Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
19 |
34.1 |
2156969 |
96.4% |
2079318 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,948,122 |
C→T |
intergenic (‑316/+370) |
aslA ← / ← hemY |
acrylsulfatase‑like enzyme/predicted protoheme IX synthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,948,122 | 0 | C | T | 98.3%
| 213.4
/ NA
| 60 | intergenic (‑316/+370) | aslA/hemY | acrylsulfatase‑like enzyme/predicted protoheme IX synthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (38/19); minor base G (1/0); total (41/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGCAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTG > REL606/3948049‑3948194
|
aaCGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAg < 2:804420/76‑1 (MQ=255)
cGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGga < 1:819333/76‑1 (MQ=255)
catttAATATATATGTTATTAATTGANTGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaa > 2:932489/4‑76 (MQ=255)
cAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaa < 1:1045343/76‑1 (MQ=255)
aaTTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaaa > 1:495758/1‑76 (MQ=255)
aaTTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaaa > 1:281246/1‑76 (MQ=255)
aaTTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaaa > 2:647375/1‑76 (MQ=255)
aaTTAATATATATGTTATTAATTGAATGAATTTTAGTATTCATTATCTATATGTATAGAATCGTGCGTAGgagata > 1:119759/1‑74 (MQ=255)
ttAATATATATGTTATTAATTGAATGAATNTTATTATGCATTGTANATATGTGTAGTAGCGTGCGTAGGAGNAnta < 2:912369/76‑1 (MQ=255)
atatatatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTc > 1:672233/1‑76 (MQ=255)
atatatatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTc < 2:137707/76‑1 (MQ=255)
atatatGTTATTAATTGAATGAATTTTACTATTCATTATATATATGTGTAGAATCGTGCGCGGGAGAAATATTCAc > 2:1051263/1‑76 (MQ=255)
tatatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGAAGGAGAAATATTCACt > 2:441543/1‑76 (MQ=255)
atatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTc > 1:620918/1‑76 (MQ=255)
tatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCa < 2:790731/76‑1 (MQ=255)
atGTTATTAATTGAATGAATTTTATTNTTCATTCTATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACCCAg > 2:933676/1‑76 (MQ=255)
atGTTATTAATTGAATGAATTTTATTNTTCATTCTATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACACAg > 2:933683/1‑76 (MQ=255)
gTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGa < 1:573372/76‑1 (MQ=255)
ttattaGTTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGaa > 1:158053/1‑76 (MQ=255)
ttattaATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGaa > 1:580640/1‑76 (MQ=255)
ttattaAATGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGaa > 1:709898/1‑76 (MQ=255)
taATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGtta > 2:400124/1‑76 (MQ=255)
ttGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGttatta > 1:230457/1‑76 (MQ=255)
gaatgaatTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACt > 1:477930/1‑76 (MQ=255)
atgaatTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCa < 2:580640/76‑1 (MQ=255)
aatTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGa < 1:665571/76‑1 (MQ=255)
atTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGaa > 2:64036/1‑76 (MQ=255)
tattatTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCaa > 1:487621/1‑76 (MQ=255)
tattatTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCaa > 1:487633/1‑76 (MQ=255)
tattatTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCaa > 1:324236/1‑76 (MQ=255)
ttattCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAag < 1:704192/76‑1 (MQ=255)
tattCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATCTTCACNCAGGAANTTATTACTCAGGAAGCAAagn > 1:907539/1‑75 (MQ=255)
tattCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGNAAGTTATTACTCAGGAAGCAAaga < 2:188065/76‑1 (MQ=255)
ttCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAgg > 2:356750/1‑76 (MQ=255)
ttCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAgg > 2:356769/1‑76 (MQ=255)
aTTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGAtt > 2:1074324/1‑76 (MQ=255)
ttATCTATATGTGTAGAATCGTGAGGAGTAGATNTATTCACTCAGGAAGTTATTACTNAGGAAGCAAAGAGGATTa > 2:109846/1‑76 (MQ=255)
ttATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGTAGTTATTACTCAGGAAGCAAAGAGGATTa < 1:1054562/76‑1 (MQ=255)
ttATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTa > 1:646802/1‑76 (MQ=255)
ttATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTa > 1:31054/1‑76 (MQ=255)
tatatataTGTGTAGAATCGTGCGTAGGAGAAATATTCGCTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTAc > 1:791324/1‑76 (MQ=255)
tatatatGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAg > 2:1009676/1‑76 (MQ=255)
tatatGTGTAGAATCGTNCGTAGGAGAAATATTCACTCAGGAAGTTATTACNGAGGCAGCAGCGAGGATTACAGaa > 2:892971/1‑76 (MQ=255)
tatGTGCAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAAtt > 1:165244/1‑76 (MQ=255)
tgtgTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTAt > 2:782858/1‑76 (MQ=255)
gtgtAGAATCGTGCGTAGGAGAAATATTCACTCAGGAGGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTAtc > 1:578817/1‑76 (MQ=255)
tgtAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTAtct < 1:802888/76‑1 (MQ=255)
tgtAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAACCAAAGAGGATTACAGAATTAtct < 1:841955/76‑1 (MQ=255)
tAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCa > 1:648209/1‑76 (MQ=255)
aGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCAt < 1:676033/76‑1 (MQ=255)
aGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCAt > 1:414898/1‑76 (MQ=255)
gAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATa > 1:700342/1‑76 (MQ=255)
gAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATa > 1:700321/1‑76 (MQ=255)
gAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATa < 2:594045/76‑1 (MQ=255)
aaTCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATaa > 1:476980/1‑76 (MQ=255)
cGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACaa > 2:591783/1‑76 (MQ=255)
cGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACaa > 1:676748/1‑76 (MQ=255)
tGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgt < 1:585269/76‑1 (MQ=255)
tGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgt < 1:351109/76‑1 (MQ=255)
tGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgt < 1:884405/76‑1 (MQ=255)
gcgtAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgtg < 2:457874/76‑1 (MQ=255)
|
AACGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGCAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTG > REL606/3948049‑3948194
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
