (MQ=255)
                                                acgaCCTGTGAGAAGCCCGGCAGA‑CCCCGGAGGGGGTTTTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       <  2:2023286/74‑1 (MQ=255)
                                                aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  1:4194119/1‑76 (MQ=255)
                                                  gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  2:1114075/1‑76 (MQ=255)
                                                  gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  2:5717173/1‑76 (MQ=255)
                                                    ccTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGtt                   >  2:1215982/1‑76 (MQ=255)
                                                      tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 >  1:4795468/1‑76 (MQ=255)
                                                      gttCTGAAGAACGGCAGACCCTGGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 <  1:2884191/74‑1 (MQ=255)
                                                       gTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  2:4755272/1‑76 (MQ=255)
                                                       gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  2:5484825/1‑76 (MQ=255)
                                                        tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  2:5011246/1‑76 (MQ=255)
                                                        tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  1:526213/1‑76 (MQ=255)
                                                        tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  1:4949965/1‑76 (MQ=255)
                                                        gcagaagaaCTGCAGTGCCCGGAAGGGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               <  1:4085329/73‑1 (MQ=255)
                                                         cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:3907385/1‑76 (MQ=255)
                                                          tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:6267259/1‑76 (MQ=255)
                                                          tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:3377288/1‑76 (MQ=255)
                                                           gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:651603/1‑76 (MQ=255)
                                                           gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  1:6135010/1‑76 (MQ=255)
                                                           gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:1766470/1‑76 (MQ=255)
                                                             agaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          >  2:2904902/1‑76 (MQ=255)
                                                             aaaaCTGCAGAGCCTCGAAGGGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          <  1:3316125/74‑1 (MQ=255)
                                                              gaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga         >  1:2174235/1‑76 (MQ=255)
                                                              aaccTGCGGAGCCTGGAAGGGGTTTTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga         <  1:1134941/73‑1 (MQ=255)
                                                              aaccTGCGGAGCCTCGAGGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga         <  1:4451891/73‑1 (MQ=255)
                                                                aCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       >  1:5915409/1‑76 (MQ=255)
                                                                 cTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      >  1:5906836/1‑76 (MQ=255)
                                                                  tGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGCAGCt     >  2:3769971/1‑76 (MQ=255)
                                                                  tGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGGGGCGTTGAAGCTGAGAAGCt     >  1:497154/1‑76 (MQ=255)
                                                                  ggCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     <  1:6318704/75‑1 (MQ=255)
                                                                   gCAGAGCCTCGAAGGGGGTTTTTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    <  1:3702656/76‑1 (MQ=255)
                                                                   gCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    >  2:1624522/1‑76 (MQ=255)
                                                                   gCAGACCCTCGAAGGGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    <  1:488055/76‑1 (MQ=255)
                                                                     agagCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:2220661/1‑76 (MQ=255)
                                                                        |                                                                         
ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A