Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A64 F1 I1 R1
|
17 |
58.5 |
3743480 |
96.8% |
3623688 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,165,450 |
C→A |
R131L (CGC→CTC) |
yehU ← |
predicted sensory kinase in two‑component system with YehT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,165,450 | 0 | C | A | 100.0%
| 138.9
/ NA
| 38 | R131L (CGC→CTC) | yehU | predicted sensory kinase in two‑component system with YehT |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/25); total (13/25) |
ATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGCGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAG > REL606/2165378‑2165520
|
aTTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGAc > 2:1494322/1‑76 (MQ=255)
tttGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACg > 1:1355546/1‑76 (MQ=255)
tttGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACg < 1:1280607/76‑1 (MQ=255)
ttGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACgg < 2:836977/76‑1 (MQ=255)
tGCACCATTTCAGCGACGAACGTGACTGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGa < 2:174202/76‑1 (MQ=255)
gCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGAt < 2:1201793/76‑1 (MQ=255)
cACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATc < 1:1562837/76‑1 (MQ=255)
aCCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCa > 1:232091/1‑76 (MQ=255)
tttCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGATACGGATCAGGAt < 2:602305/76‑1 (MQ=255)
aGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCtg > 2:1406510/1‑76 (MQ=255)
gacgaACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTgtgt > 1:754377/1‑76 (MQ=255)
gacgaACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTgtgt < 2:1019960/76‑1 (MQ=255)
acgaACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGGTCAGGATGCTGTGTa > 2:943656/1‑76 (MQ=255)
ggACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGgcc < 1:870152/75‑1 (MQ=255)
aCCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGc < 2:1724022/76‑1 (MQ=255)
cggcggTAATGGAGTTAAAGACTTTATCAGTGCGCTCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCaa > 1:449662/1‑76 (MQ=255)
gcggTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATc < 1:4740/76‑1 (MQ=255)
cggTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATcc < 2:1622981/76‑1 (MQ=255)
gTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCtt < 2:751492/76‑1 (MQ=255)
gTAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCtt < 1:286496/76‑1 (MQ=255)
tAATGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTc < 1:688361/76‑1 (MQ=255)
aaTGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCa < 1:469894/76‑1 (MQ=255)
aTGGAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCaa > 2:1130501/1‑76 (MQ=255)
gggAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCTAGCAATCCTTCAAc < 1:64829/75‑1 (MQ=255)
ggAGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACg > 2:958923/1‑76 (MQ=255)
aGTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGAt < 1:872782/76‑1 (MQ=255)
aGTTAAAGACTTTATCAGCGCGCCAGAGACGGATCAGGATGCTGTGTACCAGGCCGCCCAGCAATCATTCAACGAt > 2:717824/1‑76 (MQ=255)
gTTAAAGCCTCTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATg < 2:496049/76‑1 (MQ=255)
gTTAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATg > 2:1668646/1‑76 (MQ=255)
ttAAAGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATgg > 2:1542045/1‑76 (MQ=255)
aaaGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGtt > 2:176911/1‑76 (MQ=255)
aaaGACTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGtt < 2:834777/76‑1 (MQ=255)
cTTTATCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGAt < 2:1691517/76‑1 (MQ=255)
tCAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGc < 1:904781/76‑1 (MQ=255)
cAGTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCa > 1:1499093/1‑76 (MQ=255)
gTGCGCCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAAc > 1:801539/1‑76 (MQ=255)
gcgcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACtt < 1:309301/76‑1 (MQ=255)
cgcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTa < 2:1645635/76‑1 (MQ=255)
cgcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTa < 2:92307/76‑1 (MQ=255)
ggccGTGACGGATCGGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAg < 2:338888/74‑1 (MQ=255)
gcCCGAGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAg < 1:888514/76‑1 (MQ=255)
gcCCGAGACGGATCAGGATGCTGAGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAg > 2:67909/1‑76 (MQ=255)
|
ATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGAGTTAAAGACTTTATCAGTGCGCCCGCGACGGATCAGGATGCTGTGTACCAGGCCGCCGAGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAG > REL606/2165378‑2165520
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
