Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
17 |
37.0 |
2377229 |
96.2% |
2286894 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,404,055 |
T→A |
I21N (ATC→AAC) |
rpsR → |
30S ribosomal protein S18 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,404,055 | 0 | T | A | 97.5%
| 132.1
/ NA
| 41 | I21N (ATC→AAC) | rpsR | 30S ribosomal protein S18 |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (16/23); minor base C (0/1); total (17/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCA > REL606/4403982‑4404126
|
gagaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACg < 2:643169/76‑1 (MQ=255)
agaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGa < 1:668362/76‑1 (MQ=255)
gaCTAGCCATATGGTACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGAc < 2:811255/76‑1 (MQ=255)
gaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGAc < 2:14414/76‑1 (MQ=255)
tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtat > 2:380295/1‑76 (MQ=255)
aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtata < 1:1127130/76‑1 (MQ=255)
ccATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATaaa < 2:354060/76‑1 (MQ=255)
cATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAg < 1:52281/76‑1 (MQ=255)
cATATGGCACGNTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAg > 1:166154/1‑76 (MQ=255)
atatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGa > 1:455784/1‑76 (MQ=255)
tatGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGat > 2:585664/1‑76 (MQ=255)
atGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGata > 1:748482/1‑76 (MQ=255)
tGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGatat < 1:32691/76‑1 (MQ=255)
gCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCg < 1:459543/76‑1 (MQ=255)
aCGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCt < 1:244673/76‑1 (MQ=255)
cGTTATTTCCGTCGTCGCAAGTTCTGGCGTTTCNCCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTa < 1:962529/76‑1 (MQ=255)
gTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGACATCGCTAc > 2:1119669/1‑76 (MQ=255)
tCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaa < 1:755212/76‑1 (MQ=255)
cgtcgtCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaaaa < 2:114795/76‑1 (MQ=255)
cgtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACt > 1:697120/1‑76 (MQ=255)
cgtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACt > 1:453128/1‑76 (MQ=255)
cgtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACt > 1:1168420/1‑76 (MQ=255)
gtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTa > 2:808606/1‑76 (MQ=255)
tcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTNCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTAc < 1:968919/76‑1 (MQ=255)
gCAATTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACAt < 2:576814/76‑1 (MQ=255)
cAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAANATATCGCTACGCTGAAAAACTACTTc < 2:1032332/76‑1 (MQ=255)
cAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATc > 2:79844/1‑76 (MQ=255)
ttCTGCCGTTTCATCGCGGAACGCGTTCACGAGACCGACTACAAAGATATCGCTACGCTGAAAAACTGTATCACCg < 2:1068897/76‑1 (MQ=255)
cTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa < 1:835101/76‑1 (MQ=255)
tGCCGTTTCACCGCGGAAGGCGCTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaaa < 1:861183/76‑1 (MQ=255)
gCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAg > 2:424268/1‑76 (MQ=255)
cGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg < 2:453128/76‑1 (MQ=255)
ttCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGCTATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTa < 1:793186/76‑1 (MQ=255)
cgcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTNAGAtt > 2:959009/1‑76 (MQ=255)
cgcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGAtt < 1:380295/76‑1 (MQ=255)
aaGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTccc > 2:907988/1‑76 (MQ=255)
cGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGc < 1:566666/76‑1 (MQ=255)
cGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGc > 1:401297/1‑76 (MQ=255)
gTTCAAGAGAACGACTATAAAGATATCGCTATGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGcc > 2:362780/1‑76 (MQ=255)
aaGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTAt > 2:1144336/1‑76 (MQ=255)
gagaACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCa < 2:615726/76‑1 (MQ=255)
|
GAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATCA > REL606/4403982‑4404126
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
