Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A82 F1 I1 R1
|
16 |
52.8 |
3323890 |
97.1% |
3227497 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,404,055 |
T→A |
I21N (ATC→AAC) |
rpsR → |
30S ribosomal protein S18 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,404,055 | 0 | T | A | 98.8%
| 326.8
/ ‑4.4
| 80 | I21N (ATC→AAC) | rpsR | 30S ribosomal protein S18 |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (50/29); total (50/30) |
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCAC > REL606/4403984‑4404127
|
gaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGAc > 1:966137/1‑76 (MQ=255)
aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACt < 1:1088396/76‑1 (MQ=255)
aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACt < 2:744878/76‑1 (MQ=255)
aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACt < 2:1512571/76‑1 (MQ=255)
cTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACta > 1:1493858/1‑76 (MQ=255)
cTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACta > 2:587159/1‑76 (MQ=255)
tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtat > 2:1306556/1‑76 (MQ=255)
tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtat > 1:768561/1‑76 (MQ=255)
aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtata > 2:1175140/1‑76 (MQ=255)
aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtata > 2:1216517/1‑76 (MQ=255)
aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtata > 1:234149/1‑76 (MQ=255)
ccATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCTGAAGGCGTTCAAGAGAACGACTATaaa > 1:1184536/1‑76 (MQ=255)
ccATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATaaa < 2:829196/76‑1 (MQ=255)
atatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGa > 2:1504643/1‑76 (MQ=255)
atatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGa > 1:367969/1‑76 (MQ=255)
atatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGa < 1:210208/76‑1 (MQ=255)
atatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGa > 2:58310/1‑76 (MQ=255)
tatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGat > 1:1580663/1‑76 (MQ=255)
tatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGat > 1:53686/1‑76 (MQ=255)
tatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGat > 2:56001/1‑76 (MQ=255)
atGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGata > 1:1491188/1‑76 (MQ=255)
tGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGatat > 1:847059/1‑76 (MQ=255)
gCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCg < 1:79119/76‑1 (MQ=255)
cACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGc > 2:766609/1‑76 (MQ=255)
aCGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCt < 2:1333674/76‑1 (MQ=255)
cGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTa < 2:1214086/76‑1 (MQ=255)
gTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTAc > 1:727826/1‑76 (MQ=255)
tATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGc > 2:719430/1‑76 (MQ=255)
tATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGc > 1:817449/1‑76 (MQ=255)
aTTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCt > 2:1190972/1‑76 (MQ=255)
aTTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCt < 1:1561142/76‑1 (MQ=255)
aTTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCt < 1:31557/76‑1 (MQ=255)
tttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTg > 1:1379418/1‑76 (MQ=255)
ttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGa > 1:1328626/1‑76 (MQ=255)
ttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGa > 1:303645/1‑76 (MQ=255)
ttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGa > 2:1123328/1‑76 (MQ=255)
ttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGa > 2:1653459/1‑76 (MQ=255)
ttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGa > 1:526684/1‑76 (MQ=255)
tCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaa > 2:811646/1‑76 (MQ=255)
ccGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaaa > 1:1176761/1‑76 (MQ=255)
ccGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaaa < 2:84813/76‑1 (MQ=255)
ccGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaaa > 1:238715/1‑76 (MQ=255)
ccGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaaa > 2:314950/1‑76 (MQ=255)
tcgtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAAc < 1:684388/76‑1 (MQ=255)
tcgtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAAc < 1:560882/76‑1 (MQ=255)
tcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTAc > 2:831234/1‑76 (MQ=255)
tcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTAc < 2:1578465/76‑1 (MQ=255)
gCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACAt < 1:1412906/76‑1 (MQ=255)
gTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCAcc > 2:898697/1‑76 (MQ=255)
ttCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCg < 2:262445/76‑1 (MQ=255)
tCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGa > 1:675076/1‑76 (MQ=255)
tGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaaa < 2:1495185/76‑1 (MQ=255)
gCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAg > 1:457728/1‑76 (MQ=255)
gCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAg < 1:196496/76‑1 (MQ=255)
cGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg < 1:578033/76‑1 (MQ=255)
cGTTACACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg < 1:171133/76‑1 (MQ=255)
ttCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTa < 1:1088483/76‑1 (MQ=255)
tCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTaa > 1:539691/1‑76 (MQ=255)
cACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAACGCGGTAAg > 2:44891/1‑76 (MQ=255)
aCCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGa > 2:923544/1‑76 (MQ=255)
aCCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGa > 1:469823/1‑76 (MQ=255)
cgcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGAtt < 2:1132838/76‑1 (MQ=255)
gcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTg < 2:359929/76‑1 (MQ=255)
gcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTg < 1:451783/76‑1 (MQ=255)
gcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTg < 1:1657607/76‑1 (MQ=255)
gAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTcc < 2:938448/76‑1 (MQ=255)
aaGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTccc < 1:456901/76‑1 (MQ=255)
ggCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCaa < 1:1518040/76‑1 (MQ=255)
gTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGcc > 1:14273/1‑76 (MQ=255)
ttCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg < 1:947187/76‑1 (MQ=255)
ttCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg > 1:210258/1‑76 (MQ=255)
ttCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg < 1:1216517/76‑1 (MQ=255)
ttCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg > 2:1133076/1‑76 (MQ=255)
ttCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg > 1:737345/1‑76 (MQ=255)
tCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGt > 1:1574415/1‑76 (MQ=255)
tCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGt > 2:989930/1‑76 (MQ=255)
cAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTa > 1:1310715/1‑76 (MQ=255)
agagAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATc < 1:134729/76‑1 (MQ=255)
gagaTCGACTATAAAGATATCCCTACGCTGAAAAACTACATCCCCAAAGGCGGTAAGATTTTCCCAAGCCGTATCa < 2:543110/76‑1 (MQ=255)
gagaACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCa > 2:415518/1‑76 (MQ=255)
gagaACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCa < 1:1174484/76‑1 (MQ=255)
agaACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCAc > 2:827471/1‑76 (MQ=255)
agaACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCAc > 1:980839/1‑76 (MQ=255)
|
GACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCAC > REL606/4403984‑4404127
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
