Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A53 F1 I1 R1 17 63.8 4051746 97.3% 3942348 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC REL606 4,108,659 (TATTT)1→2 intergenic (‑157/‑120) metJ ← / → metB transcriptional repressor protein MetJ/cystathionine gamma‑synthase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 41086540 (0.000)81 (1.360)
+TATTT		 58/130 0.0 100% intergenic (‑152/‑125) metJ/metB transcriptional repressor protein MetJ/cystathionine gamma‑synthase
?REL606 4108655 = 0 (0.000)intergenic (‑153/‑124) metJ/metB transcriptional repressor protein MetJ/cystathionine gamma‑synthase

AGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4108580‑4108654
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTT  >  REL606/4108655‑4108729
                                                                           |||||                                                                           
AGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCT                                                                                 <  2:1471531/76‑1
   TTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATT                                                                              >  2:1782264/1‑76
   TTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATT                                                                              <  1:1136492/76‑1
   TTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATT                                                                              >  2:1176921/1‑76
   TTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATT                                                                              <  1:133928/76‑1
    TACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTT                                                                             <  1:303183/76‑1
     ACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTT                                                                            <  1:47944/76‑1
     ACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTT                                                                            >  2:1259683/1‑76
     ACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTT                                                                            <  2:1497010/76‑1
     ACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTT                                                                            <  2:1898029/76‑1
        GTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATTTAATTTTAACGGCTATTTTATT                                                                         >  1:1061154/1‑76
         TACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTT                                                                        <  1:216905/76‑1
          ACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTG                                                                       >  2:33864/1‑76
           CAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGG                                                                      >  1:525495/1‑76
           CAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGG                                                                      <  1:140879/76‑1
            AGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGG                                                                     <  2:1386013/76‑1
              GCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGAT                                                                   >  2:1708736/1‑76
                 TTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTG                                                                >  1:717867/1‑76
                  TACAGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGC                                                               >  2:319502/1‑76
                     CGTGACATCGTGTAATGCTCCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCA                                                            <  2:1069643/76‑1
                     CGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCA                                                            <  2:1069641/76‑1
                      GTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGGTTTGGTCAA                                                           >  1:1290995/1‑76
                        GACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATC                                                         >  2:690016/1‑76
                         ACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCT                                                        <  2:1967017/76‑1
                          CATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTA                                                       <  2:201232/76‑1
                           ATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTAT                                                      >  2:1743700/1‑76
                             CGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATAC                                                    <  1:937572/76‑1
                              GTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACG                                                   >  1:1492556/1‑76
                              GTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACG                                                   <  2:729089/76‑1
                               tgcAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGC                                                  >  1:1629219/4‑76
                                GTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGGTTTGCTCAATCTATACGCA                                                 >  1:1696332/1‑76
                                GTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCA                                                 >  2:1361107/1‑76
                                GTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCA                                                 >  2:1108210/1‑76
                                GTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCA                                                 >  1:370662/1‑76
                                 TAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAA                                                >  1:1808286/1‑76
                                   ATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAG                                              <  1:963904/76‑1
                                    TGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGA                                             <  2:1240966/76‑1
                                    TGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGA                                             <  1:1524374/76‑1
                                    TGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGA                                             <  1:817438/76‑1
                                     GCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAA                                            <  2:401349/76‑1
                                      CACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAG                                           <  1:2397/76‑1
                                       ACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGT                                          <  2:966030/76‑1
                                       ACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGT                                          >  2:125637/1‑76
                                         CTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTT                                        <  1:297129/76‑1
                                          TGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTA                                       <  2:1203114/76‑1
                                          TGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTA                                       >  1:215980/1‑76
                                          TGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTA                                       <  1:1036713/76‑1
                                           GTCGGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAG                                      >  2:1533439/1‑76
                                              GGCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATG                                   <  1:472398/76‑1
                                               GCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGT                                  >  2:156474/1‑76
                                               GCGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGT                                  <  2:1144145/76‑1
                                               GCGTGAGAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGT                                  <  2:223204/76‑1
                                                CGTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTC                                 >  2:24382/1‑76
                                                 GTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCC                                <  1:523646/76‑1
                                                 GTGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCC                                >  1:1731334/1‑76
                                                  TGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCA                               >  1:1856468/1‑76
                                                  TGATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCA                               <  1:1436880/76‑1
                                                   GATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAG                              >  1:95062/1‑76
                                                   GATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAG                              >  1:1365202/1‑76
                                                    ATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGA                             >  1:1823055/1‑76
                                                    ATAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGA                             >  2:1397240/1‑76
                                                     TAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGAT                            >  1:259044/1‑76
                                                     TAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGAT                            <  2:171743/76‑1
                                                     TAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGAT                            >  1:804413/1‑76
                                                     TAATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGAT                            <  1:604556/76‑1
                                                      AATGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATG                           <  2:1432886/76‑1
                                                        TGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTA                         >  2:655644/1‑76
                                                        TGCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTA                         >  1:1762847/1‑76
                                                         GCATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTAT                        >  1:1010408/1‑76
                                                           ATATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTG                      <  2:1517303/76‑1
                                                            TATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTaa                     <  2:409975/76‑3
                                                             ATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGAC                    >  1:1128003/1‑76
                                                             ATAATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGAC                    >  1:1127991/1‑76
                                                                ATTTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTC                 >  1:1733300/1‑76
                                                                  TTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCA               <  2:880316/76‑1
                                                                  TTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCA               >  1:239310/1‑76
                                                                  TTTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCA               >  1:1112748/1‑76
                                                                   TTAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCAT              <  2:215295/76‑1
                                                                    TAACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATT             <  2:1443238/76‑1
                                                                     AACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTA            <  1:1536768/76‑1
                                                                     AACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTA            <  1:1924128/76‑1
                                                                      ACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAA           <  2:1516308/76‑1
                                                                      ACGGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAA           <  2:11653/76‑1
                                                                        GGCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACA         <  1:887483/76‑1
                                                                         GCTATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACAC        <  1:1935143/76‑1
                                                                           TATTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAA      <  1:1176921/76‑1
                                                                             TTTTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG    <  1:1493242/76‑1
                                                                               TTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTT  >  1:1524980/1‑76
                                                                               TTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTT  >  1:1690648/1‑76
                                                                           |||||                                                                           
AGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4108580‑4108654
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTT  >  REL606/4108655‑4108729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A