Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A108 F1 I1 R1
|
80 |
195.1 |
12639756 |
93.3% |
11792892 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,145,830 |
A→G |
intergenic (+161/‑34) |
dnaG → / → rpoD |
DNA primase/RNA polymerase sigma factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,145,830 | 0 | A | G | 99.4%
| 684.8
/ ‑4.1
| 176 | intergenic (+161/‑34) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (77/98); total (77/99) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 |
Only 100 of 193 total aligned reads displayed.
AATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTC > REL606/3145757‑3145902
|
aaTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCCACGcc > 2:2155495/1‑76 (MQ=255)
aaTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGcc > 1:4102227/1‑76 (MQ=255)
aaTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGcc > 1:3330448/1‑76 (MQ=255)
aTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCCACGCCa > 2:2061101/1‑76 (MQ=255)
aTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCa > 1:1432641/1‑76 (MQ=255)
aTTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAAACGCCCAACGccc > 2:4858928/1‑75 (MQ=255)
ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa < 1:4440897/76‑1 (MQ=255)
ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa > 1:5263777/1‑76 (MQ=255)
ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa < 1:6057170/76‑1 (MQ=255)
ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCaa > 1:2440728/1‑76 (MQ=255)
tATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTGAATCGCCCAAAGCCAAc > 1:3128815/1‑76 (MQ=255)
tATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAAc < 1:1273400/76‑1 (MQ=255)
tCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCt > 1:3763594/1‑76 (MQ=255)
tCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCt > 1:5536758/1‑76 (MQ=255)
tCGTTGGCGGTAAACAACCGTGGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCt < 2:4254869/76‑1 (MQ=255)
cGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTc < 1:1095237/76‑1 (MQ=255)
cGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTc > 1:2197722/1‑76 (MQ=255)
gTTGGCGTAAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGCCATCGGGTCAATCGCCCAACGCCAACCTCa < 2:591615/76‑1 (MQ=255)
gTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCa < 2:2749286/76‑1 (MQ=255)
gTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGGCAATCGCCCAACGCCAACCTCa > 2:4193536/1‑76 (MQ=255)
ttGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCCACGCCAACCTCAt > 1:1600447/1‑76 (MQ=255)
ttGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCAt < 1:1092926/76‑1 (MQ=255)
tGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATg < 1:196032/76‑1 (MQ=255)
tGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATg < 1:3482419/76‑1 (MQ=255)
ggCGGTAAACAACCGTTGGATTTCAGCGTTAACTGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGa < 1:757340/76‑1 (MQ=255)
ggCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGa < 1:3700513/76‑1 (MQ=255)
cGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGaaa > 1:122607/1‑76 (MQ=255)
cGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGaaa < 1:4254002/76‑1 (MQ=255)
ggTAAACAACCGTTGGATTTCAGCTTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAAt < 1:530774/76‑1 (MQ=255)
gTAAACAGCCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATa < 2:49072/76‑1 (MQ=255)
gTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATa > 1:5084496/1‑76 (MQ=255)
tAAACAACCGTTGGATTTCAGCGTTAACGGCTGGAGGAGATCGGGGCCATCGCCCCACACCCAACACATGAAATaa > 2:4945919/1‑76 (MQ=255)
tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATaa < 2:1791645/76‑1 (MQ=255)
tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATaa < 2:3178052/76‑1 (MQ=255)
tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATaa < 2:3829271/76‑1 (MQ=255)
aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAg > 2:539580/1‑76 (MQ=255)
aacaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAgt < 1:4662545/76‑1 (MQ=255)
acaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAgtg > 1:5156059/1‑76 (MQ=255)
caacCGTTGGATTTCAGCGTTAACGGCTGGAGGACATCGGGTGAATCGCCCAACGCCAACCTCATGAAATAAgtgt > 2:909837/1‑76 (MQ=255)
caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAgtgt < 2:3128815/76‑1 (MQ=255)
caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGACAATCGCCCAACGCCAACCTCATGAAATAAgggt > 1:3557684/1‑76 (MQ=255)
aacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGtgtg < 1:2941390/76‑1 (MQ=255)
acCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTgg > 2:5344503/1‑76 (MQ=255)
cGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGAt > 1:2085013/1‑76 (MQ=255)
cGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGGCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGaa > 2:4729770/1‑75 (MQ=255)
gTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATa < 2:778496/76‑1 (MQ=255)
tGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAcc > 1:3470072/1‑76 (MQ=255)
tGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAcc < 2:1358119/76‑1 (MQ=255)
gATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGt < 2:3618815/76‑1 (MQ=255)
gATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGt > 1:3638035/1‑76 (MQ=255)
gATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAACGt > 1:4835032/1‑76 (MQ=255)
ttCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACGGTCtt < 2:3557684/76‑1 (MQ=255)
ttCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCtt < 2:3579198/76‑1 (MQ=255)
ttCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCtt < 1:4354304/76‑1 (MQ=255)
tCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTa > 1:3196675/1‑76 (MQ=255)
tCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTa < 1:3865693/76‑1 (MQ=255)
cAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTAt > 1:4743354/1‑76 (MQ=255)
gCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATgg < 1:1565729/76‑1 (MQ=255)
gCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATgg < 2:2592794/76‑1 (MQ=255)
cGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATAACGTCTTAttgg > 1:1633000/1‑73 (MQ=255)
gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAg > 1:3038274/1‑76 (MQ=255)
gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAg < 1:2142811/76‑1 (MQ=255)
ttAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGc < 1:862022/76‑1 (MQ=255)
ttAACGGCTGAAGGACATCGGGCCAATCGCCCAACGCCAACCTCATAAAATAAGTGTGGATACCGTCTTTTGGAGc > 1:267311/1‑76 (MQ=255)
ttAACGGCTGAAAGACACGGGGTCAATCGGCCAACGCCAACCTCATGAAATAAGTGGGGAGACCCGCTTTTGGTGc > 2:3302701/1‑76 (MQ=255)
tAACGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCa < 1:6261543/76‑1 (MQ=255)
aaCGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaa < 1:2541277/76‑1 (MQ=255)
aCGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 2:5475659/76‑1 (MQ=255)
aCGGCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 2:4361029/76‑1 (MQ=255)
ggCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 1:4482323/76‑1 (MQ=255)
ggCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 2:3588364/76‑1 (MQ=255)
ggCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 1:6179355/76‑1 (MQ=255)
gCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAcc > 2:4619093/1‑76 (MQ=255)
gCTGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCCTCTTATGGAGCAAAAcc > 2:1138978/1‑76 (MQ=255)
tGAAGGACATCGGGTCAGTCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCg > 1:132667/1‑76 (MQ=255)
tGAAGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCg > 2:398642/1‑76 (MQ=255)
aaGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCa > 1:1811629/1‑76 (MQ=255)
aaGGACAGTGGGTCAATCGCCCAACGCCAGCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCa < 2:5614460/76‑1 (MQ=255)
aGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAg > 1:2556708/1‑76 (MQ=255)
aGGACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAg < 2:3328014/76‑1 (MQ=255)
ggACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGt > 1:57711/1‑76 (MQ=255)
gACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc > 1:4756699/1‑76 (MQ=255)
gACATCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc < 1:2049022/76‑1 (MQ=255)
aTCGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcaca < 1:9703/76‑1 (MQ=255)
cGGGTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGGCACAGc > 2:1609549/1‑76 (MQ=255)
ggTCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCCCAGTCACAGCTg > 2:2140373/1‑76 (MQ=255)
tCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa < 2:6281350/76‑1 (MQ=255)
tCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa > 1:381630/1‑76 (MQ=255)
tCAATCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa < 1:4193536/76‑1 (MQ=255)
aTCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCCGTCACAGCTGAAAct > 1:2747108/1‑76 (MQ=255)
aTCGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAAct < 1:1504932/76‑1 (MQ=255)
cGCCCAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGGGCAAAAACCGCAGTCACAGCTGAAActtc > 2:2800316/1‑76 (MQ=255)
cccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt > 1:2038278/1‑76 (MQ=255)
cccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt < 2:1966356/76‑1 (MQ=255)
cccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt < 2:2555510/76‑1 (MQ=255)
ccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGGGCAAAACCCGCAGTCACAGCTGAAActtcttt > 2:555271/1‑75 (MQ=255)
ccAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTg < 2:4785989/76‑1 (MQ=255)
cAACGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGt < 2:1169815/76‑1 (MQ=255)
aaCGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTc > 2:5206409/1‑76 (MQ=255)
aaCGCCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTc > 2:2946370/1‑76 (MQ=255)
|
AATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTC > REL606/3145757‑3145902
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
