Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A108 F1 I1 R1 80 195.1 12639756 93.3% 11792892 75.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,985,084 A→G V437A (GTA→GCA)  ECB_03714 ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,985,0840AG98.8% 695.1 / ‑3.1 170V437A (GTA→GCA) ECB_03714conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (55/113);  total (55/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 203 total aligned reads displayed.

TTATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTACCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCG  >  REL606/3985012‑3985156
                                                                        |                                                                         
ttATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCa                                                                        <  2:3460812/76‑1 (MQ=255)
ttATTCATGTCGATGTGTGAAACTTGGTCCAGCAATTCGATTGCAGCGGTAGTGAACGCGCCATAACCAAGTGCCa                                                                        <  2:5052790/76‑1 (MQ=255)
 tATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAg                                                                       <  2:5941885/76‑1 (MQ=255)
  aTTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAgg                                                                      <  2:4102295/76‑1 (MQ=255)
     cATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAAACAAGTGGCAGGTCg                                                                   >  1:4029097/1‑76 (MQ=255)
        gTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGGAGTGGGAGGGCCCTAAACACGGGCCAGGGCGaaa                                                                >  1:4946696/1‑75 (MQ=255)
         tCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGGGTAGTGGGAGGGCCCCAAACAAGTGCCAGGGCGGA‑Ta                                                              >  1:6147279/1‑76 (MQ=255)
         tCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑Ta                                                              <  1:3032444/76‑1 (MQ=255)
          cGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑Taa                                                             <  1:1175673/76‑1 (MQ=255)
          cGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑Taa                                                             <  1:265658/76‑1 (MQ=255)
          cGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGGGGAAGGGCCCACAACAAATGCCAGGGCGAA‑Taa                                                             >  2:2283232/1‑76 (MQ=255)
           gATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAac                                                            <  2:328238/76‑1 (MQ=255)
           gATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTACCAGGTCGAA‑Taat                                                            <  2:1444006/76‑2 (MQ=255)
            aTGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAaca                                                           <  1:626917/76‑1 (MQ=255)
            aTGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAaca                                                           <  1:5837311/76‑1 (MQ=255)
             tgtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAacac                                                          <  2:747379/76‑1 (MQ=255)
             tgtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGGAGTGGGAGGGCCCTAACCAAGTGCCCGGGCGAA‑AAacac                                                          >  2:4342198/1‑76 (MQ=255)
              gtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacg                                                         <  1:5622529/76‑1 (MQ=255)
              gtgtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGGGGAAGGGCCATAAACAAGGTCCCGGGCGAACTAacaa                                                          >  2:4580423/1‑75 (MQ=255)
                gtgAAAGTTTGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacgac                                                       <  1:4990478/76‑1 (MQ=255)
                gtgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGGAGTGGGAGCGCCCTAACCAAATGCCAGGGCGGA‑TAACaagac                                                       >  1:1854283/1‑76 (MQ=255)
                 tgAAAGTTGGTCCAGTAATTCGCTGGCAGGGGTAGTGGGAGCGCCATAAACCAGTGCCCGGGCGAA‑TAACacgccg                                                      >  2:4551672/1‑76 (MQ=255)
                 tgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacgacg                                                      <  1:1075677/76‑1 (MQ=255)
                 tgAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACacgacg                                                      <  2:1625960/76‑1 (MQ=255)
                  gAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACgg                                                     <  2:4299778/76‑1 (MQ=255)
                     aGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGGGCCCTAAACAAGGGCCAGGGCGGA‑TAAAAAGACGGCAg                                                  >  1:4091392/1‑76 (MQ=255)
                      gTTGGTCCAGTAATTCGCTGGCAGGGGGAGTGGAAGGGCCATAAACAAGTGCCACGTCGAA‑AAACACAACGGcggg                                                 >  2:5222280/1‑73 (MQ=255)
                      gTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCCTAAACAAGAGCCAGGGCGAA‑TAACAAGACGGCAgg                                                 >  1:3740339/1‑75 (MQ=255)
                       ttGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAgtg                                                <  2:5759125/76‑1 (MQ=255)
                        tGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAgtgt                                               <  2:134542/76‑1 (MQ=255)
                        tGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAgtgt                                               <  1:1057267/76‑1 (MQ=255)
                           tCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGtgtgtg                                            <  1:3515683/76‑1 (MQ=255)
                            ccAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGa                                           <  2:141044/76‑1 (MQ=255)
                            ccAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGa                                           <  1:4311379/76‑1 (MQ=255)
                             cAGTAATTCGCTGGCAGGCGTAGTGGAAGGGCCATAAACAAAGGCCCGGGCGGA‑TAACACGACGGCCGggtgtggg                                          >  2:4486189/1‑74 (MQ=255)
                             cAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAg                                          <  1:248960/76‑1 (MQ=255)
                               gTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTc                                        <  1:1417151/76‑1 (MQ=255)
                               gTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTc                                        <  1:1496546/76‑1 (MQ=255)
                                  aTTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGtt                                     <  2:4240231/76‑1 (MQ=255)
                                  aTTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGtt                                     <  2:3989784/76‑1 (MQ=255)
                                   ttCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTg                                    <  1:4567026/76‑1 (MQ=255)
                                    tCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGa                                   <  2:1045920/76‑1 (MQ=255)
                                      gCTGGTAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGt                                 <  2:5539736/76‑1 (MQ=255)
                                      gCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGt                                 <  1:3636851/76‑1 (MQ=255)
                                         ggCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCg                              <  2:338494/76‑1 (MQ=255)
                                         ggCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCg                              <  2:2871592/76‑1 (MQ=255)
                                         ggCAGGAGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCg                              <  2:94244/76‑1 (MQ=255)
