Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A91 F1 I1 R1
|
18 |
55.4 |
3661525 |
95.1% |
3482110 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,411,844 |
G→T |
F130L (TTC→TTA) |
ECB_04076 ← |
putative oxoacyl reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,411,844 | 0 | G | T | 93.5%
| 150.6
/ 1.8
| 47 | F130L (TTC→TTA) | ECB_04076 | putative oxoacyl reductase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (19/24); total (20/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 |
CTGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTTCGAG > REL606/4411776‑4411915
|
cTGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCCGTAAGCGCTTACTTAATGCCt > 1:1532438/1‑76 (MQ=255)
tGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTg > 2:288375/1‑76 (MQ=255)
tGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCCGCCGTGCCAGCAAACGCCTACTTAATGcccg > 1:822321/1‑74 (MQ=255)
tGACGAAAATAATCGAGCGGTGCGGCAGCGCCGCTTCCGGGTTTGGCTGGGGCAGATCGCGTTTACTTCATGccgg > 2:229277/1‑74 (MQ=255)
aCGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAg < 2:409858/76‑1 (MQ=255)
aaaaTAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGGGCCAGTAAGCGCTTACTTAATGCCTGAGCaa > 2:1383304/1‑76 (MQ=255)
aaaTAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAg < 1:716437/76‑1 (MQ=255)
aaaTAAGCGAGCGGTGCGGCAGGTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAg < 1:700858/76‑1 (MQ=255)
ataatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGga > 2:782509/1‑76 (MQ=255)
ataatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGga < 2:147022/76‑1 (MQ=255)
taatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaa < 2:1607825/76‑1 (MQ=255)
aatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag < 1:1745043/76‑1 (MQ=255)
aatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag < 2:963071/76‑1 (MQ=255)
aatCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag < 1:744417/76‑1 (MQ=255)
aatCGAGAGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaag > 1:1170987/1‑76 (MQ=255)
atCGAGCGGTGCGGCAGCTCCGCTTCCGGGTTCGGCTGTGCCAGTAAGCGATTACTTAATGCCTGAGCAAGgaaga > 2:155790/1‑76 (MQ=255)
tCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaagaa < 1:1755968/76‑1 (MQ=255)
tCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGgaagaa < 1:1184245/76‑1 (MQ=255)
cGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAAc > 2:1092174/1‑76 (MQ=255)
cGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAAc < 1:273386/76‑1 (MQ=255)
gAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGGCAGGAAGCGCCTACTTAATGCCTGAGGAAGggagaaag > 2:386010/1‑74 (MQ=255)
gCGGTGCGGCAGCTCCGCTTCCGGTGTCGGCTGTGCTAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgc < 2:99633/76‑1 (MQ=255)
cGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAATAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgcg > 2:52825/1‑76 (MQ=255)
ggTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTTCTTAATGCCTGAGCCAGGAAGAACGgcgc > 2:722411/1‑76 (MQ=255)
ggTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgcgc < 1:506873/76‑1 (MQ=255)
gTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGgcgcg > 1:197515/1‑76 (MQ=255)
cGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGATGAACGGCGCGCgg < 1:1201612/76‑1 (MQ=255)
ggCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCggg > 1:749012/1‑76 (MQ=255)
gCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGgt < 2:1388768/76‑1 (MQ=255)
gCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGgt < 2:669982/76‑1 (MQ=255)
aGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGgtgt > 1:126987/1‑76 (MQ=255)
cTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTa < 2:1051733/76‑1 (MQ=255)
cGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATg < 2:1744272/76‑1 (MQ=255)
cGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATg > 2:577409/1‑76 (MQ=255)
cTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGc < 2:755966/76‑1 (MQ=255)
tCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCaa < 1:585402/76‑1 (MQ=255)
tttCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGttt > 1:480124/1‑76 (MQ=255)
tttCGGCTGTGCCACTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGttt > 1:983756/1‑76 (MQ=255)
ttCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGtttt > 1:425421/1‑76 (MQ=255)
tCGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTg < 2:1626076/76‑1 (MQ=255)
cGGCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGa < 1:940055/76‑1 (MQ=255)
gCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATc < 1:408576/76‑1 (MQ=255)
gCTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATc < 1:1424458/76‑1 (MQ=255)
cTGTGCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCg > 1:467288/1‑76 (MQ=255)
tgtgCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATTTTTTGATCGa < 2:180660/76‑1 (MQ=255)
gtgCCAGTAAGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGaa > 1:789600/1‑76 (MQ=255)
aaGCGCTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTg > 2:219946/1‑76 (MQ=255)
cgcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGtt < 2:1065530/76‑1 (MQ=255)
cgcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGtt > 1:87350/1‑76 (MQ=255)
cgcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGtt > 1:1083217/1‑76 (MQ=255)
gcTTACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtc > 2:2146/1‑76 (MQ=255)
cttacttaATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtcg < 1:309573/76‑1 (MQ=255)
ttACTCAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtcga < 2:1812099/76‑1 (MQ=255)
tACTTAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTtcgag > 1:905370/1‑76 (MQ=255)
|
CTGACGAAAATAATCGAGCGGTGCGGCAGCTCCGCTTCCGGTTTCGGCTGTGCCAGTAAGCGCTTACTGAATGCCTGAGCAAGGAAGAACGGCGCGCGGGTGTTAATGGCAATGTTTTGATCGAAGCTGTCTGGTTCGAG > REL606/4411776‑4411915
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
