Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A53 F1 I1 R1
|
17 |
63.8 |
4051746 |
97.3% |
3942348 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,876,073 |
T→C |
D89G (GAT→GGT) |
atpC ← |
F0F1 ATP synthase subunit epsilon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,876,073 | 0 | T | C | 98.5%
| 254.5
/ NA
| 65 | D89G (GAT→GGT) | atpC | F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (29/35); total (30/35) |
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGATCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATGCCGCCAGACA > REL606/3876001‑3876145
|
aCGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCt < 2:1347917/76‑1 (MQ=255)
cGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTg < 2:474252/76‑1 (MQ=255)
cGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTg < 2:1672199/76‑1 (MQ=255)
gTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTgg < 1:1744450/76‑1 (MQ=255)
gTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTgg > 1:1471183/1‑76 (MQ=255)
tCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGc > 1:1491297/1‑76 (MQ=255)
cGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGcc < 2:1576499/76‑1 (MQ=255)
gCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCcg < 2:1206671/76‑1 (MQ=255)
cGTGAGAGCTGCTAATGTGCTCTTCTGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCcgcg < 1:1880236/76‑1 (MQ=255)
gTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGa > 1:1418181/1‑76 (MQ=255)
tGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGaa > 2:1935101/1‑76 (MQ=255)
gagaGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAAt > 1:1942824/1‑76 (MQ=255)
gagaGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAAt > 1:1731264/1‑76 (MQ=255)
agagCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAAtt < 2:1230968/76‑1 (MQ=255)
agagCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAAtt < 2:673054/76‑1 (MQ=255)
agCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGc > 1:1132605/1‑76 (MQ=255)
tgctAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGCATTGCggg > 1:136705/1‑75 (MQ=255)
gctAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGgtg < 2:1373435/76‑1 (MQ=255)
ctAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGgtgt > 2:1254350/1‑76 (MQ=255)
ctAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGgtgt < 2:1017904/76‑1 (MQ=255)
ctAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGgtgt < 2:975318/76‑1 (MQ=255)
ctAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGgggt > 1:87549/1‑76 (MQ=255)
tAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTc > 2:465381/1‑76 (MQ=255)
tAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTc > 2:2016315/1‑76 (MQ=255)
tAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTc > 2:1106527/1‑76 (MQ=255)
aaTGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGNCCGCGCACTGCGGTGTCg > 2:782081/1‑76 (MQ=255)
aTGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCgg < 2:1953344/76‑1 (MQ=255)
gCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAg < 2:757962/76‑1 (MQ=255)
tctTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGaa < 2:393937/76‑1 (MQ=255)
tCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACgg > 1:1818214/1‑76 (MQ=255)
cAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGATCCTGGCCGCGAAATACTGTTTCGGCAAGAAcctc > 1:1629902/1‑73 (MQ=255)
tccTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCa < 2:1286306/75‑1 (MQ=255)
ccTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCAc < 1:1857767/76‑1 (MQ=255)
cTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACg < 2:91872/76‑1 (MQ=255)
ttACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGt < 2:481019/76‑1 (MQ=255)
tACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGtt < 1:473347/76‑1 (MQ=255)
tACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGtt < 1:1411628/76‑1 (MQ=255)
gTTTCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGttntc < 2:1418181/76‑4 (MQ=255)
tttCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCa < 1:90021/76‑1 (MQ=255)
tttCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCa > 2:1683057/1‑76 (MQ=255)
tCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAgg > 2:1390345/1‑76 (MQ=255)
tCGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAgg > 2:143014/1‑76 (MQ=255)
cGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGc > 2:91037/1‑76 (MQ=255)
cGCTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGGCACGTTGCCAGGc > 1:286902/1‑76 (MQ=255)
gCTTCCATGGCTCGCGCTTCGTCGAGACCCGGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCt < 2:806012/76‑1 (MQ=255)
cTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGGCACGTTGCCAGGCTg > 2:147224/1‑76 (MQ=255)
cTTCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGCCACGTTGCCAGGCTg > 1:1775210/1‑76 (MQ=255)
ttCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGc < 1:1446581/76‑1 (MQ=255)
tCCGTGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCa < 1:1345447/76‑1 (MQ=255)
tCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCa < 2:598772/76‑1 (MQ=255)
tCCATGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCa < 1:417567/76‑1 (MQ=255)
aTGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACtt < 2:142838/76‑1 (MQ=255)
tGGCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTc < 1:1088174/76‑1 (MQ=255)
gCTCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCaa < 1:1225763/76‑1 (MQ=255)
tCGCGCTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGa < 1:1642279/76‑1 (MQ=255)
gcgcTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAAt < 2:1774352/76‑1 (MQ=255)
cTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATgcc > 2:232101/1‑76 (MQ=255)
cTTCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATgcc > 1:51808/1‑76 (MQ=255)
ttCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATgccg > 2:646595/1‑76 (MQ=255)
ttCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATgccg > 2:1064789/1‑76 (MQ=255)
ttCGTCGAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCGGCACTTCAAGAATgccg > 1:1235128/1‑76 (MQ=255)
cgtcgAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATgccgcc < 1:1866195/76‑1 (MQ=255)
tcgAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATGCCGCcag > 2:745691/1‑76 (MQ=255)
cgAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATGCCGCcaga > 2:666675/1‑76 (MQ=255)
cgAGACCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATGCCGCcaga > 2:1241701/1‑76 (MQ=255)
agaCCCTGGCCGCGAATTGTGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATGCCGCcagaca < 2:1191586/76‑1 (MQ=255)
|
ACGTCGCCGTGAGAGCTGCTAATGTGCTCTTCAGCCTTACGTTTCGCTTCCATGGCTCGCGCTTCGTCGAGATCCTGGCCGCGAATTGCGGTGTCGGCCAGAACGGTCACGTTGCCAGGCTGCACTTCAAGAATGCCGCCAGACA > REL606/3876001‑3876145
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