                                          gCAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGt                             <  2:2991222/76‑1 (MQ=255)
                                           cAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGtt                            <  1:12265/76‑1 (MQ=255)
                                           cAGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGtt                            <  1:6036356/76‑1 (MQ=255)
                                            aGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTg                           <  1:3598235/76‑1 (MQ=255)
                                            aGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTg                           <  1:5810897/76‑1 (MQ=255)
                                            aGGCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTg                           <  1:5555650/76‑1 (MQ=255)
                                              gCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAg                         <  1:907874/76‑1 (MQ=255)
                                              gCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAg                         >  1:5043950/1‑76 (MQ=255)
                                              gCGTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAg                         <  2:3936936/76‑1 (MQ=255)
                                                gTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCa                       <  1:5244110/76‑1 (MQ=255)
                                                gTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCa                       >  1:2460810/1‑76 (MQ=255)
                                                gTAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCa                       >  2:1711099/1‑76 (MQ=255)
                                                 tAGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAg                      <  2:5926701/76‑1 (MQ=255)
                                                  aGTGGAAGCGCCCTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGAGGGTGAGTCGTTGAGTTCGTTTAGCAGa                     >  2:2067913/1‑76 (MQ=255)
                                                  aGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGa                     >  1:5519469/1‑76 (MQ=255)
                                                  aGTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGa                     <  1:2599568/76‑1 (MQ=255)
                                                   gTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGAc                    <  2:5657473/76‑1 (MQ=255)
                                                   gTGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGAc                    <  2:1153007/76‑1 (MQ=255)
                                                    tGGAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACg                   >  1:2520962/1‑76 (MQ=255)
                                                     ggAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGt                  >  2:5667738/1‑76 (MQ=255)
                                                     ggAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGt                  >  1:4093779/1‑76 (MQ=255)
                                                      gAAGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGtt                 <  2:6155877/76‑1 (MQ=255)
                                                       aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg                >  1:1136403/1‑76 (MQ=255)
                                                       aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg                <  1:1821196/76‑1 (MQ=255)
                                                       aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg                <  1:4864829/76‑1 (MQ=255)
                                                       aaGCGCCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTg                >  1:2922270/1‑76 (MQ=255)
                                                          cgcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGAtt             >  2:2557660/1‑76 (MQ=255)
                                                          cgcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGAtt             <  1:3183257/76‑1 (MQ=255)
                                                          cgcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGAtt             <  1:1755885/76‑1 (MQ=255)
                                                           gcCATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTg            <  2:2692966/76‑1 (MQ=255)
                                                            ccATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgc           <  1:517151/76‑1 (MQ=255)
                                                            ccATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgc           >  2:2788451/1‑76 (MQ=255)
                                                            ccATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgc           >  2:1934711/1‑76 (MQ=255)
                                                             cATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcg          >  1:388504/1‑76 (MQ=255)
                                                             cATAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcg          <  1:2211641/76‑1 (MQ=255)
                                                              aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc         <  2:4064436/76‑1 (MQ=255)
                                                              aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc         <  1:3976104/76‑1 (MQ=255)
                                                              aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc         >  1:2132123/1‑76 (MQ=255)
                                                              aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc         >  2:3853835/1‑76 (MQ=255)
                                                              aTAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTgcgc         >  2:5181595/1‑76 (MQ=255)
                                                               tAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCa        >  1:2334170/1‑76 (MQ=255)
                                                               tAACCAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCa        >  1:5700584/1‑76 (MQ=255)
                                                                  ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag     >  2:5469131/1‑76 (MQ=255)
                                                                  ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag     <  2:5492025/76‑1 (MQ=255)
                                                                  ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag     >  2:2445554/1‑76 (MQ=255)
                                                                  ccAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCagag     <  1:5364231/76‑1 (MQ=255)
                                                                   cAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGt    >  2:4853376/1‑76 (MQ=255)
                                                                   cAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGt    >  2:3233076/1‑76 (MQ=255)
                                                                   cAAGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGt    <  2:6282677/76‑1 (MQ=255)
                                                                    aaGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTc   <  1:3117860/76‑1 (MQ=255)
                                                                    aaGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTc   >  1:1646511/1‑76 (MQ=255)
                                                                     aGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCg  >  1:2087170/1‑76 (MQ=255)
                                                                     aGTGCCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCg  >  1:5956289/1‑76 (MQ=255)
                                                                        |                                                                         
TTATTCATGTCGATGTGTGAAAGTTGGTCCAGTAATTCGCTGGCAGGCGTAGTGGAAGCGCCATAACCAAGTACCAGGTCGAA‑TAACACGACGGCAGTGTGTGAGTCGTTGAGTTCGTTGAGCAGACGTTGATTGCGCAGAGTCG  >  REL606/3985012‑3985156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A