Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A108 F1 I1 R1
|
80 |
195.1 |
12639756 |
93.3% |
11792892 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,589,641 |
G→A |
E307K (GAA→AAA) |
yhiU → |
multidrug resistance efflux transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,589,641 | 0 | G | A | 98.5%
| 794.5
/ ‑0.2
| 200 | E307K (GAA→AAA) | yhiU | multidrug resistance efflux transporter |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (111/86); total (113/87) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 |
Only 100 of 209 total aligned reads displayed.
CTTGCTGCCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACG > REL606/3589568‑3589714
|
cTTGCTGCCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGaaa < 2:4122247/76‑1 (MQ=255)
tgctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAgg > 1:943311/1‑76 (MQ=255)
tgctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAgg > 1:1823042/1‑76 (MQ=255)
gctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGc > 1:1593307/1‑76 (MQ=255)
ctgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCg > 2:4948217/1‑76 (MQ=255)
tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 2:4528355/1‑76 (MQ=255)
tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 2:1105294/1‑76 (MQ=255)
tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 1:5940331/1‑76 (MQ=255)
tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt < 1:5479965/76‑1 (MQ=255)
tgcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGt > 1:4505925/1‑76 (MQ=255)
gcCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACCGGTGCCGCAGAAAGGCGTc > 2:3722261/1‑76 (MQ=255)
ccTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCa < 1:4134064/76‑1 (MQ=255)
tGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCAcc < 2:1593307/76‑1 (MQ=255)
ggCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCAccc < 2:6000944/76‑1 (MQ=255)
gaaTGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCca < 2:5264148/74‑1 (MQ=255)
cATTTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcac > 2:3434310/1‑76 (MQ=255)
cATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcac > 1:4571284/1‑76 (MQ=255)
aTGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcaca > 2:5239375/1‑76 (MQ=255)
aTGTACGTCACGGCATTAGTGGATGAAGGTAGCCCCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCcaca < 1:3884676/76‑1 (MQ=255)
tGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACaa > 2:2103253/1‑76 (MQ=255)
gTACGTCACGGCTTTAGGGGAGGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAAc < 2:3280322/76‑1 (MQ=255)
gTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAAc > 2:2275195/1‑76 (MQ=255)
tgcGTCACGGCATTAGGGGATAAGGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACg < 2:3519776/74‑1 (MQ=255)
tACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACg > 2:4800325/1‑76 (MQ=255)
aCGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCCCCCACAACGc > 1:1343239/1‑76 (MQ=255)
cGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGcc > 2:1313106/1‑76 (MQ=255)
gTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGccc > 1:5519103/1‑76 (MQ=255)
tCACGGCTTTAGTGGAGGAGGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCa < 2:2505793/76‑1 (MQ=255)
tCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCa > 2:1911979/1‑76 (MQ=255)
tCACGGCATTAGGGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCa < 1:1048290/76‑1 (MQ=255)
cACGGCGTTAGGGGATAAAGGTAACCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAg < 1:4638436/76‑1 (MQ=255)
cACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAg < 1:6200931/76‑1 (MQ=255)
aCGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAgg > 1:3421234/1‑76 (MQ=255)
cGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAggg < 2:4597730/76‑1 (MQ=255)
ggCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAgggg > 2:5078290/1‑75 (MQ=255)
gCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTa > 2:4282752/1‑76 (MQ=255)
gCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGACCAGGGTa > 1:3918330/1‑76 (MQ=255)
gCATTAGTGGAGGAAGTTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTa < 1:1747909/76‑1 (MQ=255)
gCATAAGTGGATGAAGTTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGGCACCCACAACGCCCAGGGTa < 2:2600852/76‑1 (MQ=255)
cATTGGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTaa < 1:3557023/76‑1 (MQ=255)
aTTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTaaa > 2:4023798/1‑76 (MQ=255)
ttAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAg < 2:3107005/76‑1 (MQ=255)
ttAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAg > 1:3146076/1‑76 (MQ=255)
tAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGc > 1:1364396/1‑76 (MQ=255)
tAGTGGATAAGGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGc < 1:4820109/76‑1 (MQ=255)
aGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCa > 2:2837495/1‑76 (MQ=255)
gTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCaa > 1:3119585/1‑76 (MQ=255)
ggATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACg < 2:3050399/76‑1 (MQ=255)
gATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACgg > 1:2234739/1‑76 (MQ=255)
gATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACgg > 1:6073575/1‑76 (MQ=255)
aTGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgc > 2:3369623/1‑76 (MQ=255)
aTGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgc > 1:1645027/1‑76 (MQ=255)
tGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACTCACAACGCCCAGGGTAAAGCAACGgcg > 2:5304533/1‑76 (MQ=255)
tGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgcg > 1:5989949/1‑76 (MQ=255)
tGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGgcg > 2:4891587/1‑76 (MQ=255)
agggTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCt < 2:3340405/74‑1 (MQ=255)
aaGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAACAACGGCGCt > 1:5112553/1‑76 (MQ=255)
aGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTc < 2:5731784/76‑1 (MQ=255)
gTAGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCAt > 2:1534548/1‑76 (MQ=255)
aGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTc > 1:6142640/1‑76 (MQ=255)
aGCCGCCAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTc > 1:5162908/1‑76 (MQ=255)
gccgccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCt < 1:2933074/76‑1 (MQ=255)
cgccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTgg < 1:155212/76‑1 (MQ=255)
ccAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGAt < 1:722588/76‑1 (MQ=255)
ccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGAt < 1:2444411/76‑1 (MQ=255)
ccAGAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGAt < 1:6157845/76‑1 (MQ=255)
gAATGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATaaa > 2:121958/1‑76 (MQ=255)
aaTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAg < 1:5336485/76‑1 (MQ=255)
aTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGa < 1:663002/76‑1 (MQ=255)
aTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGa > 1:1402877/1‑76 (MQ=255)
aTGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGa > 1:278110/1‑76 (MQ=255)
tGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGAc < 2:4842923/76‑1 (MQ=255)
tGTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGAc > 1:6307936/1‑76 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 2:5950156/76‑1 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 1:1197645/76‑1 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg > 2:6282400/1‑76 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 2:4109453/76‑1 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 1:1722153/76‑1 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg > 1:2424138/1‑76 (MQ=255)
gTATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACg < 1:3369623/76‑1 (MQ=255)
tATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGa > 2:195825/1‑76 (MQ=255)
tATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGa > 1:2112915/1‑76 (MQ=255)
tATTACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGa > 1:2975700/1‑76 (MQ=255)
ttACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATg > 2:4331945/1‑76 (MQ=255)
ttACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATg < 2:1324971/76‑1 (MQ=255)
tACTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGt > 2:1838511/1‑76 (MQ=255)
cTGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCg < 2:5422793/76‑1 (MQ=255)
tGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGt < 2:2200766/76‑1 (MQ=255)
tGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGt > 2:6213853/1‑76 (MQ=255)
tGGTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGt > 1:4195217/1‑76 (MQ=255)
ggTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTg < 2:2255398/76‑1 (MQ=255)
ggTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTg < 1:395980/76‑1 (MQ=255)
ggTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTg < 2:370323/76‑1 (MQ=255)
gTGCCGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGc > 1:3203774/1‑76 (MQ=255)
ccGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGc > 2:6206305/1‑76 (MQ=255)
ccGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGc < 1:5806647/76‑1 (MQ=255)
cGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCt > 1:3277432/1‑76 (MQ=255)
cGCAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCt < 1:5256641/76‑1 (MQ=255)
cAGAAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTAc > 2:893811/1‑76 (MQ=255)
aGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTAcg > 1:567676/1‑76 (MQ=255)
|
CTTGCTGCCTGGCATGTACGTCACGGCATTAGTGGATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTCATTCTGGATAAAGACGATGTCGTGCAGCTACG > REL606/3589568‑3589714
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
